In-Vitro Plant Growth Promotion of Rhizobium Strains Isolated from Lentil Root Nodules under Abiotic Stresses

Sijilmassi, Badreddine; Filali-Maltouf, Abdelkarim; Fahde, Sara; En-nahli, Youness; Boughribil, Said; Kumar, Shiv; Amri, Ahmed

doi:10.3390/agronomy10071006

Cited by 9 publications

(8 citation statements)

References 61 publications

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“…For instance, while characterizing rhizobia isolated from Arachis hypogaea grown under stressed environments, Khalid et al, (2020), established the P solubilization potential of eight rhizobia through the production of organic acids. Similar results have also been reported by Harsitha et al (2020), Nagalingam et al, (2020), and Sijilmassi et al, (2020) in different plants.…”

Section: The Diversity Of P Solubilizing Rhizbacteriasupporting

confidence: 91%

Phosphate-Solubilizing Rhizobacteria: Diversity, Mechanisms, and Prospects in Sustainable Agriculture

Aloo¹,

Makumba²,

Mbega³

2020

Preprint

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Phosphorus (P) is the second-most important element after nitrogen that is required for plant growth. Although this element is abundant in most soils, it is rarely available in plant-accessible forms since most of it normally exists in soil in insoluble forms such as phosphates. In conventional agriculture, P is normally supplied as chemical fertilizer to satisfy plant P requirements. This, to a large extent, boosts plant production. However, chemical fertilizers are costly, have a huge carbon footprint, and are environmentally-unsustainable owing to the high energy requirements during their synthesis. Besides, P-containing agricultural run-offs contribute hugely to the eutrophication of water bodies and environmental degradation. Moreover, plants can consume only a small amount of chemically-supplied P since between 75 and 90% of this form of P normally get precipitated into complexes and rapidly become fixed in soil. These issues and concerns necessitate research into alternative and viable ways of supplying P to plants. Rhizobacteria have for decades been investigated in vivo and in planta as suitable tools in sustainable agriculture due to the plant-growth-promoting activities such as nutrients’ solubilization, nitrogen fixation, and production of phytohormones. Although a lot of research has been done on different nutrients-solubilizing rhizobacteria and their potential in sustainable agriculture, their mechanisms of action and prospects in sustainable agriculture remain to be fully understood. This review particularly focuses on the P solubilizing rhizobacteria and evaluates their diversity, mechanisms of action, and prospects in sustainable agriculture based on the present and future scenario of their application. Such information is useful in determining their potential and evaluating their prospects in promoting sustainable agricultural systems.

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Section: The Diversity Of P Solubilizing Rhizbacteriasupporting

confidence: 91%

Phosphate-Solubilizing Rhizobacteria: Diversity, Mechanisms, and Prospects in Sustainable Agriculture

Aloo¹,

Makumba²,

Mbega³

2020

Preprint

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“…Catalase and oxidase enzymes reduce the oxidative damage in crop while α-amylase enzyme in the aleurone layer of seed helps in hydrolyzing the endospermic starch into sugar molecules, which provide energy for vigour index parameter. [37][38][39][40][41][42][43][44] Similar results were found by Mumtaz et al 45 who reported positive results for catalase and protease activity of Bacillus strain. He also reported the cellulose degradation ability of Bacillus strains and these enzymes were comprehensively reported for their effectiveness in tolerating stress.…”

Section: Discussionsupporting

confidence: 75%

“…These results are also supported by researcher, who described that use of phosphorous solubilization and siderophore producing bacteria enhanced shoots fresh biomass, fresh root biomass, shoot length, root length, shoot dry biomass, root dry biomass as well as total dry biomass of crop. 43 Except few bacterial strains all strains had greater efficiency of producing lytic enzymes like amylase, cellulase and chitinase. Catalase and oxidase enzymes reduce the oxidative damage in crop while α-amylase enzyme in the aleurone layer of seed helps in hydrolyzing the endospermic starch into sugar molecules, which provide energy for vigour index parameter.…”

Section: Discussionmentioning

confidence: 99%

Biochemical and Molecular Evaluation of Rhizobium spp. and its Growth Promotion Studies with Lentil (Lens culinaris Medik. L.)

