In Vitro Models of Chronic Obstructive Pulmonary Disease (COPD)

Adamson, Jason; Haswell, Linsey E.; Phillips, Gary; Gaça, Marianna

doi:10.5772/18247

Cited by 30 publications

(30 citation statements)

References 105 publications

(88 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Traditionally, biological testing of tobacco smoke constituents has been more routine, involving exposure to particulate matter extracted in a solvent or buffer, and applied to cells under a submerged condition [32]. Although these submerged lung culture assessments are quick and comparatively cheap (compared to whole smoke exposure systems), they do not reflect the physiological condition of the lung organ and its epithelial cells which sit at the blood-gas barrier and are exposed to air and other inhalable substances at the ALI.…”

Section: Discussionmentioning

confidence: 99%

Assessment of cigarette smoke particle deposition within the Vitrocell® exposure module using quartz crystal microbalances

Adamson

Thorne

Dalrymple

et al. 2013

Chemistry Central Journal

Self Cite

View full text Add to dashboard Cite

BackgroundCigarette smoking is a cause of a variety of serious diseases, and to understand the toxicological impact of tobacco smoke in vitro, whole smoke exposure systems can be used. One of the main challenges of the different whole smoke exposure systems that are commercially available is that they dilute and deliver smoke in different ways, limiting/restricting the cross-comparison of biological responses. This is where dosimetry – dose quantification – can play a key role in data comparison. Quartz crystal microbalance (QCM) technology has been put forward as one such tool to quantify smoke particle deposition in vitro, in real-time.ResultsUsing four identical QCMs, installed into the Vitrocell® mammalian 6/4 CF Stainless exposure module, we were able to quantify deposited smoke particle deposition, generated and diluted by a Vitrocell® VC 10 Smoking Robot. At diluting airflows 0.5-4.0 L/min and vacuum flow rate 5 ml/min/well through the exposure module, mean particle deposition was in the range 8.65 ± 1.51 μg/cm2-0.72 ± 0.13 μg/cm2. Additionally, the effect of varying vacuum flow rate on particle deposition was assessed from 5 ml/min/well - 100 ml/min/well. Mean deposited mass for all four airflows tested per vacuum decreased as vacuum rate was increased: mean deposition was 3.79, 2.75, 1.56 and 1.09 μg/cm2 at vacuum rates of 5, 10, 50 and 100 ml/min/well respectively.ConclusionsQCMs within the Vitrocell® exposure module have demonstrated applicability at defining particle dose ranges at various experimental conditions. This tool will prove useful for users of the Vitrocell® system for dose–response determination and QC purposes.

show abstract

Section: Discussionmentioning

confidence: 99%

Assessment of cigarette smoke particle deposition within the Vitrocell® exposure module using quartz crystal microbalances

Adamson

Thorne

Dalrymple

et al. 2013

Chemistry Central Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…CS has been shown to either activate or inhibit activation of cells in culture (Adamson et al 2011). We have shown that smoke increases expression of CD14, HLA-DR, CD11a, and CD11b antigens on THP1 cells.…”

Section: Discussionmentioning

confidence: 85%

“…Moreover, a number of compounds found in CS can directly damage lung tissue and induce oxidative imbalance, adding noxious exogenous chemical stimuli to complex endogenous inflammation. The molecular mechanism responsible for COPD is unknown and several animal and cellular models were described to mimic human disease and to study different aspects of the disease (Adamson et al 2011). Cell lines are commonly used in in vitro studies to model COPD.…”

Section: Introductionmentioning

confidence: 99%

Crosstalk Between Co-cultured A549 Cells and THP1 Cells Exposed to Cigarette Smoke

Hołownia

Wielgat

Kwołek

et al. 2015

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Cigarette smoke (CS) is considered as a major etiological factor in the pathogenesis of chronic obstructive pulmonary disease. In this study we used A549 cells and THP-1 cells grown for 24 h in monoculture or in co-culture in CS-conditioned media and changes in their proliferation, viability, acetylated histone H3 levels and expression of extracellular antigens CD14, HLA-DR, CD11a, and CD11b were assessed. CS was highly toxic to A549 cells but not to THP1 cells. In A549 cells, oxidative stress reached the highest values after 1 h of CS exposure and then decreased. In THP1 cells oxidative stress was lower and increased progressively with time. CS decreased proliferation of A549 and THP1 cells by about 80% and 21%, respectively. CS did not alter acetylated histone H3 levels in A549 cells, while in THP1 cells the levels were reduced by about 35%. CS significantly increased expression of CD14, HLA-DR, CD11a, and CD11b in THP1 cells. In co-culture, naïve or CS-pretreated THP1 cells significantly protected A549 cells against CS toxicity but had higher death rates. These results show that epithelial cells are more fragile to CS than monocytes and that CS-activated monocytes may protect epithelial cells against CS-induced cytotoxicity.

show abstract

“…There is no known cure for these diseases but the symptoms are treatable and their progression can be delayed. Inhaling irritants lead to an accumulation of inflammatory cells including neutrophils, CD8 + T-Lymphocytes, B cells and macrophages [120][121][122], well-illustrated in the review by Vlahos and Bozinovski [114]. Adamson et al summarized cell culture models suitable to mimic the situation in COPD [119], whereas Huang et al discussed different cell lines mimicking asthma [123].…”

Section: Pneumoniamentioning

confidence: 98%

Preclinical safety and efficacy models for pulmonary drug delivery of antimicrobials with focus on in vitro models

Hittinger

Juntke

Kletting

et al. 2015

Advanced Drug Delivery Reviews

View full text Add to dashboard Cite

In Vitro Models of Chronic Obstructive Pulmonary Disease (COPD)

Cited by 30 publications

References 105 publications

Assessment of cigarette smoke particle deposition within the Vitrocell® exposure module using quartz crystal microbalances

Assessment of cigarette smoke particle deposition within the Vitrocell® exposure module using quartz crystal microbalances

Crosstalk Between Co-cultured A549 Cells and THP1 Cells Exposed to Cigarette Smoke

Preclinical safety and efficacy models for pulmonary drug delivery of antimicrobials with focus on in vitro models

Contact Info

Product

Resources

About