In vitro Osteoinduction Potential of a novel Silica Coated Hydroxyapatite Bioscaffold Seeded with Rabbit Mesenchymal Stem Cell

Ravindran, Ninu Ajantha; Maiti, Swapan Kumar; Palakkara, Sangeetha; Kritaniya, Deepika; Mahan, Tamil; Kumar, Naveen

doi:10.15406/jsrt.2016.01.00009

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…Although calcium levels reportedly rise sharply around 15–21 days post-surgery, our study observed an increase in phosphorus levels on the 30th day. Overall, increased phosphorus levels were noted in all groups except the control, consistent with previous findings [ 28 ]. Ionized calcium levels decreased during fracture healing, likely due to long-term BMP-2 administration favoring higher bone formation.…”

Section: Discussionsupporting

confidence: 91%

“…Ionized calcium levels decreased during fracture healing, likely due to long-term BMP-2 administration favoring higher bone formation. BMP-2 lowers calcium non-significantly in a 60-day bone healing study [ 28 ]. Ionized calcium, crucial for bone formation, decreases during progressive phases of bone tissue regeneration [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

“…Animals in group F received both three million rBMSC (P3) at the scaffold implantation site on days 7, 14, and 21 and 0.4 µg/mL BMP-2 on days 30, 45, and 60. At 90 days post-implantation, the animals were euthanized following standard guidelines [ 27 ], and the experimental bone was harvested for further investigations [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

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Bone Marrow-Derived Mesenchymal Stem Cell-Laden Nanocomposite Scaffolds Enhance Bone Regeneration in Rabbit Critical-Size Segmental Bone Defect Model

Kalaiselvan,

Maiti,

Shivaramu

et al. 2024

JFB

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Bone regeneration poses a significant challenge in the field of tissue engineering, prompting ongoing research to explore innovative strategies for effective bone healing. The integration of stem cells and nanomaterial scaffolds has emerged as a promising approach, offering the potential to enhance regenerative outcomes. This study focuses on the application of a stem cell-laden nanomaterial scaffold designed for bone regeneration in rabbits. The in vivo study was conducted on thirty-six healthy skeletally mature New Zealand white rabbits that were randomly allocated into six groups. Group A was considered the control, wherein a 15 mm critical-sized defect was created and left as such without any treatment. In group B, this defect was filled with a polycaprolactone–hydroxyapatite (PCL + HAP) scaffold, whereas in group C, a PCL + HAP-carboxylated multiwalled carbon nanotube (PCL + HAP + MWCNT-COOH) scaffold was used. In group D, a PCL + HAP + MWCNT-COOH scaffold was used with local injection of bone morphogenetic protein-2 (BMP-2) on postoperative days 30, 45, and 60. The rabbit bone marrow-derived mesenchymal stem cells (rBMSCs) were seeded onto the PCL + HAP + MWCNT-COOH scaffold by the centrifugal method. In group E, an rBMSC-seeded PCL + HAP + MWCNT-COOH scaffold was used along with the local injection of rBMSC on postoperative days 7, 14, and 21. For group F, in addition to the treatment given to group E, BMP-2 was administered locally on postoperative days 30, 45, and 60. Gross observations, radiological observation, scanning electron microscopic assessment, and histological evaluation study showed that group F displayed the best healing properties, followed by group E, group D, group C, and B. Group A showed no healing with ends blunting minimal fibrous tissue. Incorporating growth factor BMP-2 in tissue-engineered rBMSC-loaded nanocomposite PCL + HAP + MWCNT-COOH construct can augment the osteoinductive and osteoconductive properties, thereby enhancing the healing in a critical-sized bone defect. This novel stem cell composite could prove worthy in the treatment of non-union and delayed union fractures in the near future.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Bone Marrow-Derived Mesenchymal Stem Cell-Laden Nanocomposite Scaffolds Enhance Bone Regeneration in Rabbit Critical-Size Segmental Bone Defect Model

Kalaiselvan,

Maiti,

Shivaramu

et al. 2024

JFB

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Culture, Characterization and Differentiation Potential of Rat Bone Marrow derived Mesenchymal Stem Cells

