In Vitro Propagation of Paronychia chartacea

McKently, A. H.; Adams, James

doi:10.21273/hortsci.29.8.921

“…46.92% ranging from 34.56% to 60% only, is considered as reduced fertility leading to reduced seed set [31]. Various reports are available which suggest that natural propagation through seeds in many rare and endangered plants is limited due to many factors such as seed dormancy and pollen sterility, poor seed viability [41] and little or no seed production [27]. Recently, Bhowmik et al [1] reported comparatively very low in vivo germination of seeds for M. spathulata and M. wengeri (20% and 24%, respectively).…”

Section: Discussionmentioning

confidence: 99%

Synaptic variation derived plausible cytogenetical basis of rarity and endangeredness of endemic Mantisia spathulata Schult

Sharma

¹

,

Kumaria

²

,

Tandon

³

et al. 2011

Nucleus

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We studied meiosis in Mantisia spathulata, a rare, endangered and endemic rhizomatous horticultural herb showing synaptic variation. Most of pollen mother cells (PMCs) analyzed at either diplotene or diakinesis/metaphase I did not exhibit the expected chromosome associations of 10II, which is indicative of synaptic variation. Seventeen percent of the PMCs have shown normal meiotic pattern while 83% PMCs were with abnormal meiotic behavior of bivalents. A total of 419 bivalents and 1,162 of univalents were recorded with significantly low chiasma frequency (8.38±6.20) and terminalization coefficient of 0.82 only. We recorded in 53.33% PMCs, an anomalous distribution pattern of chromosomes, including unequal distribution and/or presence of laggards in the form of univalents/bivalents resulting in low pollen stainability. We suggest that the imbalanced meiosis with variant synaptic behavior of bivalents recorded in M. spathulata may be responsible for loss of genetic variation and considered as substantial cytogenetical factors leading to the rarity and endangeredness of the species.

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Low genetic diversity as revealed by SPAR methods possibly leads to extinction of two critically-endangered and endemic species of Mantisia

Sharma¹,

Bhowmik²,

Kumaria³

et al. 2011

Biol Plant

0

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Mantisia spathulata Schult. and M. wengeri Fischer, two critically-endangered, endemic and rare species of the genus Mantisia (Zingiberaceae), have been rediscovered from Lunglei province of Mizoram, India, after two decades. For sustainable conservation and utilization of the Mantisia species, in vitro seed and clonal propagation methods have been developed earlier by our research group and plantlets have been reintroduced to their natural habitat for species recovery. To comprehend the plausible reasons for endemism and endangeredness of both the species at DNA level, they were analyzed to assess natural genetic variation using three different polymerase chain reaction (PCR) based DNA markers viz. random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and directed amplification of minisatellite DNA regions (DAMD), both individually and cumulatively, which are popularly regarded as single primer amplification reaction (SPAR) methods. A total of 107 primers belonging to three SPARs are used which collectively endow low genetic variation (15 and 20 %, respectively) in both M. spathulata and M. wengeri. The use and efficacy of SPAR methods to reveal the natural genetic variation in Mantisia species at intra-specific level has been recorded for the first time. To impede the extinction risk of these two species of genus Mantisia, large scale conservation strategies including in situ and ex situ conservation are recommended.

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Low genetic diversity as revealed by SPAR methods possibly leads to extinction of two critically-endangered and endemic species of Mantisia

Sharma¹,

Bhowmik²,

Kumaria³

et al. 2012

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Mantisia spathulata Schult. and M. wengeri Fischer, two critically-endangered, endemic and rare species of the genus Mantisia (Zingiberaceae), have been rediscovered from Lunglei province of Mizoram, India, after two decades. For sustainable conservation and utilization of the Mantisia species, in vitro seed and clonal propagation methods have been developed earlier by our research group and plantlets have been reintroduced to their natural habitat for species recovery. To comprehend the plausible reasons for endemism and endangeredness of both the species at DNA level, they were analyzed to assess natural genetic variation using three different polymerase chain reaction (PCR) based DNA markers viz. random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and directed amplification of minisatellite DNA regions (DAMD), both individually and cumulatively, which are popularly regarded as single primer amplification reaction (SPAR) methods. A total of 107 primers belonging to three SPARs are used which collectively endow low genetic variation (15 and 20 %, respectively) in both M. spathulata and M. wengeri. The use and efficacy of SPAR methods to reveal the natural genetic variation in Mantisia species at intra-specific level has been recorded for the first time. To impede the extinction risk of these two species of genus Mantisia, large scale conservation strategies including in situ and ex situ conservation are recommended.

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In Vitro Propagation of Paronychia chartacea

Cited by 4 publications

References 5 publications

Synaptic variation derived plausible cytogenetical basis of rarity and endangeredness of endemic Mantisia spathulata Schult

Synaptic variation derived plausible cytogenetical basis of rarity and endangeredness of endemic Mantisia spathulata Schult

Low genetic diversity as revealed by SPAR methods possibly leads to extinction of two critically-endangered and endemic species of Mantisia

Low genetic diversity as revealed by SPAR methods possibly leads to extinction of two critically-endangered and endemic species of Mantisia

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