In vitro new dialysis protocol to assay the antiseptic properties of a quaternary ammonium compound polymerized with denture acrylic resin

Abstract

Aims: To develop an in vitro protocol in order to assess the antiseptic properties of a quaternary ammonium compound polymerized with acrylic denture resin base, using experimental resin discs and dialysis membranes. Methods and Results: Experimental acrylic resin discs were polymerized with Poly 202063A, an ammonium compound (2-50%). Antiseptic properties were assayed against two reference strains (Escherichia coli, Staphylococcus aureus) and a laboratory strain (Candida albicans), using three different conditions (test A, B and C). In test A, according to classical protocols the resin discs were first soaked in large volumes of microbial inoculum (45 ml). An original dialysis protocol was then designed to recreate the small biofilm volume on the prosthetic surface. In test B, discs and bacterial inoculum (600 ll) were introduced in a dialysis bag and dialysed against a sterile buffer. A bactericidal effect was observed against E. coli and Staph. aureus (<0AE1% viable cells in initial bacterial suspension). A dose-dependent fungistatic effect was observed against C. albicans. Finally, in test C discs and sterile buffer (600 ll) were introduced in a dialysis bag and dialysed against microbial inoculum. Reduced activity was found outside the dialysis bag, demonstrating that free ammonium was able to diffuse through the dialysis membrane, displaying antiseptic properties.Conclusions: The present protocol demonstrated that a quaternary ammonium compound remains efficient after heat polymerization with an acrylic denture base resin, both in immediate and distant microbial environments. Significance and Impact of the Study: Such removable prosthetic devices with intrinsic antiseptic properties would contribute to improve the long-term management of denture stomatitis.

Antifungal Effect of Acrylic Resin Containing Apatite-coated TiO2 Photocatalyst

“…The first one pertained to the degree of conversion of acrylic resin. Some studies reported that the Fs value decreased with increase in the content of other compounds added to acrylic resin 30,39) . In the context of the present study, the degree of conversion of acrylic resin might be adversely affected because of the addition of other material namely TiO2 or Ap-TiO2.…”

“…Against this backdrop of functional and costrelated reservations toward denture cleansers, several researchers have attempted to mix acrylic resins with antifungal agents, antiseptics, a silver inorganic system, and organic materials for additional antibacterial effects [29][30][31][32] . These previous studies showed that the added antifungal and antiseptic agents were rapidly released from denture bases.…”

Quaternary ammonium compounds in dental restorative materials

“…Accordingly, Pesci-Bardon et al 61,62) have performed a study that a quaternary ammonium compound was incorporated into an acrylic denture resin base in different concentrations, and the antimicrobial properties to Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa), and Candida albicans (C. albicans) were tested. It was shown that 2% quaternary ammonium compound polymerized with a denture acrylic resin displayed antimicrobial properties after a 4-week soaking period in artificial saliva.…”

Incorporação de ácido metacrílico em resinas acrílicas para base de prótese

“…Além disso, a menor destreza manual de pacientes idosos prejudica a higiene bucal e predispõe a instalação da estomatite protética (BUDTZ-JORGENSEN, 1978). Essa condição geralmente não é considerada séria, entretanto pode resultar num processo inflamatório crônico e agir como um foco de infecção (BUDTZ-JORGENSEN, 1978), especialmente em pacientes que apresentam alguns fatores sistêmicos como diabetes mellitus, AIDS, doenças neoplásicas, quimioterapia, radioterapia e terapia antibiótica de largo espectro (PESCI-BARDON et al, 2004).…”

“…Diversos materiais têm sido testados com intuito de diminuir a aderência de microorganismos à resina acrílica como a adição de clorexidina (LAMB & MARTIN, 1983), compostos quaternários de amônio (PESCI-BARDON et al, 2004), radicais fosfato (PURI et al, 2008), zeólito de prata-zinco (CASEMIRO et al, 2008) e outros.…”