In vitro study of Listeria monocytogenes infection to murine primary and human transformed B cells

Menon, A. Nirmala; Shroyer, Mindy; Wampler, Jennifer L.; Chawan, C. B.; Bhunia, Arun K.

doi:10.1016/s0147-9571(02)00039-5

Cited by 33 publications

(32 citation statements)

References 59 publications

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“…54 Previously, we have shown Listeria-mediated apoptotic cell death of Ped-2E9 cell, B-cell origin, 54 which is verified further in this study ( Figure 7). As revealed by the SEM images (Figure 6), the cell membrane damage of gel-encapsulated Ped-2E9 cells caused by bacterial cells or toxins show close similarity with our earlier studies, 47,54 indicating that, the cellular and B cells in gel as biosensors P Banerjee et al physiological characteristics of both the Ped-2E9 cells and the pathogens remained unaltered in a collagen gel microenvironment. This finding is particularly important as the viability of mammalian cells after immobilization in biocompatible matrices is the key factor for cell-based assays.…”

Section: Discussionsupporting

confidence: 87%

A novel and simple cell-based detection system with a collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins

Banerjee

Lenz

Robinson

et al. 2008

Laboratory Investigation

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Cell-based biosensors (CBBs) are becoming important tools for biosecurity applications and rapid diagnostics in food microbiology for their unique capability of detecting physiologically hazardous materials. A multi-well plate-based biosensor containing B-cell hybridoma, Ped-2E9, encapsulated in type I collagen matrix, was developed for rapid detection of viable cells of pathogenic Listeria, the toxin listeriolysin O, and the enterotoxin from Bacillus species. This sensor measures the alkaline phosphatase release from infected Ped-2E9 cells colorimetrically. Pathogenic L. monocytogenes cells and toxin preparations from L. monocytogenes or B. cereus showed cytotoxicity ranging from 24 to 98% at 3-6 h postinfection. In contrast, nonpathogenic L. innocua (F4247) and B. subtilis induced minimal cytotoxicity, ranging only 0.4-7.6%. Laser scanning cytometry and cryo-nano scanning electron microscopy confirmed the live or dead status of the infected Ped-2E9 cells in gel matrix. This paper presents the first example of a cell-based sensing system using collagen-encapsulated mammalian cells for rapid detection of pathogenic bacteria or toxin, and demonstrates a potential for onsite use as a portable detection system.

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Section: Discussionsupporting

confidence: 87%

A novel and simple cell-based detection system with a collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins

Banerjee

Lenz

Robinson

et al. 2008

Laboratory Investigation

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“…Past reports indicate that infection by a variety of bacteria can cause B cell death (45,46), yet we observed that N. gonorrhoeae infection instead supports primary human B cell viability and promotes their proliferation, even decreasing cell death that normally occurs during in vitro culture of freshly isolated human B cells. In other cell types, both protection from (47,48) and promotion of (49) cell death have been observed upon infection with N. gonorrhoeae.…”

Section: Discussioncontrasting

confidence: 52%

“…Considering that other bacteria, including E. coli used in this study and Listeria monocytogenes (45) and Francisella tularensis (46) in previous work, do not elicit a similar response, this appears to be a specific effect of the gonococci rather than being a prototypical immune response. Indeed, it is intriguing to speculate that N. gonorrhoeae may promote the specific expansion of IgM memory B cells to amplify the production of nonspecific Ig in a manner that negatively affects specific immunity in infected individuals.…”

Section: Discussionmentioning

confidence: 45%

Vigorous Response of Human Innate Functioning IgM Memory B Cells upon Infection by Neisseria gonorrhoeae

