In vitro screening and evaluation of phenolic antioxidant-linked anti-hyperglycemic functions of rabbit-eye blueberry (Vaccinium ashei) cultivars

Sarkar, Dipayan; Agustinah, Widya; Woods, Floyd M.; Coneva, Elina D.; Vinson, Edgar; Shetty, Kalidas

doi:10.3233/jbr-170154

Cited by 19 publications

(18 citation statements)

References 35 publications

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“…The same was observed by Cardeñosa and co-workers, where the genotype influenced the antioxidant capacity and the content of the three groups of phenolics in blueberry fruits [ 32 ]. In accordance of Sarkar and co-workers, the genotype versus environment interactions are most critical in the in vitro anti-diabetic-relevant functionalities of blueberry bioactives [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Influence of Harvest Season and Cultivar on the Variation of Phenolic Compounds Composition and Antioxidant Properties in Vaccinium ashei Leaves

et al. 2017

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The effect of variation of harvest season and cultivar on the total phenolic content (TPC), total flavonoid content (TFC), HPLC-UV/DAD profile and antioxidant properties in Vaccinium ashei (Rabbiteye blueberry) leaves grown in Brazil was evaluated. The cultivars collected in December and March were Aliceblue, Powderblue, Climax, Bluegem and FloridaM. It was observed that leaves from March had the highest TPC values (222 ± 1 mg gallic acid equivalents/g to Aliceblue cultivar) and highest TFC values (49.8 ± 0.8 and 48.7 ± 0.7 µg rutin/g to Clímax and Powderblue cultivars, respectively). The chromatographic profile was quantitatively similar, however, the proportions of each compound were influenced by cultivar and harvest season. Chlorogenic acid and rutin were the main identified phenolic compounds, but chlorogenic acid was the most abundant in both harvest seasons. Antioxidant capacities values ranged from 5.80 ± 0.04 to 105 ± 2 µg/mL (DPPH) and 178 ± 5 to 431 ± 8 mmol Trolox/100 g (ORAC). The cultivar Bluegem by March had the highest values in both assays. The results indicate that the blueberry leaves from different cultivars and harvest seasons have different phenolic compounds content and different antioxidant capacities. In addition, the antioxidant properties demonstrated a high correlation with rutin content.

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Section: Resultsmentioning

confidence: 99%

Influence of Harvest Season and Cultivar on the Variation of Phenolic Compounds Composition and Antioxidant Properties in Vaccinium ashei Leaves

et al. 2017

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“…Moreover, the α‐amylase inhibitory activity was also found to be correlated with the antioxidant capacity of the blueberry extract (see Figure 6b) with a correlation coefficient r equal to .726. A previous study evaluated five different rabbit‐eye blueberry cultivars and showed that their α‐amylase inhibitory activity has a positive correlation with both phenolic content and antioxidant capacity (Sarkar et al., 2017). He et al obtained the highest r value (.485), among the relationships studied, for the correlation between α‐amylase inhibitory activity and total polyphenol content when investigating 22 fruit juices after in vitro gastrointestinal digestion (He et al., 2017).…”

Section: Resultsmentioning

confidence: 99%

“…(3) y = Bottom + (Top − Bottom) ∕ 1 + 10 ( logIC50 − x ) × HillSlope , F I G U R E 5 The α-amylase (a) and tyrosinase (b) activity inhibition percentage versus the log of blueberry concentration; experimental data (dot), simulation (line) F I G U R E 6 Correlations of α-amylase inhibitory activity with TPC (a) and antioxidant capacity of blueberry extract (b) a correlation coefficient r equal to .726. A previous study evaluated five different rabbit-eye blueberry cultivars and showed that their α-amylase inhibitory activity has a positive correlation with both phenolic content and antioxidant capacity (Sarkar et al, 2017). He et al obtained the highest r value (.485), among the relationships studied, for the correlation between α-amylase inhibitory activity and total polyphenol content when investigating 22 fruit juices after in vitro gastrointestinal digestion (He et al, 2017).…”

Section: Enzyme Inhibitory Activitymentioning

confidence: 99%

α‐Amylase and tyrosinase inhibitory activities, phenolic contents, and antioxidant capacities of wild and cultivated blueberries

Tian

Zang

et al. 2020

J Food Process Preserv

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The phenolic compound contents, antioxidant capacities, and α‐amylase and tyrosinase inhibitory activities of 10 wild and 19 cultivated blueberries grown in northeast China were evaluated. The results showed that the α‐amylase inhibitory activities of the blueberries represented, by 1/IC50, correlated with their total phenolic contents (TPCs). Moreover, the tyrosinase inhibitory activities correlated with the total anthocyanin contents (TACs). High‐performance liquid chromatography and electrospray mass spectrometry were conducted to determine and identify seven phenolic acids. The average caffeic acid contents of the cultivated and wild blueberries were 277.37 and 17.71 μg/g, respectively. The total phenolic, anthocyanin, and flavonoid contents were determined. Wild blueberries have higher TPCs, α‐amylase inhibitory activities, and antioxidant capacities. Cultivated blueberries have higher TACs and exhibit tyrosinase inhibitory activities similar to those of the wild ones. Practical applications This study revealed that wild blueberries have high α‐amylase inhibitory activities and are effective as natural α‐amylase inhibitors for the treatment of diabetes. In addition, wild blueberries were found to be rich in phenolics and have high antioxidant capacities. Thus, because of these properties, wild blueberry products with antidiabetic and antioxidant potential can be developed for use in biomedical and pharmaceutical industries.

