In vivo fluorescence of human skin. A potential marker of photoaging

Leffell, D. J.

doi:10.1001/archderm.124.10.1514

Cited by 52 publications

(56 citation statements)

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“…In vivo fluorescence spectroscopy has been previously used on humans and other mammals to measure endogenous autofluorescent species of skin and other tissues (Leffell et al 1988;Kollias et al 1998;Brancaleon et al 1999;Gillies et al ., 2000;Doukas et al ., 2001;Na et al ., 2001;Sandby-Moller et al ., 2003). Fluorescent species that are common in many biological tissues include the aromatic amino acids (tryptophan, tyrosine and phenylalanine), cross-linked collagen and elastin, lipofuscin, NADH, and FAD.…”

Section: Autofluorescent Species Can Be Measured In Living C Elegansmentioning

confidence: 99%

In vivospectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction inCaenorhabditis elegans

et al. 2005

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SummaryAutofluorescent lipofuscin and advanced glycation end-products (age pigments) accumulate with age across phyla, yet little is understood about their formation under physiological conditions and their specific contributions to the aging process. We used in vivo spectrofluorimetry to quantitate autofluorescence in wild-type Caenorhabditis elegans and longevity mutants disrupted for distinct aspects of the aging process. In wild-type animals, age pigments increase into adulthood, accumulating slowly during the reproductive phase and more rapidly during the post-reproductive period. As in humans, insulin signaling influences age pigment accumulation -mutations that lower efficacy of insulin signaling and extend lifespan [ daf-2 ( e1370 ) insulin receptor and age-1 ( hx546 ) PI3-kinase] dramatically lower age pigment accumulation; conversely, elimination of the insulin-inhibited DAF-16/ FOXO transcription factor causes a huge increase in age pigment accumulation, supporting that the short-lived daf-16 null mutant is truly progeric. By contrast, mutations that increase mitochondrial reactive oxygen species production do not affect age pigment accumulation, challenging assumptions about the role of oxidative stress in generating these species in vivo . Dietary restriction reduces age pigment levels significantly and is associated with a unique spectral shift that might serve as a rapidly scored reporter of the dietary restricted state. Unexpectedly, genetically identical siblings that age poorly (as judged by decrepit locomotory capacity) have dramatically higher levels of age pigments than their same-aged siblings that appear to have aged more gracefully and move youthfully. Thus, high age pigment levels indicate a physiologically aged state rather than simply marking chronological time, and age pigments are valid reporters of nematode healthspan.

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Section: Autofluorescent Species Can Be Measured In Living C Elegansmentioning

confidence: 99%

In vivospectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction inCaenorhabditis elegans

et al. 2005

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“…Recently, in vivo noninvasive methods that can objectively measure parameters related to skin biology and that complement the more subjective clinical evaluation have been developed [10] . In the case of skin aging, in vivo fluorescence spectroscopy provides an important marker that has been shown to relate epidermal thickness and epidermal cell proliferation rate in mice [11] and in humans [12][13][14] . Up to now, fluorescence spectroscopy has not been used to measure epidermal proliferation induced by ROL.…”

Section: Introductionmentioning

confidence: 99%

Antiaging Action of Retinol: From Molecular to Clinical

Bellemère

Stamatas

Bruère

et al. 2009

Skin Pharmacol Physiol

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The antiaging efficacy of retinol (ROL) has been explored mainly clinically in photoprotected skin sites and for high doses of ROL (0.4–1.6%). The objective of the study was to demonstrate the antiaging action of a low and tolerable dose of ROL (0.1%) ex vivo by measuring the expression of cellular retinoic-acid-binding protein II (CRABP2) and heparin-binding epidermal growth factor (HBEGF) by a histological evaluation of the epidermis and in vivo by assessing major aging signs and performing three-dimensional profilometry and digital imaging during a 9-month double-blind placebo-controlled study involving 48 volunteers. Finally, epidermal cell proliferation was evaluated using tryptophan fluorescence spectroscopy. Our results demonstrate that 0.1% ROL induced CRABP2 and HBEGF gene expression and increased keratinocyte proliferation and epidermal thickness. In human volunteers, topical application of a ROL-containing product improved all major aging signs assessed in our study (wrinkles under the eyes, fine lines and tone evenness). Moreover, tryptophan fluorescence increased in the active-agent-treated group and not in the placebo-treated group, indicating that cell proliferation was accelerated in vivo. These data demonstrate that a product containing a low dose (0.1%) of ROL promotes keratinocyte proliferation ex vivo and in vivo, induces epidermal thickening ex vivo and alleviates skin aging signs, without any significant adverse reaction.

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“…In youth, the various physiological stimulations of melanogenesis increase evenly the eumelanin pigmentation or induce phaeomelanin-enriched freckles without any prominent change in the melanocyte density. By contrast, intense and chronic ultraviolet light exposures result in a mottled appearance typical of photo-ageing and PUVA-induced changes [1][2][3][4][5][6]. Indeed, the number, distribution and synthetic activity of melanocytes become uneven in such conditions, as well as the capacity to transfer melanosomes to keratino- Hermanns/Petit/Piérard-Franchimont/ Paquet/Piérard cytes.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Topical Hypopigmenting Agents on Solar Lentigines of Asian Women

et al. 2002

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Background: So-called darkened age spots encompass distinct pathological processes. The efficacy of topical depigmenting agents is difficult to objectivate. Objective: To assess the hypopigmenting effect of three cosmetic formulations using objective biometrological methods. Methods: 50 women of South-East Asian ancestry were enrolled in this pilot study. They had solar lentigines according to dermoscopic criteria. The lesions were treated by topical hypopigmenting formulations. Products were applied twice daily for 2 or 3 months. Assessments at 1-month intervals were made using narrow-band reflectance spectrophotometry, image analysis of video-recorded ultraviolet light reflection and photodensitometry- and image-analysis-assisted corneomelametry. Results: A 20% azelaic acid formulation and another one containing 5% ascorbyl glucosamine, 1% kojic acid and α-hydroxyacid esters appeared inefficacious on solar lentigines. A stabilized soy extract showed a better although modest lightening effect when assessed by corneomelametry. The subclinical or faint mottled skin revealed by ultraviolet light examination better responded (p < 0.05) to treatments. Conclusion: Focal epidermal hyperpigmentation is better controlled by topical whitening agents when the increase in melanin content reflects a modest functional hyperactivity of melanocytes.

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In vivo fluorescence of human skin. A potential marker of photoaging

Cited by 52 publications

References 0 publications

In vivospectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction inCaenorhabditis elegans

In vivospectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction inCaenorhabditis elegans

Antiaging Action of Retinol: From Molecular to Clinical

Assessment of Topical Hypopigmenting Agents on Solar Lentigines of Asian Women

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