Incidence of Shiga toxin-producing Escherichia coli serogroups in ruminant's meat

Momtaz, Hassan; Dehkordi, Farhad Safarpoor; Rahimi, Ebrahim; Ezadi, Hossein; Arab, Reza

doi:10.1016/j.meatsci.2013.04.051

Cited by 80 publications

(99 citation statements)

References 52 publications

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“…They represented that 54.54% of E. coli isolates were STEC. In addition to our results and also finding of Momtaz et al, 13 Hemmatinezhad et al 14 and Ranjbar et al, 15 simultaneous presences of stx1, stx2, eaeA and ehly virulence factors have also been reported by Franz et al, 17 JayRussell et al 18 and Kabiru et al 19 …”

Section: Discussionsupporting

confidence: 89%

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Shiga-toxigenic Escherichia coli in ready-to-eat food staffs: Prevalence and distribution of putative virulence factors

Shahreza

Rahimi

Momtaz

2017

Microbiol Res (Pavia)

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Lack of proper hygiene and using from low quality raw materials cause high presence of food-borne pathogens in ready to eat foods. Shiga toxin producing Escherichia coli is one of the most common cause of food-borne diseases in the world. The present research was done to study the prevalence and distribution of virulence factors in the STEC strains isolated from various types of ready to eat food samples. Seven-hundred and twenty food samples were collected and cultured. Isolated E. coli bacteria were approved another time using the 16S rRNA-based PCR amplification. Approved strains were subjected to multiplex PCR for identification of putative virulence factors. Twenty-six out of 720 food samples (5.20%) were positive for E. coli. Salad (15%), candy (12.50%) and barbecue (10%) were the most commonly contaminated. Prevalence of STEC strains was 2.63%. Prevalence of EHEC and AEEC subtypes were 36.84% and 52.63%, respectively. EHEC strains harbored all three stx1, eae and ehly genes. High presence of EHEC strains besides the considerable distribution of multiple virulence factors showed an important public health issue regarding the consumption of ready to eat foods.

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Section: Discussionsupporting

confidence: 89%

“…[13][14][15][16] Momtaz et al 13 reported that the prevalence of E. coli strains among meat samples was 29.02%. They showed a considerable prevalence of E. coli in sheep meat (35.45%).…”

Section: Discussionmentioning

confidence: 99%

Shiga-toxigenic Escherichia coli in ready-to-eat food staffs: Prevalence and distribution of putative virulence factors

Shahreza

Rahimi

Momtaz

2017

Microbiol Res (Pavia)

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“…E. coli is a gram-negative, non-sporulating, rod-shaped, facultative anaerobe and Shiga toxin-producing E. coli (STEC) is a subdivision of enterohemorrhagic E. coli (EHEC) [9,10,11]. Outbreak of food poisoning and foodborne diseases are associated with certain STEC O-serogroups and O157 is the most important serogroup associated with intensive clinical syndromes like lethal hemolytic uremic syndrome (HUS), bloody and non-bloody diarrhea, thrombotic thrombocytopenic purpura (TTP) and hemorrhagic colitis (HC) [9,10,11]. Human infection with E. coli O157:H7 serotype has been associated with contaminated food samples, water, and person-to person transmission [9,10,11].…”

Section: Introductionmentioning

confidence: 99%

“…Outbreak of food poisoning and foodborne diseases are associated with certain STEC O-serogroups and O157 is the most important serogroup associated with intensive clinical syndromes like lethal hemolytic uremic syndrome (HUS), bloody and non-bloody diarrhea, thrombotic thrombocytopenic purpura (TTP) and hemorrhagic colitis (HC) [9,10,11]. Human infection with E. coli O157:H7 serotype has been associated with contaminated food samples, water, and person-to person transmission [9,10,11]. Meats from ruminants and poultry are considered to be the primary reservoirs of E. coli O157:H7 [9,10,11].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> O157:H7 Isolated from Raw Meat Samples of Ruminants and Poultry

Mashak¹

2018

JFNR

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Escherichia coli O157:H7 is one of the most dangerous zoonotic pathogens of meat. The present investigation was done to study the prevalence and antibiotic resistance of E. coli O157:H7 strains isolated from raw meat samples. A total of 458 meat samples were collected. Samples were cultured using what? After which sorbitol negative isolates were analyzed for rfbO157 and flicH7 genes. Thirty-six out of 458 meat samples were positive for sorbitol negative strains of E. coli (7.86%). All of the sorbitol negative strains were also positive for rfbO157 and flicH7 genes. Prevalence of E. coli O157:H7 strains were 7.86%. The prevalence of E. coli O157:H7 in chicken meat was higher than the other samples (16.25%). The genes that encode resistance to ampicillin (CITM) (100%), gentamicin (aac(3)-IV) (94.44%) and tetracycline (tetA) (61.11%) had the highest prevalence. Escherichia coli O157:H7 strains from raw meat samples from ruminants and poultry had the highest resistance to ampicillin (100%), tetracycline (83.33%) and gentamicin (83.33%) respectively. Strains of antibiotic resistant E. coli 0157:H7 found in this present study are of public health importance.

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“…They can be broadly grouped into O157 STEC and non-O157 STEC strains. The pathogenic capability of STEC is primarily based on the possession of virulence factors including Shiga toxins (Stx1 and Stx2) [2] and genes encoding the attaching and effacing function (eae) [2,3]. E. O157:H7 is the STEC species commonly associated with foodborne disease in humans.…”

Section: Introductionmentioning

confidence: 99%

Shiga toxin-producing E. coli isolated from sheep in Namibia

Madzingira¹

2016

J Infect Dev Ctries

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Introduction: Shiga toxin-producing Escherichia coli (STEC) are an important group of emerging zoonotic pathogens carried in the intestinal tracts of ruminants. They can cause mild diarrhea and fatal disease characterized by hemolytic uremic syndrome, especially in children, the elderly, and immune-compromised individuals. Methodology: The aim of this study was to determine if sheep harbor STEC. Sheep feces (n = 40), brisket wool (n = 40), and 150 meat samples were collected from the flank (n = 35), rump (n = 35), brisket (n = 20), shank (n = 25), diaphragm (n = 10), and neck (n = 25) of slaughter-age sheep at a high-throughput abattoir and tested for STEC using a combination of culture and real-time polymerase chain reaction techniques. Results: E. coli O103 (5/40) and O145 (5/40) strains were isolated from the feces and E. coli O157:H7 was isolated from brisket wool (10/40) and flank meat (5/35). The results of this study provide the first report of STEC infections in sheep in Namibia. Conclusions: The results of this study show that sheep, like cattle, can shed STEC strains in their feces, which can contaminate meat and expose humans to infections.

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Incidence of Shiga toxin-producing Escherichia coli serogroups in ruminant's meat

Cited by 80 publications

References 52 publications

Shiga-toxigenic Escherichia coli in ready-to-eat food staffs: Prevalence and distribution of putative virulence factors

Shiga-toxigenic Escherichia coli in ready-to-eat food staffs: Prevalence and distribution of putative virulence factors

Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> O157:H7 Isolated from Raw Meat Samples of Ruminants and Poultry

Shiga toxin-producing E. coli isolated from sheep in Namibia

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