Inactivation of H+-ATPase Participates in the Influence of Variation Potential on Photosynthesis and Respiration in Peas

Abstract

Local damage (e.g., burning, heating, or crushing) causes the generation and propagation of a variation potential (VP), which is a unique electrical signal in higher plants. A VP influences numerous physiological processes, with photosynthesis and respiration being important targets. VP generation is based on transient inactivation of H+-ATPase in plasma membrane. In this work, we investigated the participation of this inactivation in the development of VP-induced photosynthetic and respiratory responses. Two- to three-week-old pea seedlings (Pisum sativum L.) and their protoplasts were investigated. Photosynthesis and respiration in intact seedlings were measured using a GFS-3000 gas analyzer, Dual-PAM-100 Pulse-Amplitude-Modulation (PAM)-fluorometer, and a Dual-PAM gas-exchange Cuvette 3010-Dual. Electrical activity was measured using extracellular electrodes. The parameters of photosynthetic light reactions in protoplasts were measured using the Dual-PAM-100; photosynthesis- and respiration-related changes in O2 exchange rate were measured using an Oxygraph Plus System. We found that preliminary changes in the activity of H+-ATPase in the plasma membrane (its inactivation by sodium orthovanadate or activation by fusicoccin) influenced the amplitudes and magnitudes of VP-induced photosynthetic and respiratory responses in intact seedlings. Decreases in H+-ATPase activity (sodium orthovanadate treatment) induced fast decreases in photosynthetic activity and increases in respiration in protoplasts. Thus, our results support the effect of H+-ATPase inactivation on VP-induced photosynthetic and respiratory responses.

A Theoretical Analysis of Relations between Pressure Changes along Xylem Vessels and Propagation of Variation Potential in Higher Plants

“…Using Equations (5), (6), and 7, we derived Equations (11), (12), and (13) for describing the dependence of tth on distance from the damaged zone (x) in V1, V2, and V3, respectively.…”

“…Using Equations (11), (12), and (13), we derived Equations (16), (17), and (18) for describing the dependence of the rate of VP propagation (VVP) on the distance from the damaged zone (x) in V1, V2, and V3, respectively. These equations were based on a description of VVP as dx/dtth.…”

“…[ 5 ]. It is mainly related to the activation of Ca 2+ channels and the inactivation of H + -ATPase [ 3 , 5 , 11 ]; however, the transient activation of other anion channels can also participate in VP generation [ 5 , 7 ].…”

Influence of Local Burning on Difference Reflectance Indices Based on 400–700 nm Wavelengths in Leaves of Pea Seedlings

“…Photosynthesis is an important target of ESs [ 5 ]. It is known that ESs decrease photosynthetic CO 2 assimilation, quantum yield of photosystem I, quantum yield of photosystem II (Y(II)), and mesophyll CO 2 conductance [ 23 , 27 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ]. Non-photochemical quenching of chlorophyll fluorescence (NPQ), cyclic electron flow, and photosynthetic light absorption can be stimulated by ESs [ 27 , 37 , 39 , 43 , 44 , 45 ].…”

“…It is known that ESs decrease photosynthetic CO 2 assimilation, quantum yield of photosystem I, quantum yield of photosystem II (Y(II)), and mesophyll CO 2 conductance [ 23 , 27 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ]. Non-photochemical quenching of chlorophyll fluorescence (NPQ), cyclic electron flow, and photosynthetic light absorption can be stimulated by ESs [ 27 , 37 , 39 , 43 , 44 , 45 ]. Increasing plant tolerance to stressors (including tolerance of photosynthetic machinery) is likely the result of physiological changes [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ].…”