Freezing is a major environmental stress, which limits plant’s distribution, growth and productivity. Ice nucleation active bacteria can catalyze ice formation at temperatures as high as −2°C. A membrane protein confer the ability of ice nucleation, called ice-nucleating proteins (INPs), which is encoded by a single gene. Mutation in this gene will lead to delaying of ice nucleation. In this study, leaf tissues of several plants with freezing symptoms were collected from different locations and 40 bac…
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