2002
DOI: 10.1128/aem.68.10.4894-4899.2002
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Abstract: The mosquitocidal toxin (MTX) produced by Bacillus sphaericus strain SSII-1 is an ϳ97-kDa single-chain toxin which contains a 27-kDa enzyme domain harboring ADP-ribosyltransferase activity and a 70-kDa putative binding domain. Due to cytotoxicity toward bacterial cells, the 27-kDa enzyme fragment cannot be produced in Escherichia coli expression systems. However, a nontoxic 32-kDa N-terminal truncation of MTX can be expressed in E. coli and subsequently cleaved to an active 27-kDa enzyme fragment. In vitro the… Show more

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Cited by 32 publications
(17 citation statements)
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“…13,18 However, the expression is reasonable when MTX 30-264 is extended by the inhibitory linker to the ricin B-like domains. The expression level is improved further by applying the mutations E195Q or E197Q that destroy the catalytically crucial E-x-E sequence motif of this ADP-ribosyl transferase.…”
Section: Resultsmentioning
confidence: 98%
“…13,18 However, the expression is reasonable when MTX 30-264 is extended by the inhibitory linker to the ricin B-like domains. The expression level is improved further by applying the mutations E195Q or E197Q that destroy the catalytically crucial E-x-E sequence motif of this ADP-ribosyl transferase.…”
Section: Resultsmentioning
confidence: 98%
“…The activation loop in the crystal structure is exposed and would allow proteolytic cleavage to allow the lectin domain to mediate uptake of the catalytic domain into the target cell in a manner typical of this class of toxin. Although the target(s) of Mtx1 in mosquito cells are unknown, Mtx1 modifies unknown target proteins in Culex quinquefasciatus (Thanabalu et al, 1993), several proteins in HeLa cells, EF-Tu in E. coli (Schirmer et al, 2002b) and soybean trypsin inhibitor, apparently targeting arginine residues for modification (Carpusca et al, 2006;Schirmer et al, 2002a). However, the kinetics of this reaction with respect to (Treiber et al, 2008).…”
Section: Mtx1mentioning
confidence: 91%
“…16,17 The catalytic domain of MTX ADP-ribosylates arginines of various proteins. 18,19 However, a specific in vivo target in mosquito larvae has not yet been detected. Based on a sequence comparison, the C-terminal residues have been related to ricin B.…”
Section: Introductionmentioning
confidence: 98%