In vitro maturation of bovine oocytes in the presence of growth hormone accelerates nuclear maturation and promotes subsequent embryonic development

Izadyar, F.; Colenbrander, B.; Bevers, M.M.

doi:10.1002/(sici)1098-2795(199611)45:3<372::aid-mrd15>3.0.co;2-0

Cited by 112 publications

(71 citation statements)

References 29 publications

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“…The effects of GH on maturation of porcine oocytes were examined by Hagen and Graboski [16] who showed that low levels of GH accelerate cytoplasmic maturation as determined by activation by an electroporation stimulus. In cattle, pituitary GH present during in vitro maturation not only promotes nuclear maturation but also enhances blastocyst formation in embryos obtained after in vitro fertilization [17]. The stimulatory effect of GH on bovine oocyte maturation is dependent on the cumulus cells and is not mediated by insulin-like growth factor I (IGF-I) [18].…”

contrasting

confidence: 75%

“…It has been reported that the process of nuclear maturation of porcine [16] and rat [14] oocytes can be accelerated by adding GH to the culture medium. Culture of bovine oocytes in the presence of GH accelerated meiosis and increased the percentage of oocytes at the M-II stage after 22 h of culture, but the difference between GH-treated and untreat- ed oocytes had disappeared after 24 h of culture [17]. This ability of GH to accelerate oocyte maturation is dose-dependent and exerted through cumulus cells [14,18].…”

Section: Discussionmentioning

confidence: 99%

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Recombinant Bovine Growth Hormone Stimulates Nuclear Maturation of Bovine Oocytes In Vitro and Promotes Subsequent Embryonic Development.

1998

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Abstract. The present study was undertaken to examine the effects of recombinant bovine growth hormone (rbGH) on nuclear and cytoplasmic maturation of bovine oocytes in vitro and subsequent embryonic development after in vitro fertilization. When cumulus-oocyte complexes (COCs) and cumulus-free oocytes were cultured in modified Medium 199 supplemented with 0, 0.1, 1, 10, 100 or 1000 ng/ml rbGH for 24 h, no cumulus expansion was observed in most COCs in the presence of any of the tested concentrations of rbGH. The efficacy of nuclear maturation of oocytes to metaphase II was promoted by rbGH only in COCs showing higher proportions at 10-1000 ng/ml (77-78%) than at 0-1 ng/ml (57-64%). When COCs were cultured in the presence or absence of 10 ng/ml rbGH for 0-9 h, the proportions of oocytes that reached prometaphase I and metaphase I after 6 and 9 h of culture, respectively, were higher in the presence (53% and 51%, respectively) than in the absence (34% and 30%, respectively) of rbGH. After in vitro maturation and fertilization, no differences were observed in the penetration rate (82-91%), pronuclear formation (27-45%) and polyspermy (23-29%) at 8 h post-insemination among cumulus-enclosed oocytes cultured in the presence of different concentrations (0-1000 ng/ml) of rbGH. When starting 8 h after insemination, the oocytes were cultured for 136 h in a chemically defined medium, 21-25% of oocytes developed to the morula stage or beyond without difference among different concentrations of rbGH during oocyte maturation. However, at 192 h post-insemination higher proportions of oocytes developed to the blastocyst stage when COCs were matured in the presence (13-15%) than in the absence (6%) of 10-1000 ng/ml rbGH. These results indicate that a stimulatory effect of rbGH on bovine oocyte maturation is dependent on the cumulus cells. Presence of rbGH in the media improved maturation rate and subsequent embryonic development to the blastocyst stage.

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contrasting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Recombinant Bovine Growth Hormone Stimulates Nuclear Maturation of Bovine Oocytes In Vitro and Promotes Subsequent Embryonic Development.

1998

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“…At the end of maturation period, oocyte nuclear maturation was determined by DAPI-staining as described in details earlier [17]. Briefly, COCs were denuded by vortexing for 3-7 min and then fixed for 15 min in 2.5% (w/v) glutaraldehyde, washed with PBS, stained with 2.5%(w/v) 4,6-diamino-2-phenyl-indole (DAPI), and mounted on glass slides.…”

Section: Determination Of Oocyte Nuclear Maturation Statusmentioning

confidence: 99%

Unexpected detrimental effect of Insulin like growth factor-1 on bovine oocyte developmental competence under heat stress

Zhandi

Towhidi

Nasr-Esfahani

et al. 2009

J Assist Reprod Genet

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Purpose To investigate the effects of Insulin like growth factor-1 (IGF-1) on bovine oocyte developmental competence under heat stress. Methods In Experiment 1, bovine cumulus-oocyte complexes (COCs) were cultured at 38.5 or 41°C for the first 12 h of maturation in the presence of either 100 ng/ml human recombinant (hr)-IGF-1 or acetic acid. In Experiment 2, COCs were cultured in 38.5 or 41°C for the first 12 h of maturation in the presence either of 100 ng/ml hr-IGF-1 or acetic acid. After fertilization, putative zygotes were cultured for 8 days.Results In experiment 1, addition of rh-IGF-1 to maturation medium at 38.5°C significantly increased the proportion of M II oocytes and decreased the percentage of TUNEL-positive oocytes compared to the other groups. However, addition of rh-IGF-1 to maturation medium under heat stress increased the percentage of TUNEL-positive oocytes. In experiment 2, addition of rh-IGF-1 under heat sress did not affect cleavage rate, whereas, blastocyst formation rate decreased in heatstressed and heat-stressed plus rh-IGF-1 groups. Similarly, The number of trophectoderm cells and total cell number were decreased in heat-stressed and heat-stressed plus rh-IGF-1 groups and the percentage of TUNEL-positive nuclei were increased in heat-stressed and heat-stressed plus rh-IGF-1 groups compared to the other groups. Conclusion The results of the present study demonstrate that IGF-1 decreases oocyte developmental competence and total cell number and increases TUNEL-positive nuclei at heat stress condition. These unexpected results of IGF-1 during maturation period under heat stress condition warrant further optimizations and investigations.