Ostrowski

Gray-Owen

2012

The Journal of Immunology

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Neisseria gonorrhoeae, the cause of the sexually transmitted infection gonorrhea, elicits low levels of specific Ig that decline rapidly after the bacteria are cleared. Reinfection with the same serovar can occur, and prior gonococcal infection does not alter the Ig response upon subsequent exposure, suggesting that protective immunity is not induced. The mucosal Ig response apparent during gonorrhea does not correlate with that observed systemically, leading to a suggestion that it is locally generated. In considering whether N. gonorrhoeae directly influences B cells, we observed that gonococcal infection prolonged viability of primary human B cells in vitro and elicited robust activation and vigorous proliferative responses in the absence of T cells. Furthermore, we observed the specific expansion of IgD+CD27+ B cells in response to gonococcal infection. These cells are innate in function, conferring protection against diverse microbes by producing low-affinity, broadly reactive IgM without inducing classical immunologic memory. Although gonococcal infection of B cells produced small amounts of gonococcal-specific IgM, IgM specific for irrelevant Ags were also produced, suggesting a broad, polyspecific Ig response. The gonococci were effectively bound and engulfed by B cells. TLR9-inhibitory CpGs blocked B cell responses, indicating that intracellular bacterial degradation allows for innate immune detection within the phagolysosome. To our knowledge, this is the first report of a bacterial pathogen having specific affinity for the human IgM memory B cells, driving their potent activation and polyclonal Ig response. This unfocused T-independent response explains the localized Ig response that occurs, despite an absence of immunologic memory elicited during gonorrhea.

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“…Apoptosis in response to infection with whole, viable L. monocytogenes has been described in hepatocytes [328][329][330], epithelial cells [128], CD4 + T cells, CD8 + T cells, and B lymphocytes [125,130,134], neurons, Purkinje cells [127], dendritic cells [124,331], and natural killer (NK) cells [129] but not in macrophages [8,124,332]. Whether intracellular Listeriae actively impede PCD in macrophages to preserve their intracellular niche is an interesting and unresolved question [333].…”

Section: Streptococcus Sppmentioning

confidence: 99%

“…Listeriolysin O promotes PCD in T cells [27,123] and dendritic cells [124], thereby negating the need for bacterial internalization for PCD of the host cell. A recent study by Menon et al (2003) implicated listeriolysin O as the major virulence factor directing PCD in B cells [125]. The PCD activity of listeriolysin O, like that of S. aureus α-toxin [78] and S. pyogenes streptolysin O, is dose dependent.…”

Section: Streptococcus Sppmentioning

confidence: 99%

Regulation of Apoptosis by Gram-Positive Bacteria: Mechanistic Diversity and Consequences for Immunity

Ulett

Adderson²

2006

CIR

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Apoptosis, or programmed cell death (PCD), is an important physiological mechanism, through which the human immune system regulates homeostasis and responds to diverse forms of cellular damage. PCD may also be involved in immune counteraction to microbial infection. Over the past decade, the amount of research on bacteria-induced PCD has grown tremendously, and the implications of this mechanism on immunity are being elucidated. Some pathogenic bacteria actively trigger the suicide response in critical lineages of leukocytes that orchestrate both the innate and adaptive immune responses; other bacteria proactively prevent PCD to benefit their own survival and persistence. Currently, the microbial virulence factors, which represent the keys to unlocking the suicide response in host cells, are a primary focus of this field. In this review, we discuss these bacterial "apoptosis regulatory molecules" and the apoptotic events they either trigger or prevent, the host target cells of this regulatory activity, and the possible ramifications for immunity to infection. Gram-positive pathogens including Staphylococcus, Streptococcus, Bacillus, Listeria, and Clostridia species are discussed as important agents of human infection that modulate PCD pathways in eukaryotic cells.

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In vitro study of Listeria monocytogenes infection to murine primary and human transformed B cells

Cited by 33 publications

References 59 publications

A novel and simple cell-based detection system with a collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins

A novel and simple cell-based detection system with a collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins

Vigorous Response of Human Innate Functioning IgM Memory B Cells upon Infection by Neisseria gonorrhoeae

Regulation of Apoptosis by Gram-Positive Bacteria: Mechanistic Diversity and Consequences for Immunity

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