In vitro toxicity testing in hemocytes of the marine mussel Mytilus galloprovincialis (L.) to uncover mechanisms of action of the water accommodated fraction (WAF) of a naphthenic North Sea crude oil without and with dispersant

Katsumiti, Alberto; Nicolussi, Greta; Bilbao, Dennis; Prieto, Ailette; Etxebarria, Néstor; Cajaraville, Miren P.

doi:10.1016/j.scitotenv.2019.03.187

Cited by 30 publications

(7 citation statements)

References 71 publications

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“…Results manifested that ROS production increased in the respiratory tree of sea cucumber exposed to WAF, and the effect was much more obvious when exposed to CEWAF. The increase in ROS production observed in the present study is consistent with recent reports supporting that oil-derived hydrocarbons could elevate ROS production and the addition of chemical dispersant could enhance the elevation of ROS production in marine benthos, such as marine mussel (Mytilus galloprovincialis) [7] and eastern oyster (Crassostrea virginica) [24]. It is well known that the antioxidant enzymatic system is the primary defense line to scavenge the excessive ROS and maintain the cellular redox homeostasis within certain limits [46,47].…”

Section: Discussionsupporting

confidence: 93%

Antioxidant Response and Oxidative Stress in the Respiratory Tree of Sea Cucumber (Apostichopus japonicus) Following Exposure to Crude Oil and Chemical Dispersant

Liao

et al. 2020

JMSE

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Sea cucumber (Apostichopus japonicus) is mainly cultured in the coastal zone, where it is easily threatened by accidental oil spills. Chemical dispersant is one of the efficient oil spill responses for mitigating the overall environmental damage of oil spills. However, the impact of crude oil and chemical dispersants on sea cucumber is less well known. Hence, the present study focused on exploring the antioxidant response and oxidative stress in the respiratory tree of sea cucumber following exposure to GM-2 chemical dispersant (DISP), water-accommodated fractions (WAF), and chemically enhanced WAF (CEWAF) of Oman crude oil for 24 h. Results manifested that WAF exposure caused a significant increase in the reactive oxygen species (ROS) level (5.29 ± 0.30 AU·mgprot−1), and the effect was much more obvious in CEWAF treatment (5.73 ± 0.16 AU·mgprot−1). Total antioxidant capacity (T-AOC), as an important biomarker of the antioxidant defense capacity, showed an increasing trend following WAF exposure (0.95 ± 0.12 U·mgprot−1) while a significant reduction in T-AOC was observed following CEWAF exposure (0.23 ± 0.13 U·mgprot−1). Moreover, we also evaluated the oxidative damage of the macromolecules (DNA, protein, and lipid), and our results revealed that the presence of chemical dispersant enhanced oxidative damage caused by crude oil to sea cucumber.

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Section: Discussionsupporting

confidence: 93%

Antioxidant Response and Oxidative Stress in the Respiratory Tree of Sea Cucumber (Apostichopus japonicus) Following Exposure to Crude Oil and Chemical Dispersant

Liao

et al. 2020

JMSE

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“…During the response to the disaster, more than seven million liters of synthetic dispersants (Corexit EC9500A and EC9527A) were applied to surface oil slicks, and the discharging wellhead at 1500 m depth 1,2 . This unprecedent application of dispersants enhanced oil droplet formation, aqueous phase solubilization and aimed to increase biodegradation at depth, but may have had negative effects on the microbial communities [3][4][5] and marine organisms [6][7][8][9] . Though dispersant application is a common response to oil spills, their effects on marine microbial populations is unclear, and the impacts on hydrocarbon biodegradation are debated 10,11 .…”

Section: Introductionmentioning

confidence: 99%

ColwelliaandMarinobactermetapangenomes reveal species-specific responses to oil and dispersant exposure in deepsea microbial communities

Peña-Montenegro

Kleindienst

Allen

et al. 2020

Preprint

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Over 7 million liters of Corexit EC9500A and EC9527A were applied to the Gulf of Mexico in response to the Deepwater Horizon oil spill. The impacts of dispersants remain under debate and negative, positive, and inconclusive impacts have been reported. Here, metatrancriptomics was applied in the context of metapangenomes to microcosms that simulated environmental conditions comparable to the hydrocarbon-rich 1,100 m deep plume. Within this microcosm study, negative effects of dispersants on microbial hydrocarbon degradation were previously reported based on activity measurements and geochemical data. Transcriptional enrichment of Colwellia, a potential dispersant degrader, followed variable time-dependent trajectories due to interactions between oil, dispersants, and nutrients. The Colwellia metapangenome captured a mixture of environmental responses linked to the Colwellia psychrerythraea 34H genome and to the genomes of other members of the Colwellia genus. The activation of genes involved in lipid degradation, nitrogen metabolism, and membrane composition under oil or nutrient availability, suggested an opportunistic growth strategy for Colwellia. In contrast, transcripts of Marinobacter, a natural hydrocarbon degrader, increased only in oil treatments. Marinobacter transcripts largely recruited to the accessory metapangenome of Marinobacter sp. C18, the closest genomic reference. A complex response involving carbon and lipid metabolism, chemotaxis and a type IV secretion system suggested active energy-dependent processes in Marinobacter. These findings highlight chemistry-dependent responses in the metabolism of key hydrocarbon-degrading bacteria and underscore that dispersant-driven selection could temper the ability of the community to respond to hydrocarbon injection.

