In vitro ovicidal effect of a Senecio brasiliensis extract and its fractions on Haemonchus contortus

Abstract

BackgroundHaemonchosis affects sheep husbandry and its treatment is often compromised due to the development of anthelminthic resistance. Plant-derived bioactive compounds have been studied as alternative to control Haemonchus contortus. The objective of this study was to evaluate the effect of Senecio brasiliensis extracts on H. contortus egg hatching and infective larvae migration.ResultsThe aqueous extract from dried and fresh plant and alkaloid-enriched fraction of the previously dried leaves of S. brasiliensis inhibited H. contortus egg hatching. The main plant compound in alkaloid fraction was integerrimine, a pyrrolizidine alkaloid (PA). However, the aqueous extract from dried plant displayed higher efficacy when compared to their alkaloid enriched or non-polar fractions, meaning that, although PAs contributed to the ovicidal effect, other compounds in the plant can also contribute to their effect. Furthermore, the aqueous extract from dried plant also had higher efficacy than aqueous extract from fresh plant in larvae migration inhibition. Finally, extract from dried plant presented low in vitro cytotoxic effect.ConclusionTaken together our results suggest a good anthelmintic effect of S. brasiliensis, especially when aqueous extract is prepared from dried plant. Further in vivo studies should be performed focused on forms of administration of this extract in rearing sheep.

Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching

“…Thus, the ovicidal activity of AvR5 and AvR7 could be attributed to the hydroxycinnamic acid present in these fractions. Several reports assessing phenolic compounds in combined form have evidenced that they can enhance the anthelmintic effect ( Klongsiriwet et al, 2015 ; von Son-de Fernex et al, 2015 ; Mendonça Soares et al, 2019 ). Chlorogenic acid and chatequin have been reported in A. oxycedri , another mistletoe specie ( Orhan et al ., 2019 ).…”

Effects of Senecio selloi (Spreng.) DC flower extract on mitochondrial membrane peroxidation and enzyme activities

“…S. chrysanthemoides DC possess Pas [37] , such as integerrimine, senecionine and retrorsine, among others [38] . According to Mendoça Soares et al (2019), S. brasiliensis contains the pyrrolizidine alkaloid integerrimine [39] . For Senecio scandens Buch.-Ham.…”