In vitro laser radiation induces mutation and growth in Eustoma grandiflorum plant

Abstract

Background: Eustoma grandiflorum is a new floral crop for the international flowers market, moderately coldtolerant annual or biennial plant. A large number of seedlings can be produced by seed propagation but the quality is not uniform due to variations in the flowering time, plant height, and the number of flowers. Propagation of Eustoma grandiflorum plant by tissue culture technique is relatively low. Inducing mutations is one of the powerful tools for breeding biotechnology. Laser mutagenesis is an easy and new tool. The goal of the present work was to investigate the influence of laser irradiation on in vitro growth, anatomy, flowering, chemicals composition, and gene mutagenesis. Results: The most of morphological, floral parameters, total chlorophyll, carotenoids, and anthocyanin pigment contents in the flower recorded increment by most treatments of laser types. The highest survival percentage of acclimatized plants (95%) and highest values of number of branches and branches length (cm) were obtained from treated plantlets by 20 min of green laser, while most of highest floral parameters, anthocyanin pigment contents in flower, and anatomical structural parameters recorded increasing using 20 min of blue laser and 20, 25 min of green and red laser, respectively. Contrary, the lowest values of photosynthetic pigments and carotenoids were obtained from 20 min of green laser. Conclusions: The current research concluded that laser irradiation has remarkable effect on plant morphology, flowering, chemical constituents, and gene mutagenesis.

Bio-Stimulant for Improving Simmondsia chinensis Secondary Metabolite Production, as Well as Antimicrobial Activity and Wound Healing Abilities

“…The multiplication stage plant growth increase in line 8 is accompanied by a significant increase in rooting characteristics and plant photosynthetic pigments. Laser bio-stimulation effects on plant growth were reported on S. chinensis [ 9 ] and eventually quoted by researchers in different plant genera [ 26 , 27 ]. Within the three irradiation periods tested in this study, the 10 min pre-sowing irradiation lines produced the highest growth.…”

Differing In Vitro Rooting and Flowering Responses of the Persian Violet to Low and High UV-C Irradiation

“…The use of different radiation sources has recently made available new choices for rooting in in vitro explant culturing. Many kinds of irradiation, for example, 350-740 nm radiation [20], gammaray [21], and laser [22], have assisted in adventitious root induction and development in various plant species. This method for in vitro rooting without chemical utilization can effectively reduce the cost of the culture medium.…”

A Laser Irradiation Method for Controlling Pieris rapae Larvae

“…Since their invention, lasers have been used for many applications; in fact, lasers are a high-tech tool used to meet the challenges of sustainable agriculture [5]. For instance, lasers can be used as biostimulator devices, and studies have shown that laser irradiation can stimulate various plant organs and tissues [6][7][8]. The irradiation of seeds, seedlings or plants by a low-intensity laser can improve their yield and quality due to stimulation of their biochemical and physiological processes [9,10].…”