In vitro selection of autochthonous lactic acid bacterium from clownfish
            <i>Amphiprion ocellaris</i>

Paixão, Peterson Emmanuel Guimarães; Couto, Márcia Valéria Silva do; Sousa, Natalino Costa; Abe, Higo Andrade; Dias, Joel Artur Rodrigues; Meneses, Juliana Oliveira; Cunha, Fernanda dos Santos; Mouriño, J.L.P.; Martins, Maurício Laterça; Fujimoto, Rodrigo Yudi

doi:10.1111/are.14396

Cited by 5 publications

(21 citation statements)

References 21 publications

(38 reference statements)

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“…The bacterial growth rate undergoes alterations because of changes in chemical and osmotic aspects in the intestine (Erkkilä and Petäjä, 2000). In the scientific literature, E. faecium demonstrates large resistance, above 60% at an NaCl concentration of 3% (Dias et al, 2019;Paixão et al, 2020) corroborating the present values for piauçu.…”

Section: Discussionsupporting

confidence: 88%

“…Discs with a diameter of 0.8cm were removed from petri plates containing acid lactic bacteria and placed on petri plates containing Tryptone Soya Agar (TSA) previously inoculated with Aeromonas hydrophila, Aeromonas caviae, Aeromonas jandaei, Pseudomonas aeruginosa, and Streptococcus agalactiae. A positive control without probiotic bacteria, containing only antibiotic (oxytetracycline at 3 mg/L), was used to compare the results according to Vieira et al (2013) and Paixão et al (2020). After incubation (48 hours at 35ºC), the inhibition halo (mm) against the pathogen was determined.…”

Section: Formentioning

confidence: 99%

“…The bacterium with better performance regarding probiotic use was identified as the species level for method MALDI-TOF-MS (matrix-assisted laser desorption ionization time-of-flight mass spectrometry) using the molecular weight of ribosomal proteins with laser shots at a wavelength of 260-337 nm. Scores ≥1.7 (Paixão et al, 2020;Sousa et al, 2019).…”

Section: Formentioning

confidence: 99%

“…In vitro assays can aid in the selection of autochthonous bacteria with probiotic potential, determining their survival in different physiological conditions as well as their inhibitory capacity against pathogens (Dias et al, 2019;Pereira et al, 2019;Paixão et al, 2020;Qi et al, 2020;Sousa et al, 2019). Some studies have reported positive results for both in vitro and vivo assays using autochthonous probiotic bacteria in aquaculture, such as Lactobacillus spp.…”

Section: Introductionmentioning

confidence: 99%

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In vitro selection of autochthonous bacterium with probiotic potential for the neotropical fish piauçu Megaleporinus microcephalus

Barros

Silva

Dias

et al. 2022

Arq. Bras. Med. Vet. Zootec.

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The study aimed to isolate, identify, and apply in vitro tests on bacteria with autochthonous probiotic potential isolated from fifteen healthy specimens of Megaleporinus macrocephalus. The strains were selected from the intestinal tract of fish and inoculated in the Petri plate containing Sharp Man Rogosa Agar (MRS) for (48 hours at 35ºC). They were isolated based on a test of catalase, Gram stain, tolerance to different gradients NaCl (1, 2 and 3%), pH (4, 5, 6, 8 and 9) values and bile salts (2.5 and 5%), in addition to the inhibition zone against pathogens. Of the 42 strains isolated, ST1 and ST9 had higher values (p<0.05) for total viable cells (31.80±0.07 and 32.51±0.05 CFU/mL × 108) respectively. In the resistance tests, strains ST1 and ST9 presented the best results, with emphasis on ST9 in the gradients of pH, high values of bile salts and larger inhibition zones against Aeromonas hydrophila and Aeromonas jandaei. The strains with the best results in the tests, ST1 and ST9, were identified by the MALDI-TOF-MS method as Enterococcus faecium. Thus, the isolated E. faecium bacteria, may be recommended as for probiotic use in farming the M. macrocephalus.

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Section: Discussionsupporting

confidence: 88%

Section: Formentioning

confidence: 99%

Section: Formentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro selection of autochthonous bacterium with probiotic potential for the neotropical fish piauçu Megaleporinus microcephalus

Barros

Silva

Dias

et al. 2022

Arq. Bras. Med. Vet. Zootec.

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“…The in vitro test was performed following the methods described by Vieira et al (2013) and Paixão et al (2019). The probiotics were cultivated in MRS broth culture medium and 100 μl (×10 9 CFU·ml −1 ) of each treatment was inoculated in MRS agar medium and incubated at 35°C for 48 h. After bacterial growth, 0.8 cm diameter discs were removed from the agar plates, superimposed on plates with Mueller Hinton Agar medium containing Aeromonas hydrophila , and incubated at 35°C for 48 h for further analysis of the inhibition halo (mm).…”

Section: Methodsmentioning

confidence: 99%

Comparative effects of autochthonous single‐strain and multi‐strain probiotics on the productive performance and disease resistance in Colossoma macropomum (Cuvier, 1818)

Dias

Alves

Barros

et al. 2022

Aquaculture Research

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This study evaluated the effects of autochthonous single‐strain and multi‐strain (mix) probiotics on the zootechnical performance and sanitary conditions of juvenile neotropical fish. Fingerlings of tambaqui (Colossoma macropomum) were fed three diets of single‐strain probiotics (two Enterococcus faecium strains and one Bacillus cereus autochthonous strain) and a multi‐strain probiotic diet (a mix of three probiotic strains) for 120 days. After dietary supplementation, 90 tambaquis were intraperitoneally injected with Aeromonas hydrophila at a concentration of 1.8 × 108 CFU·g−1. Clinical signs of disease, infectious intensity and accumulated mortality rates were evaluated. The use of diets containing probiotics, regardless of strain, enhanced productive performance from 90 experimental days (p < 0.05). The multi‐strain probiotics reduced the presence of potentially pathogenic bacteria in the intestine. Fish fed probiotics showed improved resistance to A. hydrophila infection, while the diets containing B. cereus (an autochthonous probiotic) and multi‐strain probiotic promoted the lowest mortality rates and higher leucocyte and thrombocyte counts (p < 0.05). The results revealed that the use of probiotics as a single autochthonous or multi‐strain probiotic enhanced fish growth, prevented dysbiosis and increased disease resistance.

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Autochthonous bacterium Lactobacillus plantarum as probiotic supplementation for productive performance and sanitary improvements on clownfish Amphiprion ocellaris

et al. 2020

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In vitro selection of autochthonous lactic acid bacterium from clownfish Amphiprion ocellaris

Cited by 5 publications

References 21 publications

In vitro selection of autochthonous bacterium with probiotic potential for the neotropical fish piauçu Megaleporinus microcephalus

In vitro selection of autochthonous bacterium with probiotic potential for the neotropical fish piauçu Megaleporinus microcephalus

Comparative effects of autochthonous single‐strain and multi‐strain probiotics on the productive performance and disease resistance in Colossoma macropomum (Cuvier, 1818)

Autochthonous bacterium Lactobacillus plantarum as probiotic supplementation for productive performance and sanitary improvements on clownfish Amphiprion ocellaris

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