Tamilmahan¹

2016

JSRT

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Abbreviations: rBM-MSC, rat bone marrow derived mesenchymal stem cells; CFU-F, colony forming unit-fibroblast; DPBS, dulbecco's phosphate buffer saline; DMEM, dulbecco's modified eagles medium; RT PCR, reverse transcription polymerase chain reaction; RNA, ribonucleic acid IntroductionBone marrow hosts variety of tissues including hematopoietic lineage cells and mesenchymal stem cells (MSCs). The hematopoietic cells are the major source of the blood cells in the adult body which are regulated within the microenvironment of the stromal cells of the bone marrow.1,2 MSCs are multipotent cells and can be differentiate into various cell lineage depend upon their environment and culture condition in which they are kept. Rat bone marrow derived stem cells (rBM-MSCs) have long term self renewing and capabilities of pluripotency including osteoblast, adipocytes, chondrocytes, tenocytes, muscle cells and neurogenic cells, which make them ideal source of stem cells for regeneration of injured tissue. [3][4][5] Bone marrow derived mesenchymal stem cells have been isolated from different species. Report says that each species having different culturing and growing capacity. 6 For example human bone marrow derived stem cells are easy to harvest and maintain in culture condition where as rat MSCs is more difficult compared to other species. 7,8Technical difficulties in isolation limited the number of animal experiment and its required animal transplantation model for pre clinical studies.9 Density gradient centrifugation, 10 plastic adherence 11 and immunomagnetic selection 12,13 like various methods are available for isolation of MSCs from bone marrow. No appropriate methods are available for selection of suitable cells. Each method has their own pros and cons. Therefore this study combined density gradient centrifugation and plastic adherence for easy and reliable method for selection of suitable cells. Materials and methods Collection of bone marrowFour male wistar rats aged 8-14weeks old were prepared for collection of bone marrow. All procedure was undertaken after getting prior approval from Institutional Animal Ethics Committee (IAEC). Briefly after euthanasia, animals back side were cleanly shaved and prepared aseptically. The skin incision was made on the lateral aspect of thigh and both femur and tibia from one rat were taken after stripping out adherent muscles of the knee end. The collected bone was taken to laminar hood and placed in Petri dish containing DMEM (Dulbecco's Modified Eagles Medium) media with antibiotics. Both the metaphyseal region of femur was cut with scissors and needle was inserted into the bone to aspirate the content by flushing with 1ml of complete media. The cell suspension was centrifuged at 980rpm for 5min to concentrate the cells. The cell pellets were resuspended with 5ml of complete DMEM medium then layered over HISTOPAQUE -1077(Sigma) and centrifuged at 2500rpm for 30minutes. Mononuclear cells were collected from the interface by gradient centrifugation and washed with Ca+ and Mg+ free ...

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In vitro CYTOTOXIC EFFECTS OF HYBRID GRAPHENE OXIDE NANOCOMPOSITES (hGONCs) ON CRYOPRESERVED CAPRINE WHARTON’S JELLY DERIVED MESENCHYMAL STEM CELLS (WJ-MSCs)

Dhenge¹,

Gade²,

Op³

et al. 2019

JEBAS

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In present study, caprine Wharton's jelly derived mesenchymal stem cells (WJ-MSCs) were isolated and characterized by osteogenic and adipogenic differentiation. Total 12 hybrid graphene oxide nanocomposites (hGO NCs) such as N2 doped GO-HA NCs, P doped GO-HA NCs, S doped GO-HA NCs, N2 doped GO-SiO2 NCs, P doped GO-SiO2 NCs, S doped GO-SiO2 NCs, N2 doped GO-TiO2 NCs, P doped GO-TiO2 NCs, S doped GO-TiO2 NCs, N2 doped GO-Au NCs, P doped GO-Au NCs and S doped GO-Au NCs were incorporated at doses 100, 50, 25 , 10 and 0 µg/ml in vitrification solution. Caprine WJ-MSCs were cryopreserved by using open pulled straw (OPS) vitrification method and analysed hGO NCs effect on their post thaw viability and culture characteristics. Caprine WJ-MSCs were exhibited normal fibroblastoid morphology and differentiated in to osteogenic and adipogenic lineages. The significant (P<0.01) highest and lowest caprine WJ-MSCs post thaw viability (cumulatively) was observed in P-GO-TiO2 NC and P-GO-HA NC groups, respectively along with all doses (cumulatively) significantly (P<0.01) decreased post thaw cell viability as compared with control. All hGO NCs were significantly (P<0.01) decreased caprine WJ-MSCs post thaw viability at doses 100 and 50 µg/ml as compared to 25, 10 and 0 µg/ml doses. Post thaw caprine WJ-MSCs were grew in normal pattern with similar fibroblast like morphology on days 14. In conclusion, all hGO NCs at doses 50 and 100 µl/ml are cytotoxic and P-GO-TiO2 NC is least decrease caprine WJ-MSCs post thaw viability.

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In vitro Osteoinduction Potential of a novel Silica Coated Hydroxyapatite Bioscaffold Seeded with Rabbit Mesenchymal Stem Cell

Cited by 3 publications

References 29 publications

Bone Marrow-Derived Mesenchymal Stem Cell-Laden Nanocomposite Scaffolds Enhance Bone Regeneration in Rabbit Critical-Size Segmental Bone Defect Model

Bone Marrow-Derived Mesenchymal Stem Cell-Laden Nanocomposite Scaffolds Enhance Bone Regeneration in Rabbit Critical-Size Segmental Bone Defect Model

Culture, Characterization and Differentiation Potential of Rat Bone Marrow derived Mesenchymal Stem Cells

In vitro CYTOTOXIC EFFECTS OF HYBRID GRAPHENE OXIDE NANOCOMPOSITES (hGONCs) ON CRYOPRESERVED CAPRINE WHARTON’S JELLY DERIVED MESENCHYMAL STEM CELLS (WJ-MSCs)

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