In Vivo Analysis of Drosophila bicoid mRNA Localization Reveals a Novel Microtubule-Dependent Axis Specification Pathway

Cha, Byeong-Jik; Koppetsch, Birgit S.; Theurkauf, William E.

doi:10.1016/s0092-8674(01)00419-6

Cited by 186 publications

(188 citation statements)

References 39 publications

Supporting

Mentioning

168

Contrasting

Unclassified

Order By: Relevance

“…Oocytes contain many different populations of microtubules that reorganize and change throughout different developmental stages. The microtubule network is initially polarized during early stages, with the majority of plus-ends at the posterior pole and minus-ends at the anterior pole (55,56), but later becomes less organized, with minus-ends associating with the cortex (31,(57)(58)(59)(60)(61). Microtubules are also concentrated at the posterior pole during early stages, and these microtubules reorganize and relocate to the anterior pole during stages 8-10A, forming a gradient of microtubules from anterior to posterior.…”

Section: Discussionmentioning

confidence: 99%

Microtubule–microtubule sliding by kinesin-1 is essential for normal cytoplasmic streaming in Drosophila oocytes

Lü

Winding

Lakonishok

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

122

View full text Add to dashboard Cite

Cytoplasmic streaming in Drosophila oocytes is a microtubulebased bulk cytoplasmic movement. Streaming efficiently circulates and localizes mRNAs and proteins deposited by the nurse cells across the oocyte. This movement is driven by kinesin-1, a major microtubule motor. Recently, we have shown that kinesin-1 heavy chain (KHC) can transport one microtubule on another microtubule, thus driving microtubule-microtubule sliding in multiple cell types. To study the role of microtubule sliding in oocyte cytoplasmic streaming, we used a Khc mutant that is deficient in microtubule sliding but able to transport a majority of cargoes. We demonstrated that streaming is reduced by genomic replacement of wild-type Khc with this sliding-deficient mutant. Streaming can be fully rescued by wild-type KHC and partially rescued by a chimeric motor that cannot move organelles but is active in microtubule sliding. Consistent with these data, we identified two populations of microtubules in fast-streaming oocytes: a network of stable microtubules anchored to the actin cortex and free cytoplasmic microtubules that moved in the ooplasm. We further demonstrated that the reduced streaming in sliding-deficient oocytes resulted in posterior determination defects. Together, we propose that kinesin-1 slides free cytoplasmic microtubules against cortically immobilized microtubules, generating forces that contribute to cytoplasmic streaming and are essential for the refinement of posterior determinants.kinesin-1 | microtubules | cytoplasmic streaming | Drosophila | axis determination

show abstract

Section: Discussionmentioning

confidence: 99%

Microtubule–microtubule sliding by kinesin-1 is essential for normal cytoplasmic streaming in Drosophila oocytes

Lü

Winding

Lakonishok

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

122

View full text Add to dashboard Cite

show abstract

“…2; see below). The posterior MTOC is disassembled in stages 6 -7, and several studies suggest that thereafter MTs emanate from the entire oocyte cortex (Cha et al, 2001(Cha et al, , 2002. These cortical MTs initially point toward the center of the oocyte, but in stage 8 and 9 oocytes, cortical MTs are cleared specifically from the posterior pole, allowing for the refocusing of MT plus-ends at or near the posterior ( Fig.…”

Section: After Stage 6 the Oocyte Mts Reorganize To Form The Mt Arramentioning

confidence: 99%

“…4G). More recent studies suggest that MTs emanate from the lateral cortices as well (Cha et al, 2001(Cha et al, , 2002. Staining with antibodies for MTOC components has yielded conflicting results.…”

Section: The Mechanism Governing Mt Organization In the Midoogenesis mentioning

confidence: 99%

Microtubule polarity and axis formation in theDrosophila oocyte

2006

View full text Add to dashboard Cite

The body axes of the fruit fly are established in mid-oogenesis by the localization of three mRNA determinants, bicoid, oskar, and gurken, within the oocyte. General mechanisms of RNA localization and cell polarization, applicable to many cell types, have emerged from investigation of these determinants in Drosophila oogenesis. Localization of these RNAs is dependent on the germline microtubules, which reorganize to form a polarized array at mid-oogenesis in response to a signaling relay between the oocyte and the surrounding somatic follicle cells. Here we describe what is known about this microtubule reorganization and the signaling relay that triggers it. Recent studies have identified a number of ubiquitous RNA binding proteins essential for this process. So far, no targets for any of these proteins have been identified, and future work will be needed to illuminate how they function to reorganize microtubes and whether similar mechanisms also exist in other cell types.

show abstract

“…Several different model systems have been used for dissecting RNA transport. In Drosophila oocytes, microtubules show gross polarity with the plus-end at the posterior and the minus-end at the anterior of the oocytes (7,8). It has been shown that localization of oskar mRNA to the posterior pole is mediated by kinesin-1 (9, 10), whereas anteriordorsal localization of gurken mRNA and anterior localization of bicoid mRNA is mediated by dynein (8,11).…”

mentioning

confidence: 99%

Transport of Drosophila fragile X mental retardation protein-containing ribonucleoprotein granules by kinesin-1 and cytoplasmic dynein

Ling

Fahrner

Greenough

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

158

142

View full text Add to dashboard Cite

Transport and translation of mRNA are tightly coupled to ensure strict temporal and spatial expression of nascent proteins. Fragile X mental retardation protein (FMRP) has been shown to be involved in translational regulation and is found in ribonucleoprotein (RNP) granules that travel along dendrites of neurons. In this study, GFP-tagged Drosophila homologue of FMRP (dFMR) was used to visualize RNP granule movement in Drosophila S2 cells. GFP-dFMR form granules that contain both endogenous dFMR and mRNA. Live fluorescence microscopy revealed that dFMR-containing RNP granules move bidirectionally in thin processes formed by S2 cells in the presence of cytochalasin D. Knocking down the heavy chains of either kinesin-1 (kinesin heavy chain) or cytoplasmic dynein (dynein heavy chain) by RNA interference blocks the movement of the dFMR granules. In contrast, knockdown of kinesin light chain (KLC), which is typically necessary for movement of membrane organelles by kinesin-1, had no effect on the dFMR granule translocation. In immunoprecipitation assays, dFMR associates with both kinesin heavy chain and dynein heavy chain, but not KLC. Based on these findings, we conclude that dFMR-containing RNP granules are moved by both kinesin-1 and cytoplasmic dynein and that KLC is not essential and is likely missing from RNP-transporting kinesin-1.microtubules ͉ molecular motors ͉ cytoskeleton ͉ RNA transport

show abstract

In Vivo Analysis of Drosophila bicoid mRNA Localization Reveals a Novel Microtubule-Dependent Axis Specification Pathway

Cited by 186 publications

References 39 publications

Microtubule–microtubule sliding by kinesin-1 is essential for normal cytoplasmic streaming in Drosophila oocytes

Microtubule–microtubule sliding by kinesin-1 is essential for normal cytoplasmic streaming in Drosophila oocytes

Microtubule polarity and axis formation in theDrosophila oocyte

Transport of Drosophila fragile X mental retardation protein-containing ribonucleoprotein granules by kinesin-1 and cytoplasmic dynein

Contact Info

Product

Resources

About