In Vitro Melanoma Cell Growth After Preenucleation Radiation Therapy

Extrascleral Tumor Extension Associated with Localized Scleral Melt following Plaque Brachytherapy for Uveal Melanoma: Clinical and Histologic Findings

“…In studies in which histopathologic analyses were performed on post-enucleation specimens without scleral melt, less mitotic activity was found in post-plaque-brachytherapy eyes compared to those primarily enucleated, even though the authors note that whether this represents a direct cytotoxicity/radiation effect or slower-growing tumors at baseline is difficult to determine [12,13,14]. Histologic examinations and in vitro studies of melanoma cell growth after pre-enucleation external beam radiation have found decreased mitotic and Ki-67 activity, and decreased cell adhesion, growth, and viability in cell culture, attributable to the radiation effect [15,16,17]. Meanwhile, another study found no mitotic figures and furthermore found evidence of “reproductive cell death” (defined as increased doubling time) in 3 fresh post-irradiation samples submitted for cell culture [18] (though it is worth noting that none of the samples submitted for cell culture were from eyes enucleated for suspicion of tumor regrowth).…”

Extrascleral Tumor Extension Associated with Localized Scleral Melt following Plaque Brachytherapy for Uveal Melanoma: Clinical and Histologic Findings

“…In studies in which histopathologic analyses were performed on post-enucleation specimens without scleral melt, less mitotic activity was found in post-plaque-brachytherapy eyes compared to those primarily enucleated, even though the authors note that whether this represents a direct cytotoxicity/radiation effect or slower-growing tumors at baseline is difficult to determine [12,13,14]. Histologic examinations and in vitro studies of melanoma cell growth after pre-enucleation external beam radiation have found decreased mitotic and Ki-67 activity, and decreased cell adhesion, growth, and viability in cell culture, attributable to the radiation effect [15,16,17]. Meanwhile, another study found no mitotic figures and furthermore found evidence of “reproductive cell death” (defined as increased doubling time) in 3 fresh post-irradiation samples submitted for cell culture [18] (though it is worth noting that none of the samples submitted for cell culture were from eyes enucleated for suspicion of tumor regrowth).…”

Uveal melanoma: therapeutic consequences of doubling times and irradiation results; a review

“…Studies on pre-operatively irradiated human melanoma cells in tissue culture substantiate that irradiation indeed decreases the attachment, growth and viability of melanoma cells in these cultures, while microscopic examination indicated a reduced mitotic activity [7,16,37,63]. No beneficial effect of pre-enucleation irradiation could, however, be observed in terms of better survival after pre-enucleation irradiation as compared with control groups [9,15,40].…”

Effect of Pre-Enucleation Irradiation on the Survival of Patients with Uveal Melanoma

“…Studies in mice show that the probability of developing metastasis after pre-enucleation radiotherapy is low [7]. Although pre-enucleation radiotherapy seems to decrease the degree of mitotic activity in melanoma cells and to inhibit in vitro growth of tumor cells [8], available data suggest that it does not improve survival.…”

Extrascleral Tumor Extension Associated with Localized Scleral Melt following Plaque Brachytherapy for Uveal Melanoma: Clinical and Histologic Findings

“…In studies in which histopathologic analyses were performed on post-enucleation specimens without scleral melt, less mitotic activity was found in post-plaque-brachytherapy eyes compared to those primarily enucleated, even though the authors note that whether this represents a direct cytotoxicity/radiation effect or slower-growing tumors at baseline is difficult to determine [12,13,14]. Histologic examinations and in vitro studies of melanoma cell growth after pre-enucleation external beam radiation have found decreased mitotic and Ki-67 activity, and decreased cell adhesion, growth, and viability in cell culture, attributable to the radiation effect [15,16,17]. Meanwhile, another study found no mitotic figures and furthermore found evidence of “reproductive cell death” (defined as increased doubling time) in 3 fresh post-irradiation samples submitted for cell culture [18] (though it is worth noting that none of the samples submitted for cell culture were from eyes enucleated for suspicion of tumor regrowth).…”

Uveal melanoma: therapeutic consequences of doubling times and irradiation results; a review

“…Studies on pre-operatively irradiated human melanoma cells in tissue culture substantiate that irradiation indeed decreases the attachment, growth and viability of melanoma cells in these cultures, while microscopic examination indicated a reduced mitotic activity [7,16,37,63]. No beneficial effect of pre-enucleation irradiation could, however, be observed in terms of better survival after pre-enucleation irradiation as compared with control groups [9,15,40].…”

Effect of Pre-Enucleation Irradiation on the Survival of Patients with Uveal Melanoma

“…Studies in mice show that the probability of developing metastasis after pre-enucleation radiotherapy is low [7]. Although pre-enucleation radiotherapy seems to decrease the degree of mitotic activity in melanoma cells and to inhibit in vitro growth of tumor cells [8], available data suggest that it does not improve survival.…”

Extrascleral Tumor Extension Associated with Localized Scleral Melt following Plaque Brachytherapy for Uveal Melanoma: Clinical and Histologic Findings

“…In studies in which histopathologic analyses were performed on post-enucleation specimens without scleral melt, less mitotic activity was found in post-plaque-brachytherapy eyes compared to those primarily enucleated, even though the authors note that whether this represents a direct cytotoxicity/radiation effect or slower-growing tumors at baseline is difficult to determine [12,13,14]. Histologic examinations and in vitro studies of melanoma cell growth after pre-enucleation external beam radiation have found decreased mitotic and Ki-67 activity, and decreased cell adhesion, growth, and viability in cell culture, attributable to the radiation effect [15,16,17]. Meanwhile, another study found no mitotic figures and furthermore found evidence of “reproductive cell death” (defined as increased doubling time) in 3 fresh post-irradiation samples submitted for cell culture [18] (though it is worth noting that none of the samples submitted for cell culture were from eyes enucleated for suspicion of tumor regrowth).…”

Uveal melanoma: therapeutic consequences of doubling times and irradiation results; a review

“…Studies on pre-operatively irradiated human melanoma cells in tissue culture substantiate that irradiation indeed decreases the attachment, growth and viability of melanoma cells in these cultures, while microscopic examination indicated a reduced mitotic activity [7,16,37,63]. No beneficial effect of pre-enucleation irradiation could, however, be observed in terms of better survival after pre-enucleation irradiation as compared with control groups [9,15,40].…”

Effect of Pre-Enucleation Irradiation on the Survival of Patients with Uveal Melanoma

“…Studies in mice show that the probability of developing metastasis after pre-enucleation radiotherapy is low [7]. Although pre-enucleation radiotherapy seems to decrease the degree of mitotic activity in melanoma cells and to inhibit in vitro growth of tumor cells [8], available data suggest that it does not improve survival.…”