In vitro Study on Regeneration of Gladiolus grandiflorus Corm Calli as Affected by Plant Growth Regulators

Giglou, Mousa Torabi; Hajieghrari, Behzad

doi:10.3923/pjbs.2008.1147.1151

Cited by 8 publications

(10 citation statements)

References 15 publications

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“…Kumar et al [20] reported maximum shoot length in MS medium supplemented with 1.0mg/l of BAP. The successful regeneration of gladiolus in-vitro with the use of BAP were also reported [21][22][23]. MS medium containing 4mg/l of IBA showed satisfactory in-vitro rooting (Fig.…”

Section: Resultsmentioning

confidence: 62%

In-vitro Response of Gladiolus (var. American Beauty) towards Plant Growth Regulators Using Cormel Explants

Devi¹,

Maitra

Mandal

et al. 2023

IJECC

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Gladiolus is one of the most potential bulbous cut flower crops cultivated widely in India due to its attractive and long lasting spikes. The plant is propagated vegetatively through corms and cormels, but due to low rate of multiplication, its cultivation is hindered. Also, it is grown through underground modified stems, they are more often attacked by soil borne diseases such as Fusarium corm rot. In-vitro propagation techniques, assumes significance, especially for securing rapid multiplication of quality planting propagules using dormant cormlet explants and media. The results of the study revealed that the MS growing medium supplemented with 2 mg/l BAP showed improved results in respect of sprouting of explants (54.46 %), days required for sprouting of explants (8.68 DAI) and shoot length at 30 (6.32 cm), 60 (12.40 cm) and 90 (13.21 cm) DAI. The in vitro regenerated gladiolus plantlets when placed in the rooting MS medium supplemented with 4 mg/l IBA showed earlier root initiation (8.31 DAI) and higher number of roots/shoot (5.37 DAI) as compared to the rest of the treatment combinations. Hardening medium consisting of garden soil + sand + vermicompost (1:1:1) showed greater survivability (50.98 %) of plantlets. Thus, the study has been initiated an efficient protocol for in-vitro propagation of gladiolus through cormlets cultured in MS media containing BAP and IBA as shooting and rooting plant growth regulators and subsequent acclimatization in garden soil + sand+ vermicompost medium.

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Section: Resultsmentioning

confidence: 62%

In-vitro Response of Gladiolus (var. American Beauty) towards Plant Growth Regulators Using Cormel Explants

Devi¹,

Maitra

Mandal

et al. 2023

IJECC

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“…For ring conformations, see: Cremer & Pople (1975). For background to Trichoderma and diterpene lactones, see, for example: De los Santos-Villalobos et al (2011); Evidente et al (2006); Hajieghrari et al (2008); Kumar et al (2012); Vinale (2009); Xie et al (2013). For the stability of the temperature controller used in the data collection, see: Cosier & Glazer (1986 Table 1 Hydrogen-bond geometry (Å , ).…”

Section: Related Literaturementioning

confidence: 98%

“…Trichoderma genus is accepted as a superior biocontrol agent of plant pathogens (Hajieghrari et al, 2008;Kumar et al, 2012). Secondary metabolites from Trichoderma fungi have been reported to inhibit the phytopathogenic growth against Colletotrichum gloeosporioides (De los Santos-Villalobos et al, 2011), Pythium irregular, Sclerotinia sclerotiorum, Rhizoctonia solani (Vinale, 2009) and Sclerotium rolfsii (Evidente et al, 2006).…”

Section: Commentmentioning

confidence: 99%

Trichodermaerin: a diterpene lactone fromTrichoderma asperellum

et al. 2014

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The title compound, C20H28O3, known as ‘trichodermaerin’ [systematic name: (4E)-4,9,15,16,16-pentamethyl-6-oxatetracyclo[10.3.1.01,10.05,9]hexadec-4-ene-7,13-dione], is a diterpene lactone which was isolated from Trichoderma asperellum. The structure has a tetracycic 6–5–7–5 ring system, with the cyclohexanone ring adopting a twisted half-chair conformation and the cyclopentane ring adopting a half-chair conformation, whereas the cycloheptene and tetrahydrofurananone rings are in chair and envelope (with the methyl-substituted C atom as the flap) conformations, respectively. The three-dimensional architecture is stabilized by C—H⋯O interactions.

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“…The colony of the test pathogen was measured after first, second, third and fourth days of the incubation. Finally, the percent (%) inhibition growth was measured in relation of growth in control plate [19].…”

Section: In Vitro Evaluation Of Trichoderma Isolates Against Test Patmentioning

confidence: 99%

Evaluation of Trichoderma as a biological control against different pathogenic bacteria and fungi

Shah¹

2020

PAB

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Recently, the use of biological control agents (BCAs) gained immense popularity in different fields of biology, especially in pathology and entomology as an alternate approach to decrease the use of synthetic chemicals, thus reducing the environmental threats potentially dangerous to humans and the environment. This study was carried out to isolate Trichoderma from farmyard manure (FYM) and rhizosphere of plants. These isolates were successfully identified macroscopically and microscopically. The biocontrol activity of these Trichoderma isolates were evaluated in vitro against pathogenic fungi (Fusarium oxysporum, Alternaria alternata) and bacteria (Pectobacterium carotovorum, Pseudomonas syringae). Biocontrol efficiency against these pathogens was performed by dual culture technique and the inhibition percentage and radial growth percentage of pathogens were calculated. In vitro results showed that Trichoderma species showed effective biocontrol activity against all tested pathogens i-e P. carotovorum, P. syringe, F. oxysporum, and A. alternata, with inhibition percentage of 91.7%, 91.7%, 80% and 75%, respectively. The least radial growth in the presence of Trichoderma isolates was shown by P. carotovorum (8.3%) and P. syringae (8.3%) followed by F. oxysporum (20%) and A. Alternata (25%). Trichoderma species played a vital role in controlling all the tested pathogens. However, their biocontrol activity against P. carotovorum and P. syringae was found to be higher than other pathogens. This study showed the potential of Trichoderma species as a biocontrol agent and it needs to be confirmed through in vivo applications in the future. This control method is safe, cheap and eco-friendly and has no hazard to human and environment.

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In vitro Study on Regeneration of Gladiolus grandiflorus Corm Calli as Affected by Plant Growth Regulators

Cited by 8 publications

References 15 publications

In-vitro Response of Gladiolus (var. American Beauty) towards Plant Growth Regulators Using Cormel Explants

In-vitro Response of Gladiolus (var. American Beauty) towards Plant Growth Regulators Using Cormel Explants

Trichodermaerin: a diterpene lactone fromTrichoderma asperellum

Evaluation of Trichoderma as a biological control against different pathogenic bacteria and fungi

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