IncN plasmids carrying blaCTX-M-1 in Escherichia coli isolates on a dairy farm

Dolejská, Monika; Jurcickova, Zuzana; Literák, Ivan; Pokludová, Lucie; Bureš, Jiří; Hera, Alfréd; Kohoutová, L.; Smoła, J.; Čížek, Alois

doi:10.1016/j.vetmic.2010.11.032

Cited by 54 publications

(45 citation statements)

References 13 publications

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“…While the E. coli isolates harboring bla CTX-M in the other herds (12,27). Differences in the environment, management, or antimicrobial use practices that might have resulted in the exchange of a single plasmid among multiple E. coli strains are not clear.…”

Section: Discussionmentioning

confidence: 99%

Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle

Mollenkopf

Weeman

Daniels

et al. 2012

Appl Environ Microbiol

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ABSTRACTblaCTX-Mbeta-lactamases confer resistance to critically important cephalosporin drugs. Recovered from both hospital- and community-acquired infections,blaCTX-Mwas first reported in U.S. livestock in 2010. It has been hypothesized that veterinary use of cephalosporins in livestock populations may lead to the dissemination of beta-lactamase-encoding genes. Therefore, our objectives were to estimate the frequency and distribution of coliform bacteria harboringblaCTX-Min the fecal flora of Ohio dairy cattle populations. In addition, we characterized the CTX-M alleles carried by the isolates, their plasmidic contexts, and the genetic diversity of the bacterial isolates themselves. We also evaluated the association between ceftiofur use and the likelihood of recovering cephalosporinase-producing bacteria. Thirty fresh fecal samples and owner-reported ceftiofur use data were collected from each of 25 Ohio dairy farms. Fecal samples (n= 747) yielded 70blaCTX-M-positiveEscherichia coliisolates from 5/25 herds, 715blaCMY-2E. coliisolates from 25/25 herds, and 274Salmonellaspp. from 20/25 herds. The within-herd prevalence amongblaCTX-M-positive herds ranged from 3.3 to 100% of samples. Multiple pulsed-field gel electrophoresis (PFGE) patterns, plasmid replicon types, and CTX-M genes were detected. Plasmids with CTX-M-1, -15, and -14 alleles were clonal by restriction fragment length polymorphism (RFLP) within herds, and specific plasmid incompatibility group markers were consistently associated with eachblaCTX-Mallele. PFGE of total bacterial DNA showed similar within-herd clustering, with the exception of one herd, which revealed at least 6 different PFGE signatures. We were unable to detect an association between owner-reported ceftiofur use and the probability of recoveringE. colicarryingblaCTX-MorblaCMY-2.

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Section: Discussionmentioning

confidence: 99%

Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle

Mollenkopf

Weeman

Daniels

et al. 2012

Appl Environ Microbiol

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“…The samples from buffered peptone water were enriched in MacConkey broth and subcultivated onto MacConkey agar (MCA) containing cefotaxime (2 mg liter Ϫ1 ) to detect E. coli strains with extended-spectrum beta-lactamase (ESBL) and subsequently onto MCA supplemented with ciprofloxacin (0.06 mg liter Ϫ1 ) to isolate fluoroquinolone-resistant E. coli isolates. These E. coli isolates were further characterized; specifically, the transferabilities of their ESBL and plasmid-encoded quinolone resistance genes was tested by conjugation as described elsewhere previously (1,2). Plasmids of ESBL and quinoloneresistant strains were characterized by replicon typing and EcoRV digestion (1,2).…”

mentioning

confidence: 99%

“…These E. coli isolates were further characterized; specifically, the transferabilities of their ESBL and plasmid-encoded quinolone resistance genes was tested by conjugation as described elsewhere previously (1,2). Plasmids of ESBL and quinoloneresistant strains were characterized by replicon typing and EcoRV digestion (1,2). All E. coli strains were identified by using the API 10S test (bioMérieux, France).…”

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confidence: 99%

Antimicrobial Resistance in Fecal Escherichia coli Isolates from Healthy Urban Children of Two Age Groups in Relation to Their Antibiotic Therapy

Literák

Petro

Dolejská

et al. 2011

Antimicrob Agents Chemother

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The study was performed in the Czech Republic during 2007 to 2009. Of Escherichia coli isolates from 275 children aged 6 weeks, 36% (n ‫؍‬ 177) were resistant to 1 to 7 antibiotics. Of isolates from 253 children aged 6 to 17 years, 24% (n ‫؍‬ 205) were resistant to 1 to 5 antibiotics. There was no significant difference in the prevalences of antibiotic-resistant E. coli isolates between these groups of children, even though the consumptions of antibiotics were quite different.

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“…These overall practices should be discouraged. For instance, the management system accounts for the prevalence of ESBL-producing E. coli in cattle [34]. Moreover, intensive poultry breeding and high concentrations of animals in the same premise favors the transmission of resistant bacteria among animals [35].…”

Section: Antimicrobial Therapy and Emergence Of Esbls In Veterinary Mmentioning

confidence: 99%

An overview of extended-spectrum beta-lactamases in veterinary medicine and their public health consequences

Nobrega

Brocchi

2014

J Infect Dev Ctries

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Serious human and animal infections caused by bacteria are usually treated with beta-lactams. Extended-spectrum beta-lactamases (ESBLs) constitute the most clinically and economically important enzymes that are able to hydrolyze and inactivate beta-lactam antibiotics in veterinary medicine. The spread of ESBLs represents a serious threat to healthcare systems, drastically undermining therapeutic options. The relationship between drug usage and the emergence of resistance has been extensively reported. Nevertheless, the use of antimicrobials in veterinary medicine and the emergence of ESBLs in animals remains a matter of debate. Moreover, there is still controversy about whether antibiotic usage in farm animals poses a potential public health risk. This review will (i) deal with aspects related to the presence of ESBLs in veterinary medicine, (ii) its link with human medicine, and (iii) discuss strategies to be implemented to preserve antimicrobial effectiveness. New insights relative to old questions concerning antimicrobial use in domestic animals are also presented.

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IncN plasmids carrying blaCTX-M-1 in Escherichia coli isolates on a dairy farm

Cited by 54 publications

References 13 publications

Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle

Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle

Antimicrobial Resistance in Fecal Escherichia coli Isolates from Healthy Urban Children of Two Age Groups in Relation to Their Antibiotic Therapy

An overview of extended-spectrum beta-lactamases in veterinary medicine and their public health consequences

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