In vitro sliding of actin filaments labelled with single quantum dots

Månsson, Alf; Sundberg, Mark; Balaz, Martina; Bunk, Richard; Nicholls, Ian A.; Omling, P.; Tågerud, Sven; Montelius, Lars

doi:10.1016/j.bbrc.2003.12.133

Cited by 132 publications

(108 citation statements)

References 30 publications

Supporting

Mentioning

108

Contrasting

Order By: Relevance

“…Avoiding functionalization chemistry altogether, some researchers have used electrostatic interactions between NCs and charged adapter molecules, or proteins modified to incorporate charged domains 30,31 . For instance, biotinylated or streptavidin-(SAv) coated NCs can be used in combination with SAv-functionalized or biotinylated proteins or antibodies 1,9,17,23,[32][33][34][35] . Using an antibody against a specific target, and a biotinylated secondary antibody, itself bound to a SAv-coated NC, any type of target can be labeled using a three-layer approach 9,34 .…”

Section: Introductionmentioning

confidence: 99%

Peptide-coated semiconductor nanocrystals for biomedical applications

et al. 2005

View full text Add to dashboard Cite

We have developed a new functionalization approach for semiconductor nanocrystals based on a single-step exchange of surface ligands with custom-designed peptides. This peptide-coating technique yield small, monodisperse and very stable water-soluble NCs that remain bright and photostable. We have used this approach on several types of core and core-shell NCs in the visible and near-infrared spectrum range and used fluorescence correlation spectroscopy for rapid assessment of the colloidal and photophysical properties of the resulting particles. This peptide coating strategy has several advantages: it yields probes that are immediately biocompatible; it is amenable to improvements of the different properties (solubilization, functionalization, etc) via rational design, parallel synthesis, or molecular evolution; it permits the combination of several functions on individual NCs. These functionalized NCs have been used for diverse biomedical applications. Two are discussed here: single-particle tracking of membrane receptor in live cells and combined fluorescence and PET imaging of targeted delivery in live animals.

show abstract

Section: Introductionmentioning

confidence: 99%

Peptide-coated semiconductor nanocrystals for biomedical applications

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Quantum dots (QD) are semiconductor nanocrystals which have been coupled to biomolecules, such as transferrin (4,18), immunoglobulin G (IgG) (4), biotin (3), streptavidin (23,42), avidin (11,16), nucleic acids (8,38,39), peptides (2), serotonin (31), adenine (17), adenine monophosphate (17), and wheat germ agglutinin (17,18). These conjugates are luminescent probes that bind with specificity and sensitivity to a variety of targets (IgG, antigens, glycoproteins, nucleic acid sequences, and receptors).…”

mentioning

confidence: 99%

Use of Quantum Dot Luminescent Probes To Achieve Single-Cell Resolution of Human Oral Bacteria in Biofilms

Chalmers

Palmer

Du‐Thumm

et al. 2007

Appl Environ Microbiol

View full text Add to dashboard Cite

Oral biofilms are multispecies communities, and in their nascent stages of development, numerous bacterial species engage in interspecies interactions. Better insight into the spatial relationship between different species and how species diversity increases over time can guide our understanding of the role of interspecies interactions in the development of the biofilms. Quantum dots (QD) are semiconductor nanocrystals and have emerged as a promising tool for labeling and detection of bacteria. We sought to apply QD-based primary immunofluorescence for labeling of bacterial cells with in vitro and in vivo biofilms and to compare this approach with the fluorophore-based primary immunofluorescence approach we have used previously. To investigate QD-based primary immunofluorescence as the means to detect distinct targets with single-cell resolution, we conjugated polyclonal and monoclonal antibodies to the QD surface. We also conducted simultaneous QD conjugate-based and fluorophore conjugate-based immunofluorescence and showed that these conjugates were complementary tools in immunofluorescence applications. Planktonic and biofilm cells were labeled effectively by considering two factors: the final nanomolar concentration of QD conjugate and the amount of antibody conjugated to the QD, which we define as the degree of labeling. These advances in the application of QD-based immunofluorescence for the study of biofilms in vitro and in vivo will help to define bacterial community architecture and to facilitate investigations of interactions between bacterial species in these communities.Quantum dots (QD) are semiconductor nanocrystals which have been coupled to biomolecules, such as transferrin (4, 18), immunoglobulin G (IgG) (4), biotin (3), streptavidin (23, 42), avidin (11, 16), nucleic acids (8, 38, 39), peptides (2), serotonin (31), adenine(17), adenine monophosphate (17), and wheat germ agglutinin (17, 18). These conjugates are luminescent probes that bind with specificity and sensitivity to a variety of targets (IgG, antigens, glycoproteins, nucleic acid sequences, and receptors). Unlike traditional fluorophore fluorescence, QD luminescence is photostable and size tunable, with narrow, symmetric emission spectra and broad continuous excitation, allowing excitation of multiple QD with a single wavelength (3,4,12,18,42). These properties make QD very attractive luminescent labels for biological applications.In the last decade, significant advances have been made with eukaryotic applications of QD conjugates; however, bacteriological applications are few. The first use of QD for bacterial labeling was reported by Kloepfer et al. in 2003 (18). QD have since been used for labeling, detection, and quantification of Mycobacterium bovis bacillus Calmette-Guérin (25), Escherichia coli O157:H7 (33), and Salmonella enterica serovar Typhimurium (40) and recently for the simultaneous detection of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium (41). The limit and speed for QD-based detection of pathoge...