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“…Tyrosinase is one of the key enzymes responsible for the biosynthesis of melanin, and its inhibition is considered the best strategy to treat epidermal hyperpigmentation problems [53]. Tyrosinase also causes browning of fruits and vegetables, leading to quicker degradation and deterioration of nutritional value [54]. The use of tyrosinase inhibitors has attracted great attention in the cosmetic and pharmaceutical industries due to their preventive effects in pigmentation disorders.…”

Section: Discussionmentioning

confidence: 99%

“…The use of tyrosinase inhibitors has attracted great attention in the cosmetic and pharmaceutical industries due to their preventive effects in pigmentation disorders. This scenario demands deliberate action involving the use of enzyme-inhibitory compounds as an effective strategy [54].…”

Section: Discussionmentioning

confidence: 99%

Metabolic fingerprinting, antioxidant characterization, and enzyme-inhibitory response of Monotheca buxifolia (Falc.) A. DC. extracts

Ali

Saleem

Mannan

et al. 2020

BMC Complement Med Ther

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Background Ethnobotanical and plant-based products allow for the isolation of active constituents against a number of maladies. Monotheca buxifolia is used by local communities due to its digestive and laxative properties, as well as its ability to cure liver, kidney, and urinary diseases. There is a need to explore the biological activities and chemical constituents of this medicinal plant. Methods In this work, the biochemical potential of M. buxifolia (Falc.) A. DC was explored and linked with its biological activities. Methanol and chloroform extracts from leaves and stems were investigated for total phenolic and flavonoid contents. Ultrahigh-performance liquid chromatography coupled with mass spectrometry (UHPLC–MS) was used to determine secondary-metabolite composition, while high-performance liquid chromatography coupled with photodiode array detection (HPLC–PDA) was used for polyphenolic quantification. In addition, we carried out in vitro assays to determine antioxidant potential and the enzyme-inhibitory response of M. buxifolia extracts. Results Phenolics (91 mg gallic-acid equivalent (GAE)/g) and flavonoids (48.86 mg quercetin equivalent (QE)/g) exhibited their highest concentration in the methanol extract of stems and the chloroform extract of leaves, respectively. UHPLC–MS analysis identified a number of important phytochemicals, belonging to the flavonoid, phenolic, alkaloid, and terpenoid classes of secondary metabolites. The methanol extract of leaves contained a diosgenin derivative and polygalacin D, while kaempferol and robinin were most abundant in the chloroform extract. The methanol extract of stems contained a greater peak area for diosgenin and kaempferol, whereas this was true for lucidumol A and 3-O-cis-coumaroyl maslinic acid in the chloroform extract. Rutin, epicatechin, and catechin were the main phenolics identified by HPLC–PDA analysis. The methanol extract of stems exhibited significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging activities (145.18 and 279.04 mmol Trolox equivalent (TE)/g, respectively). The maximum cupric reducing antioxidant capacity (CUPRAC) (361.4 mg TE/g), ferric-reducing antioxidant power (FRAP) (247.19 mg TE/g), and total antioxidant potential (2.75 mmol TE/g) were depicted by the methanol extract of stems. The methanol extract of leaves exhibited stronger inhibition against acetylcholinesterase (AChE) and glucosidase, while the chloroform extract of stems was most active against butyrylcholinesterase (BChE) (4.27 mg galantamine equivalent (GALAE)/g). Similarly, the highest tyrosinase (140 mg kojic-acid equivalent (KAE)/g) and amylase (0.67 mmol acarbose equivalent (ACAE)/g) inhibition was observed for the methanol extract of stems. Conclusions UHPLC–MS analysis and HPLC–PDA quantification identified a number of bioactive secondary metabolites of M. buxifolia, which may be responsible for its antioxidant potential and enzyme-inhibitory response. M. buxifolia can be further explored for the isolation of its active components to be used as a drug.

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In vitro screening and evaluation of phenolic antioxidant-linked anti-hyperglycemic functions of rabbit-eye blueberry (Vaccinium ashei) cultivars

Cited by 19 publications

References 35 publications

Influence of Harvest Season and Cultivar on the Variation of Phenolic Compounds Composition and Antioxidant Properties in Vaccinium ashei Leaves

Influence of Harvest Season and Cultivar on the Variation of Phenolic Compounds Composition and Antioxidant Properties in Vaccinium ashei Leaves

α‐Amylase and tyrosinase inhibitory activities, phenolic contents, and antioxidant capacities of wild and cultivated blueberries

Metabolic fingerprinting, antioxidant characterization, and enzyme-inhibitory response of Monotheca buxifolia (Falc.) A. DC. extracts

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