In vivo monitoring of glial scar proliferation on chronically implanted neural electrodes by fiber optical coherence tomography

Xie, Yisong; Martini, Nadja; Hassler, Christina; Kirch, Robert D.; Seifert, Andreas; Hofmann, Ulrich G.

doi:10.3389/fneng.2014.00034

Cited by 48 publications

(57 citation statements)

References 33 publications

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“…Successful attempts have been made in studying brain tissue in vivo by several groups. (17,55,56) So far, however, these protocols are applicable for animals only. In the same way that RF lesioning can be combined with optics, it may be possible that intraoperative MER and local field potential measurements, commonly used during DBS implantation, could be used together as a multiparametric tool.…”

Section: Discussionmentioning

confidence: 99%

Optical Monitoring Techniques for Navigation during Stereotactic Neurosurgery

2016

Sensors and Materials

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Optical techniques are becoming increasingly important in healthcare, as both therapeutic and diagnostic tools. In neurosurgery, photonic technologies such as the microscope have already found widespread use. The overall aim of this paper is to give an overview of optical monitoring methods and their implementation and use in stereotactic and functional neurosurgery. The development steps presented in this paper range from radiofrequency-lesion size estimation using optics to a system adapted for neurosurgical intraoperative navigation during stereotactic deep brain stimulation (DBS) lead implantations. The two main optical methods implemented as intraoperative guidance tools are laser Doppler flowmetry and diffuse reflectance spectroscopy. By combining these fundamental methods with probe designs adapted to stereotactic systems, vessels can be tracked and "bar-codes" of tissue type along pre-defined trajectories used for DBS implantations identified. Examples of the optical methods used in relation to stereotactic DBS implantations are given.

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Section: Discussionmentioning

confidence: 99%

Optical Monitoring Techniques for Navigation during Stereotactic Neurosurgery

2016

Sensors and Materials

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“…To investigate the effect of plasma deposited CH 4 coatings on glial scar development around OCT probes, OCT probes were either CH 4 -coated or uncoated. Then, tissue properties were assessed by measuring the tissue attenuation coefficient of the OCT signal (Xie et al, , 2014 and finally by IHC.…”

Section: Methodsmentioning

confidence: 99%

“…The OCT imaging system used in this study is identical to that which is previously described (Xie et al, , 2014.…”

Section: Optical Coherence Tomographymentioning

confidence: 99%

“…Here we report our approach using fiber-based optical coherence tomography to monitor the development of the glial scar in-vivo over the course of the implantation. We previously demonstrated the use of fiber-based OCT to gain insight as a minimally-invasive imaging modality during in-vivo whole rat brain trajectories and flexible implants (Xie et al, 2014), and elucidated the bases of its contrast's origins . In this study, we first sought to compare the ability of fiber-based OCT with traditional IHC in observing the glial scar around another rigid implanted device (i.e.…”

Section: Introductionmentioning

confidence: 99%

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Long-term in vivo Monitoring of Gliotic Sheathing of Ultrathin Entropic Coated Brain Microprobes with Fiber-based Optical Coherence Tomography

Dryg

Xie

Bergmann

et al. 2020

Preprint

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Microfabricated neuroprosthetic devices have made possible important observations on neuron activity; however, long-term high-fidelity recording performance of these devices has yet to be realized. Tissue-device interactions appear to be a primary source of lost recording performance. The current state of the art for visualizing the tissue response surrounding brain implants in animals is Immunohistochemistry + Confocal Microscopy, which is mainly performed after sacrificing the animal. Monitoring the tissue response as it develops could reveal important features of the response which may inform improvements in electrode design. Optical Coherence Tomography (OCT), an imaging technique commonly used in ophthalmology, has already been adapted for imaging of brain tissue. Here, we use OCT to achieve real-time, in vivo monitoring of the tissue response surrounding chronically implanted neural devices. The employed tissueresponse-provoking implants are coated with a plasma-deposited nanofilms, which have been demonstrated as a biocompatible and anti-inflammatory interface for indwelling devices. We evaluate the method by comparing the OCT results to traditional histology qualitatively and quantitatively. The differences in OCT signal across the implantation period between the plasma group and the control reveal that the Parylene-type coating of otherwise rigid brain probes (glass and silicon) does not improve the glial encapsulation in the brain parenchyma.

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“…Particularly for optogenetics applications, glial encapsulation can increase the backscattering and attenuate light delivered to host tissue 126 .…”

Section: Materials Long-term Compatibility and Safetymentioning

confidence: 99%

Miniaturized optogenetic neural implants: a review

Fan

2015

Lab Chip

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Optogenetics is an exciting new technology that allows targetable fast control and readout of specific neural populations in complex brain circuits. With the rapid development of light-sensitive microbial opsins, substantial gains in understanding the causal relationships between neural activity and behavior in both healthy and diseased brains have been achieved during the last decade. However, the intricate and complex interactions between different neural populations in mammalian brains require novel, implantable, neural interfaces that are capable of manipulating and probing targeted neurons at multiple sites and with high spatiotemporal resolution. Advanced microtechnology has offered the highest potential to meet these demands of optogenetic applications. In this paper, we review a variety of miniaturized optogenetic neural implants developed in recent years, based on different light sources, including lasers, laser diodes, and light-emitting diodes. We then summarize the specifications of these microimplants and their related microfabrication approaches and discuss the major challenges of current techniques and the vision for the future of the field.

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In vivo monitoring of glial scar proliferation on chronically implanted neural electrodes by fiber optical coherence tomography

Cited by 48 publications

References 33 publications

Optical Monitoring Techniques for Navigation during Stereotactic Neurosurgery

Optical Monitoring Techniques for Navigation during Stereotactic Neurosurgery

Long-term in vivo Monitoring of Gliotic Sheathing of Ultrathin Entropic Coated Brain Microprobes with Fiber-based Optical Coherence Tomography

Miniaturized optogenetic neural implants: a review

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