show abstract

“…[95] This study, which unfortunately was not followed up, is important because it demonstrates the benefits of using self-aligned nano-tracks in terms of both directionality and amplification of force. In addition, the transport of micro-objects has been demonstrated for (i) myosin-coated (e.g., magnetic) beads walking on actin bundles of Nitella [147]; (ii) gelsolin-functionalized 40 nm gold nanoparticles attached at one end of actin filaments [148] ; (iii) transportation of quantum dots using actin filaments, [15] and (iv) HMM-functionalized beads travelling on actin bundles self-assembled in microfabricated channels. [50] The kinesin-microtubule system was also used for the (i) transport, rotation, and flip-over of kinesin-powered micro-chips made of silicon along flow-aligned microtubules immobilized on the surface of a flow cell, [126] (ii) formation of membrane tubes and tubular networks by lipid giant unilamellar vesicles, decorated with kinesin-functionalized polystyrene beads, [149] (iii) capture and transport of streptavidin-coated beads by biotindecorated microtubules, [141,150] and (iv) transport of quantum dots linked to microtubules [145] and also to kinesin for imaging single molecule movement.…”

Section: Nanomechanical Devicesmentioning

confidence: 99%

“…The past four decades have witnessed extensive investigations into the molecular mechanisms that underpin the operation of these motors, which are central to biology. These fundamental investigations have been (i) experimental-aided by advances in molecular imaging, single molecule manipulation techniques, such as optical trapping, and development of motility assays, [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] and (ii) theoretical-analyzing and attempting to explain the solely understanding their mechanisms per se. These devices are hybrids: consisting of naturally evolved protein motors, biochemically interfaced and housed in glass or polymeric microstructures, possibly with external (fluidic or EM) control of motility.…”

Section: Introductionmentioning

confidence: 99%

Protein Linear Molecular Motor-Powered Nanodevices

Bakewell

Nicolau

2007

Aust. J. Chem.

View full text Add to dashboard Cite

Myosin-actin and kinesin-microtubule linear protein motor systems and their application in hybrid nanodevices are reviewed. Research during the past several decades has provided a wealth of understanding about the fundamentals of protein motors that continues to be pursued. It has also laid the foundations for a new branch of investigation that considers the application of these motors as key functional elements in laboratory-on-a-chip and other micro/nanodevices. Current models of myosin and kinesin motors are introduced and the effects of motility assay parameters, including temperature, toxicity, and in particular, surface effects on motor protein operation, are discussed. These parameters set the boundaries for gliding and bead motility assays. The review describes recent developments in assay motility confinement and unidirectional control, using micro-and nano-fabricated structures, surface patterning, microfluidic flow, electromagnetic fields, and self-assembled actin filament/microtubule tracks. Current protein motor assays are primitive devices, and the developments in governing control can lead to promising applications such as sensing, nano-mechanical drivers, and biocomputation.

show abstract

In vitro sliding of actin filaments labelled with single quantum dots

Cited by 132 publications

References 30 publications

Peptide-coated semiconductor nanocrystals for biomedical applications

Peptide-coated semiconductor nanocrystals for biomedical applications

Use of Quantum Dot Luminescent Probes To Achieve Single-Cell Resolution of Human Oral Bacteria in Biofilms

Protein Linear Molecular Motor-Powered Nanodevices

Contact Info

Product

Resources

About