In vitro Selection: A Novel Source of Resistance to Sclerotinia Stem Rot in Canola (Brassica napus L.)

<i>In vitro </i>technique for selecting onion for white rot disease resistance

“…A higher OA concentration (20 mM) completely inhibited germination of all onion cultivars. Our results are in agreement with Chamandoosti (2009) who found that 20 mM of OA had a lethal effect on canola and all the explants died, and that no callusing, rooting and shooting occurred. Callus induction was significantly affected by OA, and the extent of the response was OA concentration dependent (Table 2).…”

“…Oxalic acid has been used for in vitro selection studies in many important crops affected by Sclerotium fungi (Chamandoosti et al, 2006;Chamandoosti, 2007;Chamandoosti, 2009). Chamandoosti (2009) reported that the addition of 0.20 mM OA was needed for in vitro canola selection media in order to produce canola plants resistant to Sclerotinia sclerotiomm.…”

“…Chamandoosti (2009) reported that the addition of 0.20 mM OA was needed for in vitro canola selection media in order to produce canola plants resistant to Sclerotinia sclerotiomm. Chamandoosti (2009) reported that the mean diameter of calli and mean number of regenerated roots and shoots of hypocotyl explants of 7 days old canola seedling growing on 0.20 mM OA was higher than in OA-free medium. It is possible that in these experiments the gradual adaptation of hypocotyl explants in the toxic callusing culture medium and subsequent transfer of obtained calli to organogenesis medium generated tolerant or resistant whole plants.…”

“…Oxalate-deficient S. sclerotiorum, which are an important source of inoculum in the field and in in vitro cultivation is unable to produce oxalate during the infection of petals (Rejanel and Stotz, 2004;Chamandoosti, 2009).…”