In vivo monitoring of neural stem cells after transplantation in acute cerebral infarction with dual-modal MR imaging and optical imaging

Wen, Xuehua; Wang, Yong; Zhang, Fang; Zhang, Xiang; Lu, Liejing; Shuai, Xintao; Shen, Jun

doi:10.1016/j.biomaterials.2014.02.042

Cited by 67 publications

(63 citation statements)

References 32 publications

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“…27 MRI has been the most widely investigated imaging modality for in vivo tracking of the biodistribution and migration of stem cells in a stroke. [9][10][11]23 In our study, MSCs were labeled by using SPION-loaded cationic polymersomes with a relatively low SPION concentration and an obviously short labeling period. This labeling had no adverse effects on cell viability, apoptosis and oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…21,23 Two days after MCAO, animals were randomly selected to receive stereotactic injection of 5×10 5 GFP-MSCs pre-labeled with SPION-loaded polymersomes, unlabeled GFP-MSCs, or phosphate-buffered saline (PBS; n=18 in each group) into the right striatum (stereotaxic coordinates: 3.0 mm lateral to bregma, 0.5 mm rostral to bregma, and 6.0 mm deep from the pial surface) using a 26 s gauge needle attached to a 25-µL Hamilton syringe mounted on a microinjector. Before the injection, GFP-MSCs were suspended in 3 µL PBS, and cell viability was determined to be greater than 90%.…”

Section: Animal Model and Cell Transplantationmentioning

confidence: 99%

“…The detailed acquisition parameters and derivation of T2 relaxation times from T2-mapping were described previously. 21,23 In vivo MRI was performed on a clinical 3.0-T system (Achieva; Philips Medical Systems) with a 50×50 mm 4-channel phased array rat coil (Shanghai Chenguang Medical Technologies, People's Republic of China) at 1, 2, 3, 4, 6, and 8 weeks after transplantation. The acquisition sequences included axial and coronal FSE T2WI (TR/ TE =800/60 ms; NSA =2) and FFE T2*WI (TR/TE =500/18 ms; Flip angel =20°; NSA =3).…”

Section: Mrimentioning

confidence: 99%

“…Slice hyperintense areas were then summated to generate infarct volume as a percentage of contralateral intact brain volume. 21,23 histology Immediately after MRI, three rats in each group were randomly sacrificed by anesthetic overdose, then transcardially perfused with saline and, further, with 4% paraformaldehyde in PBS at 2, 4, and 8 weeks after cells transplantation. Brains were harvested and post-fixed for 1-2 h and then cryoprotected in 20% sucrose solution.…”

Section: Mrimentioning

confidence: 99%

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The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia

Duan

Wang

et al. 2017

IJN

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Understanding the long-term fate and potential mechanisms of mesenchymal stem cells (MSCs) after transplantation is essential for improving functional benefits of stem cell-based stroke treatment. Magnetic resonance imaging (MRI) is considered an attractive and clinically translatable tool for longitudinal tracking of stem cells, but certain controversies have arisen in this regard. In this study, we used SPION-loaded cationic polymersomes to label green fluorescent protein (GFP)-expressing MSCs to determine whether MRI can accurately reflect survival, long-term fate, and potential mechanisms of MSCs in ischemic stroke therapy. Our results showed that MSCs could improve the functional outcome and reduce the infarct volume of stroke in the brain. In vivo MRI can verify the biodistribution and migration of grafted cells when pre-labeled with SPION-loaded polymersome. The dynamic change of low signal volume on MRI can reflect the tendency of cell survival and apoptosis, but may overestimate long-term survival owing to the presence of iron-laden macrophages around cell graft. Only a small fraction of grafted cells survived up to 8 weeks after transplantation. A minority of these surviving cells were differentiated into astrocytes, but not into neurons. MSCs might exert their therapeutic effect via secreting paracrine factors rather than directing cell replacement through differentiation into neuronal and/or glial phenotypes.

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Section: Discussionmentioning

confidence: 99%

Section: Animal Model and Cell Transplantationmentioning

confidence: 99%

Section: Mrimentioning

confidence: 99%

Section: Mrimentioning

confidence: 99%

See 2 more Smart Citations

The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia

Duan

Wang

et al. 2017

IJN

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“…As a solution to the calibration and tracking of the MSC number after systemic injection, monitoring of the stem cells has been proposed before injection [59].…”

Section: Delivery Methods Of Mscs In Burn Wound Studymentioning

confidence: 99%

Experimental stem cell therapies on burn wound: Do source, dose, timing and method matter?

Öztürk

Karagöz

2015

Burns

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Polymersomes: Synthesis and Applications

Poma

Pei

Ruiz‐Pérez

et al. 2018

Encyclopedia of Polymer Science and Technology

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Compartmentalization, that is the separation into discrete compartments deputed to absolve precise functions, is paramount for most biological systems. Natural cells possess suitable structures called membranes, formed by amphiphilic molecules. In an effort to mimic and improve these structures and exploit them for research, diagnostic and therapeutic purposes, about 20 years ago the polymersome technology was born. Polymersomes are self‐assembled structures formed by synthetic amphiphilic block copolymers. Their physicochemical characteristics make them extremely attractive nanosystems for loading and delivering all sorts of cargos, ranging from small drug molecules and reporters to proteins to DNA, all the way up to the actual generation of artificial cell organelles. In this chapter, we will discuss different aspects related to this technology, ranging from synthetic polymer chemistry up to polymersome production and cargo encapsulation approaches, and eventually concluding with their physicochemical behavior and latest biological applications.

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In vivo monitoring of neural stem cells after transplantation in acute cerebral infarction with dual-modal MR imaging and optical imaging

Cited by 67 publications

References 32 publications

The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia

The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia

Experimental stem cell therapies on burn wound: Do source, dose, timing and method matter?

Polymersomes: Synthesis and Applications

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