In vitro production of haploid and doubled haploid plants from pollinated ovaries of maize (Zea mays)

Tang, F. A.; Tao, Yongfu; Zhao, Tianyong; Wang, Guoying

doi:10.1007/s11240-005-9017-7

Cited by 47 publications

(21 citation statements)

References 9 publications

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“…Plant regeneration via ovary culture has also been reported in several plants such as lily (Van Tuyl et al 1991), sweet potato (Ruth et al 1993), onion (Bohanec et al 1995, Luthar andBohanec 1999), sugar beet (Gurel et al 2000), maize (Tang et al 2006), coconut (Perera et al 2007) and Psoralea corylifolia (Chand and Sahrawat 2007). Although the main objective of the above studies, including the present study, was to obtain haploid plants; regeneration of diploid plants revealed the potential use of ovary explants for adventitious shoot proliferation.…”

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confidence: 95%

In vitro organogenesis and plant regeneration from unpollinated ovary cultures of Azadirachta indica

Srivastava¹,

Singh²,

Mathur³

et al. 2009

Biol. plant.

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A novel method of organogenesis in neem (Azadirachta indica A. Juss.) from unfertilized ovaries is described. The Murashige and Skoog's (MS) medium with 9 % sucrose, 1 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 5 μM 6-benzylaminopurine (BAP) was the best for callus induction from unfertilized ovaries. However, further proliferation of callus occurred better on MS medium supplemented with 0.5 μM 2,4-D either alone or in combination with 4.5 μM kinetin. Maximum shoot regeneration (78 %) was observed when calli, induced from ovaries of 4 mm size flower buds and proliferating on MS + 0.5 μM 2,4-D, were subcultured to MS medium containing 5 μM BAP. Histological analysis revealed that 4 mm sized flower bud corresponds to a 2-nucleate stage of embryo sac. The shoots were then multiplied by forced axillary branching on MS medium supplemented with 1.0 μM BAP and 250 mg dm -3 casein hydrolysate. The shoots could be rooted on ¼ strength MS medium supplemented with 0.5 μM indole-3-butyric acid (IBA) at a frequency of 79 %. Cytological analysis by root tip squash preparations revealed that all the plantlets were diploids. These plants were subsequently hardened and established in soil with transplantation rate of 81.8 %.Additional key words: adventitious shoot proliferation, auxins, callus culture, casein hydrolysate, cytokinins, embryo sac, histology, ovary culture. ⎯⎯⎯⎯Neem (Azadirachta indica A. Juss.), an evergreen tropical tree belonging to the family Meliaceae, has many important medicinal, agrochemical and economic uses. Due to highly heterozygous nature, long reproductive cycle and poor seed yield, improvement of neem by conventional methods is very limited. In this respect, tissue culture can play an important role. In vitro regeneration of neem from various vegetative tissues was published (Chaturvedi et al. 2004a, Rout 2005. Recently, some studies have described shoot regeneration from anthers/microspores (Chaturvedi et al. 2003a) and endosperm tissues (Chaturvedi et al. 2003b) of adult tree origin.Even though the main objective of the present study was to obtain haploid plants from the unfertilized ovary, regeneration of diploid plants indicated the potential use of this juvenile unfertilized ovary explant for large scale micropropagation. In contrast, the leaves, nodal and internodal segments from mature trees show recalcitrance due to accumulation of secondary metabolites.Unfertilized ovaries, obtained from closed flower buds of an adult 54-year-old neem tree, were used as explants. Ovaries were excised from flower buds of four sizes (2, 3, 4 and 5 mm) and the corresponding developmental stage of ovary was determined by paraffin sections. The flower buds were surface sterilized with 0.1 % (m/v) solution HgCl 2 for 7 min, followed by three washings in sterile-distilled-water. The ovaries were dissected out with the aid of a stereo-microscope (Nikon SMZ-645, Tokyo, Japan). Four ovaries were cultured in ⎯⎯⎯⎯

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confidence: 95%

In vitro organogenesis and plant regeneration from unpollinated ovary cultures of Azadirachta indica

Srivastava¹,

Singh²,

Mathur³

et al. 2009

Biol. plant.

