In vitro tracking of phospholipase A2 from snake venom conjugated with magic-sized quantum dots

Fluorescent Markers: Proteins and Nanocrystals

“…[43,44] Dias et al labeled a phospholipase A2 isolated from Bothrops alternatus snake venom with CdSe/CdS MSQDs to track it in myoblast culture, making it possible to identify the bioconjugate on the surface of the plasma membrane and in the nuclear region. [39] Figure 7 corroborates these data since it is possible to observe fluorescence markings only in myoblasts treated with the bioconjugate MSQDs-BaltPla2.…”

“…Bioimaging assays are biological applications QDs since they can be bioconjugated with proteins, antibodies, and DNA. [39,48,49]. In general, these tests depend on the biocompatibility of QDs, which is obtained by functionalizing the surface of these nanoparticles.…”

“…In general, these tests depend on the biocompatibility of QDs, which is obtained by functionalizing the surface of these nanoparticles. [39,[50][51][52] The bioconjugation allows the study and tracking of biomolecules in biological systems such as cell cultures and laboratory animals. [53,54] The versatility of QDs associated with maltosebinding protein for intracellular delivery of the drug beta-cyclodextrin.…”

“…[56] The tracking and study of biomolecules labeled with QDs in vitro and in vivo is a reality in several areas, allowing us to analyze the location and distribution of bioconjugate in biological systems. Silva et al demonstrated that the CdSe/ CdS MSQDs could be bioconjugated with several biomolecules aiming at the most diverse luminescent probes [38][39][40][41][42] in biosensors. [43,44] Dias et al labeled a phospholipase A2 isolated from Bothrops alternatus snake venom with CdSe/CdS MSQDs to track it in myoblast culture, making it possible to identify the bioconjugate on the surface of the plasma membrane and in the nuclear region.…”

“…[33] Fluorescent probes based on organic dyes have been shown to identify biomolecules. [34,35] Silva et al demonstrated that the biocompatibility of CdSe/CdS MSQRd could be tuned in the synthesis, [36] present high luminescence stability in biological systems [37], can be bioconjugated with several biomolecules aiming at the most diverse luminescent probes [38][39][40][41][42] and in biosensors. [43,44] This chapter shows recent results that the group has been working with fluorescent reporter proteins and the applicability of nanocrystals as fluorescent markers.…”