Inactivation of the LysR regulator Cj1000 of Campylobacter jejuni affects host colonization and respiration

Dufour, Virginie; Li, Jennifer; Flint, Annika; Rosenfeld, Eric; Rivoal, Katell; Georgeault, Sylvie; Alazzam, Bachar; Ermel, Gwennola; Stintzi, Alain; Bonnaure‐Mallet, Martine; Baysse, Christine

doi:10.1099/mic.0.062992-0

Cited by 24 publications

(19 citation statements)

References 51 publications

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“…Regulation of metabolic pathways has been demonstrated to be an important stress response of pathogenic bacteria, and a variety of transcriptional regulators such as LysR, RutR and Atf1 have been shown to play central roles during this process3031323334. For example, LysR was shown to be an important regulatory component of P. aeruginosa adaptation during oxidative stress35.…”

Section: Discussionmentioning

confidence: 99%

Genomic and transcriptomic analysis of NDM-1 Klebsiella pneumoniae in spaceflight reveal mechanisms underlying environmental adaptability

Liu

Wang

et al. 2014

Sci Rep

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The emergence and rapid spread of New Delhi Metallo-beta-lactamase-1 (NDM-1)-producing Klebsiella pneumoniae strains has caused a great concern worldwide. To better understand the mechanisms underlying environmental adaptation of those highly drug-resistant K. pneumoniae strains, we took advantage of the China's Shenzhou 10 spacecraft mission to conduct comparative genomic and transcriptomic analysis of a NDM-1 K. pneumoniae strain (ATCC BAA-2146) being cultivated under different conditions. The samples were recovered from semisolid medium placed on the ground (D strain), in simulated space condition (M strain), or in Shenzhou 10 spacecraft (T strain) for analysis. Our data revealed multiple variations underlying pathogen adaptation into different environments in terms of changes in morphology, H2O2 tolerance and biofilm formation ability, genomic stability and regulation of metabolic pathways. Additionally, we found a few non-coding RNAs to be differentially regulated. The results are helpful for better understanding the adaptive mechanisms of drug-resistant bacterial pathogens.

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Section: Discussionmentioning

confidence: 99%

Genomic and transcriptomic analysis of NDM-1 Klebsiella pneumoniae in spaceflight reveal mechanisms underlying environmental adaptability

Liu

Wang

et al. 2014

Sci Rep

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“…Furthermore, expression of katA within C. jejuni is regulated primarily by Fur (15) and PerR (26). However, more recently, additional transcriptional regulators of katA expression have been identified, including Cj1000 (27) and CosR (28), which add additional layers of complexity to katA regulation. In this report, we further contribute to the wealth of knowledge on C. jejuni KatA by providing important insight into a key protein required for trafficking hemin to KatA.…”

Section: Discussionmentioning

confidence: 99%

Cj1386, an Atypical Hemin-Binding Protein, Mediates Hemin Trafficking to KatA in Campylobacter jejuni

Flint

Stintzi

2015

J Bacteriol

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. While the structure and biochemical properties of catalase enzymes have been well characterized over many decades of research, it remained unclear how catalases acquire hemin. We have previously reported that Cj1386 is essential for ensuring proper hemin content in Campylobacter jejuni catalase (KatA) (A. Flint, Y. Q. Sun, and A. Stintzi, J Bacteriol 194:334 -345, 2012). In this report, an in-depth molecular characterization of Cj1386 was performed to elucidate the mechanistic details of this association. Coimmunoprecipitation assays revealed that KatA-Cj1386 transiently interact in vivo, and UV-visible spectroscopy demonstrated that purified Cj1386 protein binds hemin. Furthermore, hemin titration experiments determined that hemin binds to Cj1386 in a 1:1 ratio with hexacoordinate hemin binding. Mutagenesis of potential hemin-coordinating residues in Cj1386 showed that tyrosine 57 was essential for hemin coordination when Cj1386 was overexpressed in Escherichia coli. The importance of tyrosine 57 in hemin trafficking in vivo was confirmed by introducing the cj1386 Y57A allele into a C. jejuni ⌬cj1386 mutant background. The cj1386 Y57A mutation resulted in increased sensitivity toward H 2 O 2 relative to the wild type, suggesting that KatA was not functional in this strain. In support of this finding, KatA immunoprecipitated from the ⌬cj1386؉cj1386 Y57A mutant had significantly reduced hemin content compared to that of the cj1386 WT background. Overall, these findings indicate that Cj1386 is involved in directly trafficking hemin to KatA and that tyrosine 57 plays a key role in this function.

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“…Microarray analysis of the cj1000 mutant revealed several targets, including katA, ahpC and trxB. Although the cj1000 mutant did not show lower viability under oxidative stress, it had attenuated ability to colonise and was outcompeted by the wild-type strain during colonisation of the piglet intestine (Dufour et al 2013).…”

Section: Other Regulatorsmentioning

confidence: 95%

“…Campylobacter jejuni cj1000 encodes a LysR-type regulator that controls O 2 consumption (Dufour et al 2013). Microarray analysis of the cj1000 mutant revealed several targets, including katA, ahpC and trxB.…”

Section: Other Regulatorsmentioning

confidence: 99%

Oxidative and nitrosative stress defences ofHelicobacterandCampylobacterspecies that counteract mammalian immunity

Flint

Stintzi

Saraiva

2016

FEMS Microbiology Reviews

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Helicobacter and Campylobacter species are Gram-negative microaerophilic host-associated heterotrophic bacteria that invade the digestive tract of humans and animals. Campylobacter jejuni is the major worldwide cause of foodborne gastroenteritis in humans, while Helicobacter pylori is ubiquitous in over half of the world's population causing gastric and duodenal ulcers. The colonisation of the gastrointestinal system by Helicobacter and Campylobacter relies on numerous cellular defences to sense the host environment and respond to adverse conditions, including those imposed by the host immunity. An important antimicrobial tool of the mammalian innate immune system is the generation of harmful oxidative and nitrosative stresses to which pathogens are exposed during phagocytosis. This review summarises the regulators, detoxifying enzymes and subversion mechanisms of Helicobacter and Campylobacter that ultimately promote the successful infection of humans.

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Inactivation of the LysR regulator Cj1000 of Campylobacter jejuni affects host colonization and respiration

Cited by 24 publications

References 51 publications

Genomic and transcriptomic analysis of NDM-1 Klebsiella pneumoniae in spaceflight reveal mechanisms underlying environmental adaptability

Genomic and transcriptomic analysis of NDM-1 Klebsiella pneumoniae in spaceflight reveal mechanisms underlying environmental adaptability

Cj1386, an Atypical Hemin-Binding Protein, Mediates Hemin Trafficking to KatA in Campylobacter jejuni

Oxidative and nitrosative stress defences ofHelicobacterandCampylobacterspecies that counteract mammalian immunity

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