Increased cell killing and DNA damage in cells exposed to ultra-short pulsed electric fields

Stacey, Michael W.; Stickley, J.; Fox, P.; O’Donnell, Christopher J; Schoenbach, K.H.; Beebe, Stephen J.; Buescher, Stephen

doi:10.1109/ceidp.2002.1048741

Cited by 8 publications

(7 citation statements)

References 3 publications

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“…Stacey et al. [ 71 ] registered nsPEF-derived DNA damage by performing a comet assay to evaluate cell survival. To determine if DNA damage was a direct effect of nsPEF, Jurkat cells were lysed and their DNA extracted right after the application of a nsPEF protocol.…”

Section: Nspef-induced Damage In Cell’s Dnamentioning

confidence: 99%

Nanosecond pulsed electric field (nsPEF) and vaccines: a novel technique for the inactivation of SARS-CoV-2 and other viruses?

2022

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Since the beginning of 2020, worldwide attention has been being focussed on SARS-CoV-2, the second strain of the severe acute respiratory syndrome virus. Although advances in vaccine technology have been made, particularly considering the advent of mRNA vaccines, up to date, no single antigen design can ensure optimal immune response. Therefore, new technologies must be tested as to their ability to further improve vaccines. Nanosecond Pulsed Electric Field (nsPEF) is one such method showing great promise in different biomedical and industrial fields, including the fight against COVID-19. Of note, available research shows that nsPEF directly damages the cell’s DNA, so it is critical to determine if this technology could be able to fragment either viral DNA or RNA so as to be used as a novel technology to produce inactivated pathogenic agents that may, in turn, be used for the production of vaccines. Considering the available evidence, we propose that nsPEF may be used to produce inactivated SARS-CoV-2 viruses that may in turn be used to produce novel vaccines, as another tool to address 20 the current COVID-19 pandemic. Key Messages Viral inactivation by using pulsed electric fields in the nanosecond frequency. DNA fragmentation by a Nanosecond Pulsed Electric Field (nsPEF). Opportunity to apply new technologies in vaccine development.

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Section: Nspef-induced Damage In Cell’s Dnamentioning

confidence: 99%

Nanosecond pulsed electric field (nsPEF) and vaccines: a novel technique for the inactivation of SARS-CoV-2 and other viruses?

2022

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“…There are several examples of cell dependence and nsPEF. Stacey et al in 2002 demonstrated that exposing cancer cells to nsPEF with 60 kV/cm could induce DNA damage [ 243 ] ( Figure 5 ). Beebe et al in 2002 studied the antitumor effects of nsPEF on Jurkat cells, with pulses at 60, 150, and 300 kV/cm [ 139 ].…”

Section: Nspef Applicationsmentioning

confidence: 99%

Nanosecond Pulsed Electric Field (nsPEF): Opening the Biotechnological Pandora’s Box

Ruiz-Fernández

Campos

Gutierrez-Maldonado

et al. 2022

IJMS

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Nanosecond Pulsed Electric Field (nsPEF) is an electrostimulation technique first developed in 1995; nsPEF requires the delivery of a series of pulses of high electric fields in the order of nanoseconds into biological tissues or cells. They primary effects in cells is the formation of membrane nanopores and the activation of ionic channels, leading to an incremental increase in cytoplasmic Ca2+ concentration, which triggers a signaling cascade producing a variety of effects: from apoptosis up to cell differentiation and proliferation. Further, nsPEF may affect organelles, making nsPEF a unique tool to manipulate and study cells. This technique is exploited in a broad spectrum of applications, such as: sterilization in the food industry, seed germination, anti-parasitic effects, wound healing, increased immune response, activation of neurons and myocites, cell proliferation, cellular phenotype manipulation, modulation of gene expression, and as a novel cancer treatment. This review thoroughly explores both nsPEF’s history and applications, with emphasis on the cellular effects from a biophysics perspective, highlighting the role of ionic channels as a mechanistic driver of the increase in cytoplasmic Ca2+ concentration.

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“…Other non-thermal effects on the biological material may also appear and affect different biological aspects, such as DNA [40], enzyme activity [41], and cellular proliferation [42]. Actually, most of these investigations have been realized on large amounts of cells.…”

Section: A Non-invasivity Contact Less and Label Freementioning

confidence: 99%

Recent Advances in Microwave-Based Dielectric Spectroscopy at the Cellular Level for Cancer Investigations

Grenier

Dubuc

Chen

et al. 2013

IEEE Trans. Microwave Theory Techn.

167

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Cancer remains a leading cause of death in the world. To overcome this problem, it is necessary to develop new analysis tools, in complementarity to existing ones, to enable the early diagnostic of the diseases, personalized treatment, and further fundamental cancer mechanisms understanding. In this context, micro and millimeterwave-based dielectric spectroscopy performed at the cellular and molecular levels is progressively emerging, as it permits the non-invasive and real time probing of cells in their culture biological medium. The recent advances of this topic are given in this paper with a specific highlight of its various assets.

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Increased cell killing and DNA damage in cells exposed to ultra-short pulsed electric fields

Cited by 8 publications

References 3 publications

Nanosecond pulsed electric field (nsPEF) and vaccines: a novel technique for the inactivation of SARS-CoV-2 and other viruses?

Nanosecond pulsed electric field (nsPEF) and vaccines: a novel technique for the inactivation of SARS-CoV-2 and other viruses?

Nanosecond Pulsed Electric Field (nsPEF): Opening the Biotechnological Pandora’s Box

Recent Advances in Microwave-Based Dielectric Spectroscopy at the Cellular Level for Cancer Investigations

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