Banjare¹,

Patel²,

Pandey³

et al. 2023

J. Pure Appl. Microbiol.

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Growth promoting Rhizobium spp are frequently used as biofertilizers for agricultural cropping system. Furthermore, Isolation, screening and biochemical characterization of Rhizobium for a specific plant is necessary to examine ability of isolated bacteria to affect the growth and development of host plant in various ways. The current study was aimed to isolate plant specific rhizobacterial strains which are compatible with lentil (Lens culinaris Medik.L.) plant. 20 bacterial isolates have been isolated from root nodules of lentil from various agro ecological area and their biochemical characterization was performed by different plant growth promotion activities. The result showed that, among 20 isolates, four isolates have vigorous plant growth promoting activities. Four bacterial strains were able to solubilise phosphorous along with hormone production. Moreover, among four bacterial strains, two strongly produced HCN and siderophore in vitro. Subsequently, all selected bacterial isolates were inoculated in lentil seeds of variety HUL57 to study germination percentage and vigour index of the crop. Out of four isolates 26N isolate performed best growth promotion activities on lentil seedlings. Finally, on the basis of performance of bacteria on plant, four isolates were characterized using molecular approach of species identification such as 16S rRNA sequencing.

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“…As shown in Table I , the levels of IAA production by isolate 194 and USDA110 tended to reduce by more than 50% under stress conditions. Sijilmassi et al (2020) ) also showed a significant reduction in IAA production by rhizobia when grown under abiotic stress conditions such as drought at ‒2 to ‒7.5 bar and concentrated salt at 0.5 to 3% NaCl. Based on this result, other PGP traits of isolate 194 and USDA110 may also be reduced under stress conditions, and this might adversely affect or could not fully facilitate plant growth under stress conditions.…”

Section: Discussionmentioning

confidence: 94%

Enhancing the Efficiency of Soybean Inoculant for Nodulation under Multi-Environmental Stress Conditions

Wongdee

Yuttavanichakul

Longthonglang

et al. 2021

Polish Journal of Microbiology

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The development of rhizobial inoculants with increased resistance to abiotic stress is critical to mitigating the challenges related to climate change. This study aims at developing a soybean stress-tolerant Bradyrhizobium inoculant to be used under the mixed stress conditions of acidity, high temperature, and drought. Six isolates of Bradyrhizobium with high symbiotic performance on soybean were tested to determine their growth or survival abilities under in vitro conditions. The representative stress-tolerant Bradyrhizobium isolates 184, 188, and 194 were selected to test their ability to promote soybean growth under stress conditions compared to the type strain Bradyrhizobium diazoefficiens USDA110. The plant experiment indicated that isolate 194 performed better in symbiosis with soybean than other Bradyrhizobium strains under stress conditions. Based on the stress tolerance index, soybeans inoculated with isolate 194 showed a high growth performance and significantly better nodulation competition ability than USDA110 under several stress conditions. Interestingly, supplementation of sucrose in the culture medium significantly enhances the survival of the isolate and leads to improved plant biomass under various stress conditions. Analysis of the intra-cellular sugars of isolate 194 supplemented with sucrose showed the accumulation of compatible solutes, such as trehalose and glycerol, that may act as osmoprotectants. This study indicates that inoculation of stress-tolerant Bradyrhizobium together with sucrose supplementation in a medium could enhance bacterial survival and symbiosis efficiency under stress conditions. Although it can be applied for inoculant production, this strategy requires validation of its performance in field conditions before adopting this technology.

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In-Vitro Plant Growth Promotion of Rhizobium Strains Isolated from Lentil Root Nodules under Abiotic Stresses

Cited by 9 publications

References 61 publications

Phosphate-Solubilizing Rhizobacteria: Diversity, Mechanisms, and Prospects in Sustainable Agriculture

Phosphate-Solubilizing Rhizobacteria: Diversity, Mechanisms, and Prospects in Sustainable Agriculture

Biochemical and Molecular Evaluation of Rhizobium spp. and its Growth Promotion Studies with Lentil (Lens culinaris Medik. L.)

Enhancing the Efficiency of Soybean Inoculant for Nodulation under Multi-Environmental Stress Conditions

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