Increased 4‐hydroxy‐2‐nonenal‐induced proteasome dysfunction is correlated with cardiac damage in streptozotocin‐injected rats with isoproterenol infusion

Deshpande, Mandar; Mali, Vishal; Pan, Guodong; Yang, Xiao Ping; Thandavarayan, Rajarajan Amirthalingam; Palaniyandi, Suresh S.

doi:10.1002/cbf.3195

Cited by 9 publications

(7 citation statements)

References 39 publications

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“…In addition, the present results identified that the mRNA and protein expression of NOX2 and NOX4 were significantly increased following AB surgery. As a lipid peroxide formed by the lipid peroxidation reaction between ROS and biomacromolecules, 4-HNE exerts a destructive effect on cardiac tissue by interfering with adducts formed by organelles such as mitochondria (37). In the present study, the expression of 4-HNE was significantly increased in the vehicle + AB group.…”

Section: Discussionsupporting

confidence: 50%

Carnosic acid protects against pressure overload‑induced cardiac remodelling by inhibiting the AKT/GSK3β/NOX4 signalling pathway

Wei

Chen

et al. 2020

Exp Ther Med

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Oxidative stress and apoptosis serve an important role in the development of pressure overload-induced cardiac remodelling. Carnosic acid (CA) has been found to exert antioxidant and anti-apoptotic effects. The present study investigated the underlying mechanism of CA protection and whether this effect was exerted against pressure overload-induced cardiac remodelling. Aortic banding (AB) surgery was performed to induce cardiac remodelling. Mice were randomly divided into four groups (n=15/group): i) Sham + vehicle; ii) sham + CA; iii) AB + vehicle; and iv) AB + CA. After 2 days of AB, 50 mg kg CA was administered orally for 12 days. Echocardiography, histological analysis and molecular biochemistry techniques were performed to evaluate the roles of CA. CA treatment decreased cardiac hypertrophy, fibrosis, oxidative stress and apoptosis in mice challenged with pressure overload. CA also decreased the cross-sectional area of cardiomyocytes and the mRNA and protein expression levels of hypertrophic markers. Furthermore, CA treatment decreased collagen deposition, α-smooth muscle actin expression and the mRNA and protein expression of various fibrotic markers. Additionally, CA reversed the AB-mediated increase in NAPDH oxidase (NOX) 2, NOX4 and 4-hydroxynonenal levels. The number of apoptotic cells was decreased following CA treatment following under conditions of pressure overload. CA also suppressed the activation of AKT and glycogen synthase kinase 3 β (GSK3β) in mice challenged with AB. The present results suggested that CA could inhibit pressure overload-induced cardiac hypertrophy and fibrosis by suppressing the AKT/GSK3β/NOX4 signalling pathway. Therefore, CA may be a promising therapy for cardiac remodelling.

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Section: Discussionsupporting

confidence: 50%

Carnosic acid protects against pressure overload‑induced cardiac remodelling by inhibiting the AKT/GSK3β/NOX4 signalling pathway

Wei

Chen

et al. 2020

Exp Ther Med

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“…In particular, the upregulation in 4-HNE because of oxidative stress was detected in numerous cardiac pathologies (e.g., diabetic cardiomyopathy). 4-HNE damages the myocardium, interfering with mitochondria and making adducts [72]. Previously, we reported not only a decrease in oxidative stress both in the serum and in the liver [33], but also in inflammation both in the brain [26] and in the adipose tissue [34] of HFD fed rats supplemented with tart cherries.…”

Section: Discussionmentioning

confidence: 92%

Anti-Inflammatory and Antioxidant Properties of Tart Cherry Consumption in the Heart of Obese Rats

Martinelli

Tomassoni

Bellitto

et al. 2022

Biology

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Obesity is a risk factor for cardiovascular diseases, frequently related to oxidative stress and inflammation. Dietary antioxidant compounds improve heart health. Here, we estimate the oxidative grade and inflammation in the heart of dietary-induced obese (DIO) rats after exposure to a high-fat diet compared to a standard diet. The effects of tart cherry seed powder and seed powder plus tart cherries juice were explored. Morphological analysis and protein expressions were performed in the heart. The oxidative status was assessed by the measurement of protein oxidation and 4-hydroxynonenal in samples. Immunochemical and Western blot assays were performed to elucidate the involved inflammatory markers as proinflammatory cytokines and cellular adhesion molecules. In the obese rats, cardiomyocyte hypertrophy was accompanied by an increase in oxidative state proteins and lipid peroxidation. However, the intake of tart cherries significantly changed these parameters. An anti-inflammatory effect was raised from tart cherry consumption, as shown by the downregulation of analyzed endothelial cell adhesion molecules and cytokines compared to controls. Tart cherry intake should be recommended as a dietary supplement to prevent or counteract heart injury in obese conditions.

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“…The increase in 4-HNE due to oxidative stress has been observed in several cardiac diseases, for example, diabetic cardiomyopathy. 4-HNE damages the myocardium by interfering with mitochondria and forming adducts [52].…”

Section: Discussionmentioning

confidence: 99%

Cardiovascular Changes Related to Metabolic Syndrome: Evidence in Obese Zucker Rats

Martinelli

Tomassoni

Moruzzi

et al. 2020

IJMS

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Metabolic syndrome (MetS) is a predictor of cardiovascular diseases, commonly associated with oxidative stress and inflammation. However, the pathogenic mechanisms are not yet fully elucidated. The aim of the study is to evaluate the oxidative status and inflammation in the heart of obese Zucker rats (OZRs) and lean Zucker rats (LZRs) at different ages. Morphological and morphometric analyses were performed in the heart. To study the oxidative status, the malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), protein oxidation, and antioxidant enzymes were measured in plasma and heart. To elucidate the inflammatory markers involved, immunohistochemistry and Western blot were performed for cellular adhesion molecules and proinflammatory cytokines. OZRs were characterized by hypertension, hyperlipidemia, hyperglycemia, and insulin resistance. The obesity increased MDA and decreased the activities of superoxide dismutase (SOD) in plasma as well as in the heart, associated with cardiomyocytes hypertrophy. OxyBlot in plasma and in heart showed an increase of oxidativestate proteins in OZRs. Vascular cell adhesion molecule-1, interleukin-6, and tumor necrosis factor-α expressions in OZRs were higher than those of LZRs. However, these processes did not induce apoptosis or necrosis of cardiomyocytes. Thus, MetS induces the lipid peroxidation and decreased antioxidant defense that leads to heart tissue changes and coronary inflammation.

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Increased 4‐hydroxy‐2‐nonenal‐induced proteasome dysfunction is correlated with cardiac damage in streptozotocin‐injected rats with isoproterenol infusion

Cited by 9 publications

References 39 publications

Carnosic acid protects against pressure overload‑induced cardiac remodelling by inhibiting the AKT/GSK3β/NOX4 signalling pathway

Carnosic acid protects against pressure overload‑induced cardiac remodelling by inhibiting the AKT/GSK3β/NOX4 signalling pathway

Anti-Inflammatory and Antioxidant Properties of Tart Cherry Consumption in the Heart of Obese Rats

Cardiovascular Changes Related to Metabolic Syndrome: Evidence in Obese Zucker Rats

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