In Vitro Production of Sweet Peas (Lathyrus odoratus L.) via Axillary Shoots

Ochatt, Sergio; Conreux, Catherine; Jacas, L.

doi:10.1007/978-1-60327-114-1_27

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…Weber et al [ 39 ] used apical meristems explants of the interspecific hybrids originating from crosses between H. annuus and nine others species of Helianthus , and concluded that a species regeneration capacity was significantly determined by the individual genotype. Variability in the organogenic responses from the axillary buds among genotypes was observed in many other species including Rosa rugosa [ 72 ], Vigna unguiculata [ 73 ], and Lathyrus sativus [ 74 ]. The observed variation in the morphogenetic response among the six tested plants of H. verticillatus warrants future studies for the levels of endogenous PGRs in the context of genotype and stem localization.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Propagation of an Endangered Helianthus verticillatus by Axillary Bud Proliferation

Nowakowska

Pavlović

Nowicki

et al. 2020

Plants

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Helianthus verticillatus (Asteraceae), whorled sunflower, is a perennial species restricted to a few locations in the Southeastern United States. Habitat loss has caused H. verticillatus to become rare, and since 2014, it has been federally listed as an endangered species. As a part of the recovery plan for the restoration and protection of H. verticillatus, an efficient micropropagation protocol based on axillary shoot proliferation was developed. Various concentrations of 6-benzylaminopurine (BAP; 0 to 4.44 µM) were examined for their morphogenetic potential in the regeneration of six genotypes of H. verticillatus from the nodal explants derived from greenhouse-grown plants. Both the BAP concentration and genotype had significant effects on the regeneration capacity of H. verticillatus. Although the induced buds were observed on ½-strength Murashige and Skoog medium without plant growth regulators, a higher rate of induction and bud development were achieved on media with either 0.88 or 2.22 µM BAP, regardless of the genotype. Successful rooting of the induced shoots was achieved within four weeks after the transfer from the induction medium to the fresh ½-strength MS medium, but the rooting efficiency was dependent on the plant’s genetic background. Regenerated plantlets, with well-developed shoots and roots, were acclimatized successfully to greenhouse conditions with a 97% survival rate. Simple sequence repeats (SSRs) markers were employed to assess the genetic uniformity of the micropropagated plants of H. verticillatus. No extraneous bands were detected between regenerants and their respective donor plants, confirming the genetic fidelity and stability of regenerated plants. To our knowledge, the protocol developed in this study is the first such report for this endangered species.

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Section: Resultsmentioning

confidence: 99%

In Vitro Propagation of an Endangered Helianthus verticillatus by Axillary Bud Proliferation

Nowakowska

Pavlović

Nowicki

et al. 2020

Plants

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“…Wesely et al (2010) observed that MS medium supplemented with 0.5 mg/l IBA resulted in 8.3 roots per shoot of Boerhaavia diffusa. Ochatt et al (2010) demonstrated that for rooting of Lathyrus odoratus L. microshoots, they are explanted onto medium with 0.5 to 1 mg/L NAA. Abido et al (2011) stated that the highest number of roots were obtained when Mirabilis jalapa neoformed shoots were treated with NAA at 0.2 mg/l.On the other side, Shah et al (2006) reported that the highest numbers of plants of Bougainvillea spectabilis were rooted when MS medium was supplemented with 2.5 mg/l IBA and NAA 2.5 mg/l.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Propagation and Somatic Embryogenesis of Marvel of Peru (Mirabilis jalapa L.) from Nodal and Leaf Explants

2015

Alexandria Science Exchange Journal: An International Quarterly

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Nowadays, there is a tremendous demands for Mirabilis jalapa L., it is a popular ornamental landscape plant grown worldwide for the beauty of its flowers, also earned its place in herbal medicine practices around the world for traditional medicine by the people from different countries. Therefore, in vitro propagation and somatic embryogenesis of Mirabilis jalapa was achieved during both seasons of 2012 and 2013, from nodal and leaf explants on MS medium supplemented with different concentrations and combinations of NAA, KIN, IBA and 2,4-D. Respecting in vitro propagation, the highest mean number of shoots (2.67) formed per propagule were recorded on MS medium supplemented with 3.0 mg/l KIN and 0.250 mg/l NAA. Regarding to rhizogenesis stage, it is obvious that, fortifying MS-medium with a combination of both IBA at 0.00 mg/l, and NAA at 0.500 mg/l, gave the highest mean value of roots formed per propagule (6.11). On the other end, the best results of embryonic callus induction response (100%) and embrogenic callus size per propagule (++++) in whole area of leaf explant was observed on MS medium augmented with 4.0 mg/l 2,4-D. Neoformed plantlets were successfully acclimated and established in peat and sand in ratio of 1:1, and flowered successfully under field conditions.

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