In Vitro Propagation of Hibiscus Rosa-Sinensis (L.)

Sterilization of <i>Hibiscus rosa-sinensis</i> L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on <i>In Vitro</i> Direct Regeneration

“…Plant cells and tissues could produce some essential vitamins in vitro but at a sub-optimal level, hence an exogenous supply of vitamin is always crucial. Similar to other plant species, all vegetative propagations of H. rosa-sinensis as reported involved the complete MS organic supplementations [13,18,34]. In contrast to previous reports, the results obtained in this study (Figure 1(b)) revealed that shoots induction with myoinositol and thiamine (MT) supplementations only were achieved at a percentage higher than that with myoinositol, thiamine, and nicotinic acid (MTN).…”

Agrobacterium tumefaciens-mediated transformation of axillary bud callus of Hibiscus rosa-sinensis L. ‘Ruby’ and regeneration of transgenic plants

“…The percentage of calli producing shoots was determined after 15 days. To induce roots, regenerated shoots (5-6 cm length) were separated from the callus and transferred individually to a Rooting Medium (RO) consisting of halfstrength basal medium supplemented with 2.85 lM indole-3-acetic acid (IAA) in tubs as previously described by Airò et al (2009). All the culture media used in this work were adjusted to pH 5.7 prior to autoclaving at 121°C for 15 min and are listed in Table 1.…”

“…In vitro studies on H. rosa-sinensis have led to the development of protocols for micropropagation (Airò et al 2009;Christensen et al 2008) and to the production of virus-free plants, which is important because of the species' widely reported high susceptibility to viruses (Mao et al 2008;De Stradis et al 2008;Huang et al 2004). However, regeneration of whole plants from transformed tissue of H. rosa-sinensis has up until now been unsuccessful.…”