In vitro method to evaluate the barrier properties of medical devices for cutaneous use

Casiraghi, Antonella; Ranzini, Francesco; Musazzi, Umberto M.; Franzè, Silvia; Méloni, Marisa; Minghetti, Paola

doi:10.1016/j.yrtph.2017.08.007

Cited by 14 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The stirring rate and temperature of receptor solution were, respectively, kept at 600 rpm and 37°C. At appropriate intervals (15 min, 30 min, 45 min, 1 h, 2 h, 4 h, 6 h and 8 h) 600 μL aliquots of the receptor medium were withdrawn with a syringe and immediately replaced with equal volumes of fresh receptor phase (Casiraghi et al, 2017). The cumulative quantity of compounds was determined by HPLC-DAD-ESI/MS.…”

Section: Ex Vivo Skin Permeation Studiesmentioning

confidence: 99%

Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies

Taofiq

Rodrigues

Barros

et al. 2019

Food and Chemical Toxicology

View full text Add to dashboard Cite

Mushrooms are important sources of natural bioactive compounds that are increasingly used as cosmeceutical ingredients. In this context, ethanolic extracts were prepared from Ganoderma lucidum and Pleurotus ostreatus and tested by incorporation into base cosmetic creams. In vitro safety evaluation of the extracts and cosmetic formulations prepared therefore was carried out using the MTT and LDH assays in keratinocyte (HaCaT) and fibroblast (HFF-1) cell lines. Moreover, they were submitted to ex vivo skin permeation studies using a Franz diffusion apparatus with pig ear skin as permeation membrane. The results showed the absence of toxicity for keratinocytes and fibroblasts in a concentration dependent manner, which is indicative of the safety of these extracts for cosmeceutical ingredients purposes. Protocatechuic and syringic acids were the only compounds permeating from G. lucidum extract in the first 8 h of study, no penetration was observed for phenolic acids found in P. ostreatus extract and formulation. These results pointed out for the suitability of using mushroom extracts as skin care ingredients and may contribute for the valorisation of wastes generated by the mushroom processing industry, which can serve as raw-materials for the obtainment of the extracts.

show abstract

Section: Ex Vivo Skin Permeation Studiesmentioning

confidence: 99%

Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies

Taofiq

Rodrigues

Barros

et al. 2019

Food and Chemical Toxicology

View full text Add to dashboard Cite

show abstract

“… 20 , 21 The reduction in caffeine passage after application of the product was used as an index of the efficacy to reduce transepithelial passage. 22 Similar experimental approaches have been applied to different mucosae, such as oral reconstructed epithelium, showing that caffeine penetration kinetics are completed within 2 h. 23 In the present study, the application of a relatively high volume of caffeine solution (100 μL) at pH 3.3 to the oesophageal epithelium allowed us to better mimic the acidic environment of the oesophagus of GORD patients.…”

Section: Discussionmentioning

confidence: 62%

Protective Mechanisms of Liquid Formulations for Gastro-Oesophageal Reflux Disease in a Human Reconstructed Oesophageal Epithelium Model

Ceriotti¹,

Buratti²,

Corazziari³

et al. 2022

MDER

Self Cite

View full text Add to dashboard Cite

Purpose A novel experimental design based on a human-reconstructed oesophageal epithelium (HO2E) model has been applied to quantitively assess the properties of a set of liquid formulations, Device A (Gerdoff ® Protection), Device B (Esoxx ® One), and Device C (Marial ® gel) developed to form a temporary physical barrier on the oesophageal epithelium and modify epithelial permeability so to protect the oesophageal mucosa from refluxate components. Methods The formulations were applied to a prewetted HO2E model for 15 min. Then, a 0.5% caffeine solution was applied, and its penetration kinetics was assessed at 1 h and 2 h in acidic environments (pH= 3.3) to mirror exposure of the oesophageal mucosa to acidic reflux in GORD patients. Caffeine permeated into the basolateral compartment (evaluated by HPLC-UV) and Lucifer yellow (LY) permeability were quantified 15 min after application of the caffeine in acidic environments. Results At the 15 min timepoint, Device A reduced caffeine permeation by 77.2% and LY flux by 30.4% compared to the untreated control and with a faster mode of action than that of the other liquid formulations. Transepithelial caffeine flux was reduced, albeit with different timing and efficiency, by all three compounds up to the end of the 2 hour experiment. At 1 h, Device A reduced the caffeine flux by 79.2%; Device B, by 67.2%; and Device C, by 37%. Conclusion These results confirm the ability of the medical devices tested to interact with the oesophageal epithelium and create a temporary physical protective film for up to 2 hours after their application. The results underline differences in the mechanism of action of the three medical devices, with Device A performing faster than the other formulations. The overall results support the relevance of the reconstructed mucosal model to investigate oesophageal epithelium–product interactions and precisely differentiate liquid formulation performance.

show abstract

“…This compound has an occlusive effect. It can be used in a standardized test procedure as a standard reference substance in evaluation of protective or barrier cream against water-soluble and water-insoluble irritants [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Thiolated Silicone Oils as New Components of Protective Creams in the Prevention of Skin Diseases

et al. 2021

View full text Add to dashboard Cite

This work investigates the possibility of using thiolated silicone oils as new components in protective creams and their impact on the efficacy of these products. Thiolated silicone oils were synthesized by amide bond formation between primary amino groups of poly17dimethylsiloxane-co-(3-aminopropyl)-methylsiloxane] and carboxylic groups of thiol ligand (3-mercaptopropionic acid) with carbodiimide as a coupling agent. To evaluate and compare the properties of these kinds of thiomers, three different emulsion o/w types were obtained. Emulsion E1 contained methyl silicone oil, E2 poly[dimethylsiloxane-co-(3-aminopropyl)-methylsiloxane], and E3 thiolated silicone oil (silicone-MPA), respectively. Physicochemical properties, including pH, conductivity, droplet size distribution, viscosity, and stability, were assessed. The efficacy of barrier creams in the prevention of occupational skin diseases depends on their mechanical and rheological properties. Thus, the method which imitates the spreadability conditions on the skin and how structure reconstruction takes places was performed. We also investigated textural profile, bioadhesion, protection against water and detergents, and water vapor permeability. Emulsion E3 was characterized by beneficial occlusion, spreadability, and adhesion properties. These features with prolonged residence time on the skin can make designed barrier creams more preferable for consumers.

show abstract

In vitro method to evaluate the barrier properties of medical devices for cutaneous use

Cited by 14 publications

References 26 publications

Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies

Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies

Protective Mechanisms of Liquid Formulations for Gastro-Oesophageal Reflux Disease in a Human Reconstructed Oesophageal Epithelium Model

Thiolated Silicone Oils as New Components of Protective Creams in the Prevention of Skin Diseases

Contact Info

Product

Resources

About