In Vivo Optogenetic Modulation with Simultaneous Neural Detection Using Microelectrode Array Integrated with Optical Fiber

Abstract

The detection of neuroelectrophysiology while performing optogenetic modulation can provide more reliable and useful information for neural research. In this study, an optical fiber and a microelectrode array were integrated through hot-melt adhesive bonding, which combined optogenetics and electrophysiological detection technology to achieve neuromodulation and neuronal activity recording. We carried out the experiments on the activation and electrophysiological detection of infected neurons at the depth range of 900–1250 μm in the brain which covers hippocampal CA1 and a part of the upper cortical area, analyzed a possible local inhibition circuit by combining opotogenetic modulation and electrophysiological characteristics and explored the effects of different optical patterns and light powers on the neuromodulation. It was found that optogenetics, combined with neural recording technology, could provide more information and ideas for neural circuit recognition. In this study, the optical stimulation with low frequency and large duty cycle induces more intense neuronal activity and larger light power induced more action potentials of neurons within a certain power range (1.032 mW–1.584 mW). The present study provided an efficient method for the detection and modulation of neurons in vivo and an effective tool to study neural circuit in the brain.

Electrodeposited NaYF4:Yb3+, Er3+ up-conversion films for flexible neural device construction and near-infrared optogenetics

“…In addition, traditional microelectrode probes are usually based on rigid materials, such as silicon and metal, resulting in a significant mechanical mismatch between rigid probes and soft brain tissues. 15–18 The mechanical mismatch between the probes and tissues has been shown to induce chronic inflammatory responses, resulting in glial scar formation and signal degradation over the long term. 19–22 Thus, it is highly desirable to develop flexible devices that can enable simultaneous optogenetic stimulation and electrophysiological recording in a minimally invasive manner.…”

Implantable Optrode Array for Optogenetic Modulation and Electrical Neural Recording

“…An integrated device that can simultaneously deliver light stimuli to activate or inhibit specific neuronal cells and record the neural response activities has been highly demanded [9,[11][12][13][14][15][16][17][18]. As this micro-electro-mechanical systems (MEMS) technology advances, optical stimulation and recording system configuration can be fabricated on a microscale [19][20][21][22][23][24][25]. Therefore, a MEMS-based optrode is considered to be one of the promising optogenetic platforms.…”