2000
DOI: 10.1007/s003840000242
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Abstract: We developed an in vitro model of the peritoneum by coculturing human umbilical vein endothelial cells (HUVEC) and human peritoneal mesothelial cells (HPMC) to gather information on peritoneal physiology and to closer reflect the in vivo situation in humans. HUVEC and HPMC were seeded on collagen-coated polytetraflourethylene-insert membranes of pore size 3 microm. HUVEC were grown on the bottom of the membrane and HMPC on the top. The confluent cells were monitored by measuring transepithelial resistance and … Show more

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Cited by 11 publications
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References 12 publications
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“…Continuous stimulation with TNF-a led, in concordance with previous results from our group [20], to a time-dependent increase of the IL-8 gradient and PMN transmigration. The results show that both taurolidine and polyhexanid lead dose-dependently to a significantly smaller IL-8 gradient and thus affect the PMN transmigration negatively.…”
Section: Discussionsupporting
confidence: 91%
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“…Continuous stimulation with TNF-a led, in concordance with previous results from our group [20], to a time-dependent increase of the IL-8 gradient and PMN transmigration. The results show that both taurolidine and polyhexanid lead dose-dependently to a significantly smaller IL-8 gradient and thus affect the PMN transmigration negatively.…”
Section: Discussionsupporting
confidence: 91%
“…This migration has been shown to be a reproducible mechanism in the recently developed in vitro model of human peritonitis [20]. Interleukin-8 secretion and expression of adhesion molecules were investigated as further functional parameters in our study.…”
Section: Discussionmentioning
confidence: 98%
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“…Postoperatively, an exaggerated IL-6 response precedes the clinical onset of complications by 12-48 h [39,41]. Interleukin-8 (IL-8) directs the transmigration of neutrophils [42] and has been linked to tumor development, promoting tumor cell growth in colon cancer cells [43]. IL-10 in an anti-inflammatory cytokine.…”
Section: Cytokines Studiedmentioning
confidence: 99%