IncN1 ST7 Epidemic Plasmid Carrying blaIMP-4 in One ST85-Type Klebsiella oxytoca Clinical Isolate with Porin Deficiency

Abstract

Purpose Klebsiella oxytoca is an opportunistic pathogen causing nosocomial infections. This study was designed to characterize the genomic features of a carbapenem-resistant K. oxytoca strain and analyze its molecular characteristics. Materials and Methods The strain wzx-IMP was isolated from the blood of a 2-year-old girl diagnosed with acute myeloid leukemia-M7. Species identification was performed, and the minimal inhibitory concentration of the strain was measured. Multilocus sequence typing was performed to identify the subtypes of K. oxytoca . The transfer capacity of the blaIMP-4-harboring plasmid was investigated by conjugation experiments, and the genome characteristics of the strain were examined using whole-genome sequencing. Results wzx-IMP belongs to the ST85 type and is resistant to imipenem and meropenem, which harbored the blaIMP-4 gene. The blaIMP-4 gene was located in an IS26-associated class 1 integron of pwzx_IMP, which contains conserved IncN1-type backbone regions with a replication gene and its accessory structure for plasmid replication. The blaIMP-4-carrying plasmid in wzx-IMP was successfully transferred to Escherichia coli EC600 by conjugation. Whole-genome sequencing showed that the wzx-IMP isolate included the blaOXY-1-1 gene, accompanied by OmpK36 absence. Conclusion We report an ST85-type carbapenem-resistant K. oxytoca strain, which produces blaIMP-4 located in an IncN1-type plasmid and accompanied by OmpK36 porin deficiency.

Molecular Epidemiology of Extended-Spectrum Beta-Lactamase and AmpC Producing Enterobacteriaceae among Sepsis Patients in Ethiopia: A Prospective Multicenter Study

“…In addition, different intrinsic bla OXY variants encoded with K. oxytoca that are ESBL were detected even though most were found at TASH and DRH, but rarely at HUCSH and Y12HMC. After the first detection of bla OXY in Spain in 2009 [ 37 ], several variants were detected in this study showing how fast the variants are spreading throughout the world [ 38 , 39 ]. In addition to bla GES-11 , the rarely detected ESBL genes that are grouped in class C and has having ESBL activities were bla SRT-1 and bla CMY-42 , and this showed agreements with other studies [ 40 , 41 ].…”

Phenotypic and genomic characteristics of clinical IMP-producing Klebsiella spp. Isolates in China

“…Synthesizing across previous researches, the IncN plasmids were the major carrier of bla IMP gene among Klebsiella spp. and play an important role in transmission of carbapenemases 9 , 13 , 40 . And in our study, the broad-host-range and conjugative IncN plasmid was detected in diverse ST types, and the fusion events between IncN and other types of plasmids including IncR and IncFII plasmids were observed, indicating a potential route for the evolution of IncN plasmids.…”