In Vitro Selection of the RNA Aptamer against the Sialyl Lewis X and Its Inhibition of the Cell Adhesion

Jeong, Sunjoo; Eom, Tae-Yeon; Kim, Se-Jin; Lee, Seong-Wook; Yu, Jaehoon

doi:10.1006/bbrc.2001.4327

Cited by 72 publications

(39 citation statements)

References 31 publications

(19 reference statements)

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“…The biological activity of such aptamers have been shown in in vitro assays where RNA antagonists inhibited selectin-dependent adhesion (17,24). Wang et al (25) selected RNA aptamers that bind to infectious human cytomegalovirus and inhibit viral infection in vitro, showing the feasibility of the SELEX technique for the evolution of novel compounds that protect cells against infection by pathogens.…”

Section: Ref 12)mentioning

confidence: 99%

In Vitro Selection of RNA Aptamers That Bind to Cell Adhesion Receptors of Trypanosoma cruzi and Inhibit Cell Invasion

Ulrich¹,

Magdesian²,

Alves³

et al. 2002

Journal of Biological Chemistry

145

121

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Trypanosoma cruzi causing Chagas' disease needs to invade host cells to complete its life cycle. Macromolecules on host cell surfaces such as laminin, thrombospondin, heparan sulfate, and fibronectin are believed to be important in mediating parasite-host cell adhesions and in the invasion process of the host cell by the parasite. The SELEX technique (systematic evolution of ligands by exponential enrichment) was used to evolve nuclease-resistant RNA ligands (aptamer ‫؍‬ to fit) that bind with affinities of 40 -400 nM to parasite receptors for the host cell matrix molecules laminin, fibronectin, thrombospondin, and heparan sulfate. After eight consecutive rounds of in vitro selection four classes of RNA aptamers based on structural similarities were isolated and sequenced. All members of each class shared a common sequence motif and competed with the respective host cell matrix molecule that was used for displacement during the selection procedure. RNA pools following seven and eight selection rounds as well as individual aptamers sharing consensus motifs were active in inhibiting invasion of LLC-MK 2 monkey kidney cells by T. cruzi in vitro.

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Section: Ref 12)mentioning

confidence: 99%

In Vitro Selection of RNA Aptamers That Bind to Cell Adhesion Receptors of Trypanosoma cruzi and Inhibit Cell Invasion

Ulrich¹,

Magdesian²,

Alves³

et al. 2002

Journal of Biological Chemistry

145

121

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“…5. So far, several aptamers have been reported using various types of carbohydrate recognition, including monosaccharides (D-galactose, D-glucose, and D-mannose), 64 disaccharides (cellobiose), 65 oligosaccharides (sialylactose, sialyl Lewis X (sLe X Þ), 66,67 polysaccharides (cellulose, chitin, and curdlan), 68,69 and carbohydrates from glycoproteins (¯brinogen). 70 The detailed and comprehensive introduction can refer to the related review.…”

Section: Small Biological Moleculesmentioning

confidence: 99%

Super-resolution imaging in glycoscience: New developments and challenges

Chen

Tong

Wang

2016

J. Innov. Opt. Health Sci.

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Carbohydrates on cell surfaces play a crucial role in a wide variety of biological processes, including cell adhesion, recognition and signaling, viral and bacterial infection, in°ammation and metastasis. However, owing to the large diversity and complexity of carbohydrate structure and nongenetically synthesis, glycoscience is the least understood¯eld compared with genomics and proteomics. Although the structures and functions of carbohydrates have been investigated by various conventional analysis methods, the distribution and role of carbohydrates in cell membranes remain elusive. This review focuses on the developments and challenges of super-resolution imaging in glycoscience through introduction of imaging principle and the available°uorescent probes for super-resolution imaging, the labeling strategies of carbohydrates, and the recent applications of super-resolution imaging in glycoscience, which will promote the super-resolution imaging technology as a promising tool to provide new insights into the study of glycoscience.

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“…Metastasis development occurs if tumor cells adhere to distal tissues via adhesion molecules. Such molecules include the vascular cell adhesion molecule-1 (VCAM-1) (Chauveau et al, 2007), L-Selectin (Hicke et al, 1996, P-Selectin (Jenison et al, 1998), as well as the lectin Sialyl Lewis X (Jeong et al, 2001). Treatment with antagonists to these adhesion molecules could therefore inhibit the development of metastasis.…”

Section: Adhesion Moleculesmentioning

confidence: 99%

“…Aptamers specific to these targets have been developed. The ability to block cellular adhesion in vitro has been demonstrated for the Pselectin (Jenison et al, 1998) and Sialyl Lewis X aptamers (Jeong et al, 2001), whereas the function of the L-Selectin aptamer has been validated in vivo (Hicke et al, 1996) (Table 4). …”

Section: Adhesion Moleculesmentioning

confidence: 99%