In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenes

Liu, Guoqing; Lian, Ying-qi; Gao, Chao; Yu, Xiaofeng; Zhu, Ming–Sheng; Kai, Zong; Chen, Xue-Jiao; Yan, Yi

doi:10.1016/s2095-3119(14)60766-8

Cited by 16 publications

(10 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The best candidate MRE reported to have a K d value of 60.01 nM and had high specificity. A fluorescent based detection assay was developed to enable the observation of binding between the MRE and target bacteria using fluorescent microscope, but the LOD was not reported [ 60 ].…”

Section: In Vitro Selection Of Ssdna Molecular mentioning

confidence: 99%

Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications

Hong¹,

Sooter²

2015

BioMed Research International

View full text Add to dashboard Cite

Molecular recognition elements (MREs) can be short sequences of single-stranded DNA, RNA, small peptides, or antibody fragments. They can bind to user-defined targets with high affinity and specificity. There has been an increasing interest in the identification and application of nucleic acid molecular recognition elements, commonly known as aptamers, since they were first described in 1990 by the Gold and Szostak laboratories. A large number of target specific nucleic acids MREs and their applications are currently in the literature. This review first describes the general methodologies used in identifying single-stranded DNA (ssDNA) aptamers. It then summarizes advancements in the identification and biosensing application of ssDNA aptamers specific for bacteria, viruses, their associated molecules, and selected chemical toxins. Lastly, an overview of the basic principles of ssDNA aptamer-based biosensors is discussed.

show abstract

Section: In Vitro Selection Of Ssdna Molecular mentioning

confidence: 99%

Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications

Hong¹,

Sooter²

2015

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Furthermore, these conventional SELEX procedures have been used in the detection of various pathogens such as Vibrio parahaemolyticus ( Hamula et al, 2011 ), Salmonella typhimurium ( Duan et al, 2012 ), Listeria monocytogenes ( Duan et al, 2013 ). These aptamers to a single bacterial species can be created within 10 rounds of selection, while those to multiple bacterial species also can be achieved within 20 rounds of selection as shown by the aptamer selection scheme in Figure 2 ( Liu G.Q. et al, 2014 ).…”

Section: Selection Of Aptamers Against Bacterial Pathogensmentioning

confidence: 99%

Aptamer-Based Technologies in Foodborne Pathogen Detection

Teng

Fang

et al. 2016

Front. Microbiol.

View full text Add to dashboard Cite

Aptamers are single stranded DNA or RNA ligands, which can be selected by a method called systematic evolution of ligands by exponential enrichment (SELEX); and they can specifically recognize and bind to their targets. These unique characteristics of aptamers offer great potentials in applications such as pathogen detection and biomolecular screening. Pathogen detection is the critical means in detecting and identifying the problems related to public health and food safety; and only the rapid, sensitive and efficient detection technologies can enable the users to make the accurate assessments on the risks of infections (humans and animals) or contaminations (foods and other commodities) caused by various pathogens. This article reviews the development in the field of the aptamer-based approaches for pathogen detection, including whole-cell SELEX and Genomic SELEX. Nowadays, a variety of aptamer-based biosensors have been developed for pathogen detection. Thus, in this review, we also cover the development in aptamer-based biosensors including optical biosensors for multiple pathogen detection by multiple-labeling or label-free models such as fluorescence detection and surface plasmon resonance, electrochemical biosensors and lateral chromatography test strips, and their applications in pathogen detection and biomolecular screening. While notable progress has been made in the field in the last decade, challenges or drawbacks in their applications such as pathogen detection and biomolecular screening remain to be overcome.

show abstract

“…[91], Listeria sp., and Salmonella sp. are also available [92,93]. Aptamers 5'-TTTGGTCCTTGTCTT ATGTCCAGAATGC-TATGGCGGCGTCACCCGACGGG-3' and 5'-GACTTGAATTATA-CA GATTTCTCCTACTGGGATAGGTGGATTAT-3' sequences were used in salmonella serovars detection and showed high detection affinity.…”

Section: Application Of Aptamer In Food Safetymentioning

confidence: 99%

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

Ndikubwimana¹

2015

J Bioprocess Biotech

View full text Add to dashboard Cite

In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenes

Cited by 16 publications

References 24 publications

Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications

Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications

Aptamer-Based Technologies in Foodborne Pathogen Detection

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

Contact Info

Product

Resources

About