In vitro modeling of endometriosis and endometriotic microenvironment – Challenges and recent advances

Gołąbek-Grenda, Agata; Olejnik, Anna

doi:10.1016/j.cellsig.2022.110375

Cited by 14 publications

(12 citation statements)

References 158 publications

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“…The present study determined the cytotoxic effect of the analyzed stilbenes on human endometrial stromal cells (T HESC) and human endometriotic epithelial cells (12Z). The 12Z cell line retains characteristics of the active phase of endometriosis and is considered suitable for studying the cellular and molecular context of endometriosis 19 . In contrast, the T HESC line represents a distinct stromal compartment of the endometrium and resembles typical endometrial features; therefore, it has been widely applied in endometriosis studies 20 , 21 .…”

Section: Discussionmentioning

confidence: 99%

Natural resveratrol analogs differentially target endometriotic cells into apoptosis pathways

Gołąbek-Grenda

Kaczmarek

Juzwa

et al. 2023

Sci Rep

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The specific characteristics of endometriotic cells are their ability to evade the apoptotic machinery and abnormal response to apoptotic stimuli. Natural-originated compounds may constitute a beneficial strategy in apoptosis modulation in endometriosis. We investigated and compared the potency of natural resveratrol analogs, including piceatannol, polydatin, and pterostilbene, in targeting cell death pathways, including apoptosis-related morphologic and biochemical processes, alongside the modulation of the critical genes expression. Upon resveratrol and pterostilbene treatment, a significant reduction of endometriotic cell viability and an increased apoptotic proportion of cells were noted. The lower antiproliferative potential was found for piceatannol and polydatin. Endometrial stromal T HESC cells were significantly more resistant than endometriotic epithelial 12Z cells to the cytotoxic activity of all analyzed compounds. They differentially affected endometriotic cell viability, cell cycle, anti- and proapoptotic genes regulation, caspases expression and enzymatic activity, and DNA fragmentation. Pterostilbene-mediated endometriotic cell apoptosis modulation was confirmed to be most effective but without evident caspase 3 upregulation. Our study provides valuable insight into the apoptogenic activity of resveratrol and its natural analogs in endometriotic cells. Data obtained revealed the highest therapeutic potential of pterostilbene by effectively targeting cell death determinants in endometriosis, strengthening its optimization in further extensive research.

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Section: Discussionmentioning

confidence: 99%

Natural resveratrol analogs differentially target endometriotic cells into apoptosis pathways

Gołąbek-Grenda

Kaczmarek

Juzwa

et al. 2023

Sci Rep

Self Cite

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“…Endometriosis refers to the ectopic growth of the endometrium in the peritoneal cavity or ovaries, which is illustrated by Sampson's retrograde menstruation theory, closely related to aberrant responses of the endometrial tissue to sexual hormones [ 111 ]. In vitro models based on bioengineering have great advantages according to the particularity of spontaneous menstruation, compared to animal models or cell culture systems [ 112 ].…”

Section: Application Of Bioengineering Technology In Endometrial Dise...mentioning

confidence: 99%

“…In contrast, the application of microfluidics focuses on mechanical alterations, which govern junctional adhesion and cytoskeletal rearrangement in cellular activities and play an indispensable role in affecting the migration of endometrial stromal cells [ 44 ]. Thus, the combined use of endometrial organoids and microfluidic chips has great potential for developing models for endometriosis and embryo implantation [ 49 , 112 , 210 ]. Therefore, endometrial organoids and co-culture systems with bioengineered scaffolds are expected to be used for studying specific niches of the functional and basal layers, thus deepening our understanding of the endometrium.…”

Section: Challenges and Future Perspectivesmentioning

confidence: 99%

Bioengineering approaches for the endometrial research and application

Dai,

Liang,

Guo

et al. 2024

Materials Today Bio

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“…These systems enable the precise manipulation of the cellular microenvironment, allowing for the co-culture of various cell types, such as endometrial stromal cells, immune cells, and fibromuscular cells, to more accurately reflect the complexities of the diseased tissue. Microfluidic fabrication of in vitro endometrial models can elucidate crucial cellular and molecular determinants in pathologies and screen pharmacological agents, facilitating the acquisition of research materials, and reducing ethical dilemmas and expenses [ 241 ]. By recapitulating the interactions between endometrial stromal cells and peritoneal mesothelial cells in patients with endometriosis, these models have shed light on the role of peritoneal physiology in the development of endometriosis [ 242 ], which found that endometriosis can invade endometrial stromal cell and separate peritoneal mesothelial cells, indicating that peritoneal physiology has an important impact on the development of endometriosis.…”

Section: Microfluidic Chips For Gynecological Diseasesmentioning

confidence: 99%

Revolutionizing the female reproductive system research using microfluidic chip platform

Yan,

Wu,

Zhang

et al. 2023

J Nanobiotechnol

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Comprehensively understanding the female reproductive system is crucial for safeguarding fertility and preventing diseases concerning women's health. With the capacity to simulate the intricate physio- and patho-conditions, and provide diagnostic platforms, microfluidic chips have fundamentally transformed the knowledge and management of female reproductive health, which will ultimately promote the development of more effective assisted reproductive technologies, treatments, and drug screening approaches. This review elucidates diverse microfluidic systems in mimicking the ovary, fallopian tube, uterus, placenta and cervix, and we delve into the culture of follicles and oocytes, gametes’ manipulation, cryopreservation, and permeability especially. We investigate the role of microfluidics in endometriosis and hysteromyoma, and explore their applications in ovarian cancer, endometrial cancer and cervical cancer. At last, the current status of assisted reproductive technology and integrated microfluidic devices are introduced briefly. Through delineating the multifarious advantages and challenges of the microfluidic technology, we chart a definitive course for future research in the woman health field. As the microfluidic technology continues to evolve and advance, it holds great promise for revolutionizing the diagnosis and treatment of female reproductive health issues, thus propelling us into a future where we can ultimately optimize the overall wellbeing and health of women everywhere. Graphical Abstract

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In vitro modeling of endometriosis and endometriotic microenvironment – Challenges and recent advances

Cited by 14 publications

References 158 publications

Natural resveratrol analogs differentially target endometriotic cells into apoptosis pathways

Natural resveratrol analogs differentially target endometriotic cells into apoptosis pathways

Bioengineering approaches for the endometrial research and application

Revolutionizing the female reproductive system research using microfluidic chip platform

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