In Vitro Propagation of Highland Papayas (Carica Pubescens and C. Pentagona)

Jordán, Miguel; Velozo, J.

doi:10.17660/actahortic.1997.447.10

Cited by 3 publications

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“…Plant growth regulators are used for in vitro androgenesis in anther culture, but their effects depend on many factors, such as plant genotype, type and development of tissue, culture conditions or environment, and even endogenous and exogenous concentration or type of plant growth regulator (Moubayidin et al., ; Żur et al., ). In V. pubescens , the best efficiency for inducing somatic embryos from anthers was observed in MS medium supplemented with NAA 5.37 μM and 6‐BAP 4.44 μM (Jordan and Velozo, ). In the related species C. papaya , the best combination for somatic embryo induction was CPPU 0.04 μM and NAA 0.54 μM, with 13.8% of embryo regeneration (Rimberia et al., ); this is the same combination used in treatment 9 in the current study (Table ).…”

Section: Discussionmentioning

confidence: 99%

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Regeneration of highland papaya (Vasconcellea pubescens) from anther culture

Chong-Pérez¹,

Carrasco

Silva

et al. 2018

Appl Plant Sci

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Premise of the Study Vasconcellea pubescens is an important Caricaceae species cultivated in several countries of South America. The objective of this study was to investigate different media compositions and plant growth regulators to induce plant regeneration.MethodsAnthers were cultured in Murashige and Skoog medium with varying concentrations of naphthalene acetic acid (NAA) and 2,4‐dichlorophenoxyacetic acid (2,4‐D) plus a cytokinin (N‐(2‐chloro‐4‐pyridyl)‐N′‐phenylurea). The effect of the basal medium supplemented with auxins and cytokinins on shoot regeneration from the induced calli was also evaluated. Addition of maltose to the basal medium was also tested.ResultsThe combination of 0.54 μM NAA and 22.66 μM 2,4‐D induced the highest rate of calli formation. Regeneration via organogenesis was obtained in Murashige and Skoog and Woody Plant Medium supplemented with maltose and containing 8.88 μM 6‐benzylaminopurine, 5.71 μM indoleacetic acid, and 2.28 μM zeatin.DiscussionThe plant regeneration protocol reported here permits the development of haploid and double haploid plants that can be useful for propagation purposes, allow a better molecular understanding of the species, and facilitate the production of new cultivars.

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Section: Discussionmentioning

confidence: 99%

“…The production of haploid or double haploid plants in V. pubescens has not previously been reported. Jordan and Velozo () reported the formation of somatic embryos from anthers cultured with NAA and 6‐benzylaminopurine (6‐BAP), but these authors did not discuss the regeneration of plants from these embryos.…”

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confidence: 99%