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“…The WAF produced from the NNS oil presented a low PAH concentration (ƩPAHs = 300.94 μg/L; Supplementary Table S5) as expected for a low-energy WAF (Singer et al, 2000;Perrichon et al, 2016;Katsumiti et al, 2019). Low-energy WAFs (24 h at 21 °C) produced from Arabian Light crude oil (BAL 110) and Erika heavy fuel oil (HFO, no.…”

Section: Model Equation Graphic Representation Of Equationmentioning

confidence: 80%

“…A higher surface means more sorption sites that are potential points of PAH adsorption interactions (hydrophobic and π-π binding interaction) onto the MPs (Fu et al, 2021). These adsorption interactions are more labile than absorption interaction due to the porosity of the material that could account in higher extent for sorbed B(a) P in larger particles (Katsumiti et al, 2019;Lin et al, 2019;Yu et al, 2021). Thus, higher B(a)P desorption could be expected from the small MPs (Fred-Ahmadu et al, 2020) during centrifugation and cleaning.…”

Section: Model Equation Graphic Representation Of Equationmentioning

confidence: 99%

Sorption of benzo(a)pyrene and of a complex mixture of petrogenic polycyclic aromatic hydrocarbons onto polystyrene microplastics

Martínez-Álvarez

Ménach

Devier

et al. 2022

Front. Environ. Chem.

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Microplastics (MPs) largely occur in aquatic ecosystems due to degradation of larger plastics or release from MP-containing products. Due to the hydrophobic nature and large specific surface of MPs, other contaminants, such as polycyclic aromatic hydrocarbons (PAHs), can potentially sorb onto MPs. Several studies have addressed the potential impact of MPs as vectors of PAHs for aquatic organisms. Therefore the role of MPs as sorbents of these compounds should be carefully investigated. The present study aimed to determine the sorption capacity of benzo(a)pyrene (B(a)P), as a model pyrolytic PAH, to polystyrene (PS) MPs of different sizes (4.5 and 0.5 μm). In addition, the sorption of PAHs present in the water accommodated fraction (WAF) of a naphthenic North Sea crude oil to 4.5 μm MPs was also studied as a model of a complex mixture of petrogenic PAHs that could appear in oil-polluted environments. The results indicated that 0.5 μm MPs showed higher maximum sorption capacity (Qmax) for B(a)P (145–242.89 μg/g) than 4.5 μm MPs (30.50–67.65 μg/g). From the WAF mixture, naphthalene was sorbed at a higher extent than the other PAHs to 4.5 μm MPs but with weak binding interactions (Kf = 69.25 L/g; 1/n = 0.46) according to the analysis of the aqueous phase, whereas phenanthrene showed stronger binding interactions (Kf = 0.24 L/g; 1/n = 0.98) based on the analysis of the solid phase. Sorption of PAHs of the complex WAF mixture to 4.5 μm MPs was relatively limited and driven by the hydrophobicity and initial concentration of each PAH. Overall, the results indicate that sorption estimations based solely on the analysis of the aqueous phase could overestimate the capacity of MPs to carry PAHs. Therefore, controlled laboratory assays assessing the “Trojan Horse effect” of MPs for aquatic organisms should consider these findings in order to design accurate and relevant experimental procedures.

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In vitro toxicity testing in hemocytes of the marine mussel Mytilus galloprovincialis (L.) to uncover mechanisms of action of the water accommodated fraction (WAF) of a naphthenic North Sea crude oil without and with dispersant

Cited by 30 publications

References 71 publications

Antioxidant Response and Oxidative Stress in the Respiratory Tree of Sea Cucumber (Apostichopus japonicus) Following Exposure to Crude Oil and Chemical Dispersant

Antioxidant Response and Oxidative Stress in the Respiratory Tree of Sea Cucumber (Apostichopus japonicus) Following Exposure to Crude Oil and Chemical Dispersant

ColwelliaandMarinobactermetapangenomes reveal species-specific responses to oil and dispersant exposure in deepsea microbial communities

Sorption of benzo(a)pyrene and of a complex mixture of petrogenic polycyclic aromatic hydrocarbons onto polystyrene microplastics

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