In vivo antioxidative effect of isoquercitrin on cadmium-induced oxidative damage to mouse liver and kidney

Abstract

Cadmium (Cd) is an environmental pollutant and has been found to pose a potential threat to human health. Isoquercitrin (IQ) is one of the most important flavonoids and has been demonstrated to exhibit potent antioxidant effects on plants and yeast cells. However, only few studies have investigated the antioxidative activities of reactive oxygen species (ROS) and the nitrite scavenging activities of IQ against Cd-induced oxidation in mouse. The present work was to investigate the ROS and nitrite-scavenging activities of IQ in vitro as well as its preventive effects against lipid peroxidation and protein oxidative damage in liver and kidney of mouse induced by Cd(²+) using spectrophotometry. Our results showed that IQ possesses scavenging abilities for superoxide anion, hydroxyl radical and nitrite. Such scavenging capacities increase with the concentration of IQ. Moreover, cadmium chloride (CdCl₂ (2.5 mg/kg body weight, i.p. CdCl₂) significantly inhibited the activities of superoxide dismutase and catalase and raised the levels of malondialdehyde, nitric oxide, protein carbonyl, and the coefficients of DNA-protein crosslinks in livers and/or kidneys of mice. IQ attenuated the Cd(²+)-induced biochemical alterations in the livers and/or kidneys of mice, indicating that the formation of ROS and nitrite is possibly reduced. Our work demonstrates that IQ possesses ROS and nitrite-scavenging capacities and plays a significant role in combating Cd(²+)-induced toxicity in animals.

Protective effects of quercetin glycosides, rutin, and isoquercetrin against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in rat pheochromocytoma (PC-12) cells

“…27 Several studies have reported that pretreatment with isoquercitrin increased the cell tolerance to oxidative stress. [28][29][30] Along with this, flavonoids with antioxidant activity such as quercetin have a high binding affinity towards catalase enzyme in the cells. 29 The binding of quercetin with catalase induced some conformational changes on this enzyme, which results in enhanced ability to quench free radicals as well as increase the antioxidant status in cells.…”

Amelioration of particulate matter-induced oxidative damage by vitamin c and quercetin in human bronchial epithelial cells

“…Quercetin absolutely prevented mercury chloride (HgCl 2 )-induced kidney injury of SpragueeDawley rats (Shin et al, 2015). Similarly, isoquercitrin (IQ) possessed ROS and nitrite-scavenging capacities and played a significant role in combating Cd(2þ)-induced toxicity in animals (Li et al, 2011).…”

Review of anticancer mechanisms of isoquercitin

“…This action is mediated by numerous mechanisms including the follow ing: Protecting the cell membrane by inhibiting LDH efflux [34] ; maintaining erythrocyte deformability, which plays an essential role in circulatory system by preventing vascular diseases [35] ; accumulating in the arterial wall and preventing hyperlipidemia [36] ; inhibiting fatty acid formation resulting from lipid peroxidation [37] ; protecting neural cells against oxidative stress induced by H2O2 by Orfali GC et al . Isoquercitrin in cancer decreasing lipid peroxidation and increasing cellular chole sterol concentrations [38] ; reducing glutathione depletion and decreasing catalase and glutathione peroxidase 1 activities [39] ; ROS and nitritescavenging activities [40] ; and by regulating the oxidative complex NADPH and NO production [41] , lipidlowering activities in hepatocytes [42] , induction of apolipoprotein AI gene expression [43] , and free radical scavenging [4464] .…”