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“…This technique is the female equivalent of the process described in the paragraph above, and has been applied to species including sugar beet [55,56], onion [57,58], squash [59], gerbera [60], rice [61], maize [62], niger [63] and tea [64]. Ovules have also been used as a transformation target [39].…”

Section: Ovule Culturementioning

confidence: 99%

Plant Cell Culture – Present and Future

Dunwell

2010

Plant Cell Culture

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Plant cell culture has a long history of development and exploitation that has been reviewed many times [1]. There are also several monographs and textbooks that provide detailed discussion on specific techniques and their application [2, 3]. By necessity therefore, the present review is limited in scope and, for the most part, will focus on recent publications. The various sections below each concentrate on a specific aspect of the technology; these are followed by a summary section relating to commercialization. MicropropagationMicropropagation in vitro is well established as a method to propagate [4], preserve and transport germplasm of many species including horticultural [5-7], medicinal [8, 9] and woody [10,11] plants. It also reduces the risk of moving pathogens and insects with the germplasm owing to the inherent pathogen detection capabilities of aseptic cultures. Since this technology is usually limited to the multiplication of pre-existing meristems and does not involve the regeneration of plants from single cells or tissue, it will not be discussed in detail here.

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“…Development of haploids by biotechnological methods leads to the creation of homozygous genotypes in one generation; therefore a double haploid system is an extremely valuable breeding tool (Wedzony et al, 2009). Plant regeneration in vitro from isolated ovary has been achieved in some crops such as onion (Kamštaitytė, Stanys, 2002), maize (Tang et al, 2006), sugar beet (Gurel et al, 2000), wheat (Sibi et al, 2001). Lately, the overall plant regeneration frequency in flax from anther has been significantly increased by the modification of medium composition (Chen, Dribnenki, 2002;RutkowskaKrause et al, 2003;Burbulis et al, 2005) and culture conditions (Obert et al, 2004 a).…”

Section: Introductionmentioning

confidence: 99%

Genotypic and exogenous factors affecting linseed ovary culture

Blinstrubienė

Burbulis

Masienė

2017

Zemdirbyste-Agriculture

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The current study investigated the effect of genotype, growth regulators and type of carbohydrates on callus induction and indirect shoot regeneration in ovary culture of 8 linseed (Linum usitatissimum L.) cultivars. Callogenic response varied from 9.17% to 100% depending on the cultivars and medium composition interaction. The replacement of sucrose with a combination of sucrose + maltose significantly improved the callogenesis in 3 or 4 investigated cultivars, depending on growth regulators in the induction medium. The frequency of shoot formation from ovaryderived callus in 5 responsive cultivars ranged from 4.17% to 75.00%, whereas the other three cultivars tested did not exhibit any shoots. The replacement of sucrose with a sucrose + maltose combination in induction medium reduced or completely inhibited shoot formation frequency of responsive cultivars. The significantly highest mean shoot formation frequency (52.50%) was obtained from ovary-derived callus of the cultivar ʻMikael'. The analysis of variance revealed that cultivar (C), combination of growth regulators (GR), type of carbohydrates (CH) and their interaction significantly influenced callus induction and shoot formation frequency. In most cases, a higher shoot regeneration frequency was obtained when callus was from induction medium supplemented with 2 mg l -1 thidiazuron (TDZ) + 1 mg l -1 α-naphthylacetic acid (NAA) with 6% sucrose. Cytological analysis of root tips showed that 21.88% of the regenerated plants were haploids, while another group of regenerants (78.12%) were diploid and mixoploid.

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In vitro production of haploid and doubled haploid plants from pollinated ovaries of maize (Zea mays)

Cited by 47 publications

References 9 publications

In vitro organogenesis and plant regeneration from unpollinated ovary cultures of Azadirachta indica

In vitro organogenesis and plant regeneration from unpollinated ovary cultures of Azadirachta indica

Plant Cell Culture – Present and Future

Genotypic and exogenous factors affecting linseed ovary culture

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