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Abstract: In vivo phosphorylation of the five histone H1 variants H1a-H1e including H1(0) in NIH 3T3 mouse fibroblasts was examined during the cell cycle by using a combination of HPLC techniques and conventional AU gel electrophoresis. Phosphorylation starts during the late G1 phase and increases throughout the S phase. In the late S phase, the H1 variants exist as a combination of molecules containing 0 or 1 (H1a, H1c), 0-2 (H1d), or 0-3 (H1b, H1e) phosphate groups with a share of unphosphorylated protein ranging betw… Show more

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Cited by 87 publications
(85 citation statements)
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References 33 publications
(47 reference statements)
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“…5). In growth-arrested cells at G 1 phase, linker histones are unphosphorylated, whereas in late S phase, the H1 variants exist as a combination of molecules containing one to three phosphate groups, according to the particular subtype (15). The fastest migrating components in capillary electrophoresis are the unphosphorylated H1 histones (29,36) (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…The presence of this large number of various H1 histone subtypes and their possible posttranslational modifications, such as phosphorylation (15), make it very probable that H1 histones play numerous structural and functional roles in chromatin. Until now, no specific role for the various variants has been established although Kaludov et al (16) showed that the mouse histone H1b binds preferentially to a regulatory sequence within a mouse H3.2 replication-dependent histone gene.…”
mentioning
confidence: 99%
“…For isolation of histones, nuclei from NIH fibroblasts were prepared as described. 13 H1 histones were isolated from the resulting nuclear preparations by extracting with 5% perchloric acid at 48C for 1 h. The mixture was centrifuged at 10 0006g for 20 min. The remaining pellet was resuspended with 0.4 M H 2 SO 4 at 48C for 1 h to extract the core histones.…”
Section: Labeling Conditions and Isolation Of H1 And Core Histonesmentioning
confidence: 99%
“…12 The cdc 2/H1 kinase-dependent phosphorylation of H1 histones during the cell cycle is a dynamic process with a phosphorylation maximum during mitosis. 13 Whereas H2A histones are constantly phosphorylated, phosphorylation of H4 and H2B histones is negligible throughout the cell cycle. Histone H3 phosphorylation, in particular, appears to be essential for proper mitotic chromosome condensation and segregation, 14,15 but was also shown to be involved in premature chromosome condensation.…”
Section: Introductionmentioning
confidence: 99%
“…H1 histones are phosphorylated at multiple sites in the N-and C-terminal tails, in a cell cycle dependent manner. During G1 phase, H1 phosphorylation is low, while addition of phosphate groups increases during the S-and G2-phases to reach maximum at the M-phase (17). This is executed by one or more kinases, for example, CDK2 (18).…”
mentioning
confidence: 99%