2011
DOI: 10.1007/s10616-011-9337-x View full text |Buy / Rent full text
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Abstract: The aim of the study was to develop a new strategy for the differentiation of hematopoietic stem cell (HSC) derived from UCB into hepatocyte like cells and also to estimate the effects of combination of fibroblast growth factor 4 (FGF 4) and hepatocyte growth factor (HGF) on hematopoietic stem cell differentiation. HSCs were isolated and purified by magnetic activated cell sorting. HSCs were induced to hepatocyte like cells under a 2-step protocol with combination of growth factors. Reverse transcription polym… Show more

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“…However, CD34 + CD38 − cells indeed conserve more primitive HSCs population, which contain more efficiency to reconstitute all blood cell types in vivo . The CD34 + CD38 − cells also contain the plasticity of differentiation into many cell types in vitro such as adipocytes [6], brain cells (neurons and astrocytes) [7], cardiomyocytes [8], liver cells [9], myoblasts [10], myoendothelial [11], osteochondrocytes [12], and pancreatic cells [13]. The CD34 + populations from bone marrow and cord blood are heterogeneous and contain both CD34 + CD38 − and CD34 + CD38 + fractions.…”
Section: Introductionmentioning
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“…However, CD34 + CD38 − cells indeed conserve more primitive HSCs population, which contain more efficiency to reconstitute all blood cell types in vivo . The CD34 + CD38 − cells also contain the plasticity of differentiation into many cell types in vitro such as adipocytes [6], brain cells (neurons and astrocytes) [7], cardiomyocytes [8], liver cells [9], myoblasts [10], myoendothelial [11], osteochondrocytes [12], and pancreatic cells [13]. The CD34 + populations from bone marrow and cord blood are heterogeneous and contain both CD34 + CD38 − and CD34 + CD38 + fractions.…”
Section: Introductionmentioning
“…After 24 h of incubation, the fraction of the surviving cells was evaluated using MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] assay as suggested by Mossman . The reaction involves the conversion of tetrazolium salt [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide], a pale yellow substrate to formazan, which is a dark blue product, by the active mitochondria in living cells only . The cells were incubated with MTT for 4–5 h for the reaction between the MTT and mitochondrial dehydrogenase of the live cells.…”
Section: Methodsmentioning
“…Hepatocyte-like cells featured a polygonal shape, expressed AFP, albumin, G6P and CK-18. One of the novel aspects of this study was the shorter induction timeframe to obtain HLCs: only 14 days, as compared to 21 days timeframes reported in other works [123]. Clearly, more stringent assays will be needed in order to assess the adherence of these cells to the mature hepatocyte phenotype.…”
Section: Umbilical Cord Blood-derived Mscsmentioning