In Vivo DNA Affinity Purification and Histone Deacetylase Inhibitor Treatment Proves the Role of Histone Acetylation in the Expression Regulation of High-Molecular-Weight Glutenin Genes

Éva, Csaba; Szőke-Pázsi, Kitti; Makai, Szabolcs; Gell, Gyöngyvér; Fábián, Attila; Poczkodi, Edina; Tóth, Gábor; Sági, László; Tamás, László; Juhász, Angéla

doi:10.1007/s11105-018-1117-8

Cited by 2 publications

(9 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The inhibiting effect of Myb TFs in HMW GS expression in vegetative tissues has not been demonstrated yet, though it is common for other prolamin genes (Suzuki et al 1998;Chen et al 2005). Our transcriptional co-expression network analysis (Makai et al 2015) revealed-among others-an early gene circuit of TFs expressing at the very beginning of endosperm development when HMW GS genes are not active yet; this circuit therefore may contain inhibitory TFs (Makai et al 2015;Éva et al 2018).…”

Section: Introductionmentioning

confidence: 79%

“…identity to Tae064063 in our earlier publications, RefSeq ID: TraesC-S5D02G447500) and TabZIP115 (76.8% aa. identity to Tae057404 in our earlier publications, RefSeq ID: TraesC-SU02G019200) which express at early phase (0-5 DPA) of endosperm development (Makai et al 2015;Éva et al 2018). HMW GS genes do not express at this developmental stage and therefore this programme may contain their suppressor factors (Makai et al 2015;Éva et al 2018).…”

Section: Confirmation Of the Promoter-binding Affinity Using Emsamentioning

confidence: 87%

“…identity to Tae057404 in our earlier publications, RefSeq ID: TraesC-SU02G019200) which express at early phase (0-5 DPA) of endosperm development (Makai et al 2015;Éva et al 2018). HMW GS genes do not express at this developmental stage and therefore this programme may contain their suppressor factors (Makai et al 2015;Éva et al 2018). Indeed, both TabZIP34 and TabZIP115 show much higher expression in grains at 2 DPA than at later stages (14-35 DPA), but TabZIP94 is also highly expressed later, according to ExpVIP wheat expression browser (Fig.…”

Section: Confirmation Of the Promoter-binding Affinity Using Emsamentioning

confidence: 92%

“…The synthesis of seed-storage proteins like HMW GS is also influenced by environmental factors such as the temperature, as well as water, sulphur and nitrogen availability (Luo et al 2000;Wardlaw et al 2002;Xu et al 2020). A focused transcriptional co-expression network constructed for HMW GS genes and regulating factors (Makai et al 2015;Éva et al 2018) revealed that the Glu-1 paralogues (x-and y-type genes) belong to different regulatory circuits. The x-type genes were suggested to rely on Myb TFs, while the y-type genes are driven by NAC TFs during the late stage of grain filling (Makai et al 2015), which may account for their differential regulation.…”

Section: Introductionmentioning

confidence: 99%

“…As it was mentioned, HMW GS gene expression is blocked in vegetative tissues and early (0-5 days postanthesis, DPA) endosperm. The lack of histone acetylation has been proven as being partially responsible (Guo et al 2015;Éva et al 2018). The inhibiting effect of Myb TFs in HMW GS expression in vegetative tissues has not been demonstrated yet, though it is common for other prolamin genes (Suzuki et al 1998;Chen et al 2005).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

bZIP transcription factors repress the expression of wheat (Triticum aestivum L.) high molecular weight glutenin subunit genes in vegetative tissues

et al. 2023

Self Cite

View full text Add to dashboard Cite

High molecular weight glutenin subunits (HMW GS) represent an important fraction of endosperm-specific seed-storage proteins that provide elasticity to bread dough. Previously, the second cis-regulatory module (CRM2) was found to be one of the most conserved part of HMW GS promoters, which indicated its pre-eminent role in their gene regulation. Here, we observed that deletion of CRM2 from the promoters of the Bx7 and By8 HMW GS genes increased the leakage of their transient expression in wheat leaf tissue. The effect of a VP1, an Myb and an antisense bZIP transcription factor (TF)-binding site, potentially involved in endosperm-specific regulation within CRM2, was then studied. The activity of a Bx7 gene promoter containing a mutant CRM2 with altered VP1 and Myb TF-binding sites, but an intact bZIP TF-binding site, was similarly low to that of the wild type in leaves. Transactivation analysis and EMSA indicated the binding of TFs TabZIP34 and TabZIP115 to the Skn-1 motif GTCAT in CRM2 and the repression of Bx7 and By8 HMW GS gene promoter activity in leaves. TabZIP34 and TabZIP115 may be involved in the downregulation of HMW GS gene expression in vegetative tissues and early-stage endosperm as well its modulation during seed maturation.

show abstract

Section: Introductionmentioning

confidence: 79%

Section: Confirmation Of the Promoter-binding Affinity Using Emsamentioning

confidence: 87%

Section: Confirmation Of the Promoter-binding Affinity Using Emsamentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

bZIP transcription factors repress the expression of wheat (Triticum aestivum L.) high molecular weight glutenin subunit genes in vegetative tissues

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

Identifying cis-Acting Elements Associated with the High Activity and Endosperm Specificity of the Promoters of Genes Encoding Low-Molecular-Weight Glutenin Subunits in Common Wheat (Triticum aestivum)

Qu,

Wang,

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Low-molecular-weight glutenin subunits (LMW-GSs) associated with bread-baking quality and flour nutrient quality accumulate in endosperms of common wheat and related species. However, the mechanism underlying the expression regulation of genes encoding LMW-GSs has not been fully elucidated. In this study, we identified LMW-D2 and LMW-D7, which are highly and weakly expressed, respectively, via the analysis of RNA-sequencing data of Chinese Spring wheat and wheat transgenic lines transformed with 5′ deletion promoter fragments and GUS fusion constructs. The 605-bp fragment upstream of the LMW-D2 start codon could drive high levels of GUS expression in the endosperm. The truncated endosperm box located at the −300 site resulted in the loss of LMW-D2 promoter activity, and a single-nucleotide polymorphism on the GCN4 motif was closely related to the expression of LMW-GSs. TCT and TGACG motifs, as well as the others located on the 5′ distal end, might also be involved in the transcription regulation of LMW-GSs. In transgenic lines, fusion proteins of LMW-GS and GUS were deposited into protein bodies. Our findings provide new insights into the mechanism underlying the transcription regulation of LMW-GSs and will contribute to the development of wheat endosperm as a bioreactor for the production of nutraceuticals, antibodies, vaccines, and medicinal proteins.

show abstract

In Vivo DNA Affinity Purification and Histone Deacetylase Inhibitor Treatment Proves the Role of Histone Acetylation in the Expression Regulation of High-Molecular-Weight Glutenin Genes

Cited by 2 publications

References 56 publications

bZIP transcription factors repress the expression of wheat (Triticum aestivum L.) high molecular weight glutenin subunit genes in vegetative tissues

bZIP transcription factors repress the expression of wheat (Triticum aestivum L.) high molecular weight glutenin subunit genes in vegetative tissues

Identifying cis-Acting Elements Associated with the High Activity and Endosperm Specificity of the Promoters of Genes Encoding Low-Molecular-Weight Glutenin Subunits in Common Wheat (Triticum aestivum)

Contact Info

Product

Resources

About