In vivo and in vitro anti-inflammatory and pro-osteogenic effects of citrus cystatin CsinCPI-2

Leguizamón, Natalia Da Ponte; Rodrigues, Érico; Campos, Marcelo Lattarulo; Nogueira, Andressa Vilas Boas; Viola, Kennia Scapin; Schneider, Vanessa Karine; Neo-Justino, Daniela Morilha; Tanomaru‐Filho, Mário; Zambuzzi, Willian Fernando; Henrique-Silva, Flávio; Soares-Costa, Andréa; Faria, Gisele; Cirelli, Joni Augusto

doi:10.1016/j.cyto.2019.154760

Cited by 21 publications

(23 citation statements)

References 53 publications

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“…2011, Komori 2011, Leguizamón et al . 2019) in comparison with the control group. BMP‐2 has a decisive role during the bone formation, regeneration and its repair processes (Ruppert et al .…”

Section: Discussionmentioning

confidence: 89%

“…The exposure of hDPCs to CaneCPI-1 promoted the upregulation of genes related to osteogenesis BMP-2, RUNX2, ALP, BSP and OC (Bakopoulou et al 2011, Komori 2011, Leguizam on et al 2019 in comparison with the control group. BMP-2 has a decisive role during the bone formation, regeneration and its repair processes (Ruppert et al 1996, Wu et al 2007, acting directly on bone progenitors and promoting bone formation (Santo et al 2013).…”

Section: Discussionmentioning

confidence: 89%

“…The concentrations (3.73, 7.47, 16.60 and. 33.20 µm) were chosen according to a previous study (Leguizam on et al 2019), in which a citrus cystatin (CsinCPI-2) showed cytocompatibility and bioactivity, mainly at the 3.73 µm concentration. hDPCs treated with CaneCPI-1 displayed no apoptosis and showed a higher percentage of viability compared to the control group.…”

Section: Discussionmentioning

confidence: 99%

“…Considering the potential of cystatins to induce osteogenic phenotype (Danjo et al 2007, Leguizam on et al 2019, the aim of this study was to evaluate the cytocompatibility and osteogenic potential of CaneCPI-1 in human dental pulp cells (hDPCs). The null hypothesis is that CaneCPI-1 is not biocompatible and has no potential to induce the differentiation of undifferentiated human dental pulp cell in osteogenic phenotype.…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that a new phytocystatin derived from Citrus sinensis, named C sin CPI‐2, promoted inhibitory activity of cathepsins K and B, resulting in potential anti‐inflammatory and pro‐osteogenic effects in human dental pulp cells by an increase in alkaline phosphatase (ALP) activity, deposition nodules and gene expression of osteogenic markers (Leguizamón et al . 2019). The authors concluded that the anti‐inflammatory and pro‐osteogenic CsinCPI‐2 effects may lead to new therapies for control of periodontal disease and apical periodontitis.…”

Section: Introductionmentioning

confidence: 99%

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Sugarcane cystatin CaneCPI‐1 promotes osteogenic differentiation in human dental pulp cells: a new insight into cysteine proteases inhibitors

Rodrigues¹,

Viola²,

Gomes-Cornélio³

et al. 2020

Int Endodontic J

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Sugarcane cystatin CaneCPI‐1 promotes osteogenic differentiation in human dental pulp cells: a new insight into cysteine proteases inhibitors

Rodrigues¹,

Viola²,

Gomes-Cornélio³

et al. 2020

Int Endodontic J

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Remotely Temporal Scheduled Macrophage Phenotypic Transition Enables Optimized Immunomodulatory Bone Regeneration

Huang

et al. 2022

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Precise timing of macrophage polarization plays a pivotal role in immunomodulation of tissue regeneration, yet most studies mainly focus on M2 macrophages for their anti‐inflammatory and regenerative effects while the essential proinflammatory role of the M1 phenotype on the early inflammation stage is largely underestimated. Herein, a superparamagnetic hydrogel capable of timely controlling macrophage polarization is constructed by grafting superparamagnetic nanoparticles on collagen nanofibers. The magnetic responsive hydrogel network enables efficient polarization of encapsulated macrophage to the M2 phenotype through the podosome/Rho/ROCK mechanical pathway in response to static magnetic field (MF) as needed. Taking advantage of remote accessibility of magnetic field together with the superparamagnetic hydrogels, a temporal engineered M1 to M2 transition course preserving the essential role of M1 at the early stage of tissue healing, as well as enhancing the prohealing effect of M2 at the middle/late stages is established via delayed MF switch. Such precise timing of macrophage polarization matching the regenerative process of injured tissue eventually leads to optimized immunomodulatory bone healing in vivo. Overall, this study offers a remotely time‐scheduled approach for macrophage polarization, which enables precise manipulation of inflammation progression during tissue healing.

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Effects of Cyanobacterial Metabolites on Other Bacterial Phyla and in the Morphogenesis, Viability, and Biochemistry of Eukaryotes

Villa

Feijoo

Sánchez

et al. 2021

Developmental Biology in Prokaryotes and Lower Eukaryotes

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In vivo and in vitro anti-inflammatory and pro-osteogenic effects of citrus cystatin CsinCPI-2

Cited by 21 publications

References 53 publications

Sugarcane cystatin CaneCPI‐1 promotes osteogenic differentiation in human dental pulp cells: a new insight into cysteine proteases inhibitors

Sugarcane cystatin CaneCPI‐1 promotes osteogenic differentiation in human dental pulp cells: a new insight into cysteine proteases inhibitors

Remotely Temporal Scheduled Macrophage Phenotypic Transition Enables Optimized Immunomodulatory Bone Regeneration

Effects of Cyanobacterial Metabolites on Other Bacterial Phyla and in the Morphogenesis, Viability, and Biochemistry of Eukaryotes

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