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“…Acredita-se que o rbST pode alterar a morfologia da parede folicular ou do complexo cumulus-oócito, direta ou indiretamente, pela ação do IGF-1, resultando em impedimento da recuperação do oócito (Tripp et al, 2000). O rbST tem mostrado expandir as células do cumulus in vitro (Izadyar et al, 1996), o que pode ter contribuído para a retenção do oócito no folículo aspirado. A associação do rbST ao FSH (T3) pode ter evitado esse efeito da somatotropina por ter aumentado o número de folículos grandes e médios (Tabela 1), amenizando a retenção do oócito, ocasionada, possivelmente, por alterações nas células do cumulus.…”

Section: Resultsunclassified

“…O rbST promove aumento do IGF-1 no fluido folicular (Herrler et al, 1994), o que poderia melhorar a qualidade dos ovócitos e, conseqüentemente, a maturação e a formação in vitro de blastocistos. A adição de IGF-1 (Markkula & Makarevich, 2001) ou de rbST (Izadyar et al, 1996) ao meio da maturação in vitro tem auxiliado a maturação nuclear e a capacidade de desenvolvimento dos oócitos, refletidas no aumento da formação de blastocistos. Além disso, o uso da somatotropina pode preservar os ovócitos da degeneração atrésica (Pavlok et al, 1996).…”

Section: Introductionunclassified

Efeito da somatotropina na população folicular, recuperação de oócitos e produção in vitro de embriões em vacas Gir

et al. 2007

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RESUMO -Avaliou-se o efeito da pré-estimulação ovariana com somatotropina bovina recombinante (rbST), associada ou não ao FSH, sobre a população folicular, a recuperação de oócitos e a produção in vitro de embriões em vacas Gir. Utilizaram-se vacas não-lactantes, em boas condições reprodutiva e corporal, cujo ciclo estral foi sincronizado pela administração de 0,5 mg de cloprostenol. Durante o experimento, as vacas receberam continuamente implantes auriculares de norgestomet, renovados a cada 14 dias, e, posteriormente, foram submetidas aos tratamentos: T1 ou controle -punção sem tratamento pré-estimulatório; T2 -aplicação única de 160 mg de rbST antes de cada punção; e T3 -punção após o tratamento com 160 mg de rbST em aplicação única, seguida de administração de 250 UI de FSH em doses decrescentes. Os oócitos foram maturados e fertilizados in vitro e os presumíveis zigotos foram cultivados por 192 horas pós-fertilização. Os tratamentos não foram diferentes quanto à população folicular presente no ovário. Entretanto, com a administração de 250 UI de FSH (T3), verificou-se aumento no diâmetro do maior folículo e no número de folículos grandes e médios e diminuição no número de folículos pequenos. A taxa de recuperação não diferiu entre o tratamento controle e T3 (com administração de FSH), mas foi menor no T2 (aplicação, em dose única, de 150 mg de rbST antes de cada punção). O número de oócitos de grau 1 foi maior e o de degenerados, menor no T3 em relação ao T1 e ao T2. O rbST (T2) aumentou a taxa de clivagem e a produção de blastocistos e, quando associado ao FSH, melhorou a qualidade dos oócitos nos animais Gir.Palavras-chave: bovinos, Gir, punção folicular, rbST Effect of somatotropin on follicular population, oocyte retrieval and in vitro embryo production in Gir cowsABSTRACT -The aim of this study was to evaluate ovarian pre-stimulation using recombinant bovine somatotropin (rbST) or rbST associated to FSH on follicular population, oocyte retrieval and in vitro embryo production in Gir cows (Bos indicus). Estrous cycle of non-lactating cycling cows with similar body and reproductive conditions were synchronized with cloprostenol followed by norgestomet ear implants, replaced every fourteen days. The animals were randomly distributed in three treatments: TI or control (punctured without pre-stimulation), TII (injection of 160 mg of rbST, before each aspiration) and TIII (injection of 160 mg of rbST followed by administration of 250 U.I. of FSH, before each aspiration). Oocytes were maturated and fertilized in vitro and presumptive zygotes were cultured in vitro for 192 hours post-fertilization. Treatments did not differ for ovarian follicular population. However, an increase in the diameter of the largest follicle and in the number of large and medium follicles with reduction in the number of small follicles was observed for TIII. Oocyte recovery did not differ between TI and TIII, but recovery rate was lower for TII. The number of grade I oocytes was higher and the number of degenerated oocytes was lower ...

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In vitro maturation of bovine oocytes in the presence of growth hormone accelerates nuclear maturation and promotes subsequent embryonic development

Cited by 112 publications

References 29 publications

Recombinant Bovine Growth Hormone Stimulates Nuclear Maturation of Bovine Oocytes In Vitro and Promotes Subsequent Embryonic Development.

Recombinant Bovine Growth Hormone Stimulates Nuclear Maturation of Bovine Oocytes In Vitro and Promotes Subsequent Embryonic Development.

Unexpected detrimental effect of Insulin like growth factor-1 on bovine oocyte developmental competence under heat stress

Efeito da somatotropina na população folicular, recuperação de oócitos e produção in vitro de embriões em vacas Gir

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