In vitro infection by Leishmania infantum in the peripheral blood mononuclear cell-derived macrophages from crab-eating foxes ( Cerdocyon thous )

Tuñon, Gabriel Isaias Lee; Moura, Tatiana Rodrigues de; Jesus, Amélia Ribeiro de; Almeida, Roque Pacheco de

doi:10.1016/j.vetpar.2015.06.027

Cited by 3 publications

(1 citation statement)

References 26 publications

(27 reference statements)

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“…L. tarentolae has already been shown to be suitable to be phagocyted by human dendritic cells (13); our results indicate that this parasite can also be engulfed by THP-1-derived macrophage cells, in coherence with previous results with L. infantum on the same cell line (22), or in monocyte-derived, canine macrophages (43); in addition, L. tarentolae has also been shown to be phagocyted by canine macrophages (44). Notably, in WT THP-1-derived macrophages, the percentage of ASC-speckpositive cells was significantly reduced upon L. tarentolae infection.…”

Section: Discussionsupporting

confidence: 91%

The non-pathogenic protozoon Leishmania tarentolae interferes with the activation of NLRP3 inflammasome in human cells: new perspectives in the control of inflammation

La Rosa,

Varotto-Boccazzi,

Saresella

et al. 2024

Front. Immunol.

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BackgroundInnate immune responses against infectious agents can act as triggers of inflammatory diseases. On the other hand, various pathogens have developed mechanisms for the evasion of the immune response, based on an inhibition of innate immunity and inflammatory responses. Inflammatory diseases could thus be controlled through the administration of pathogens or pathogen-derived molecules, capable of interfering with the mechanisms at the basis of inflammation. In this framework, the NLRP3 inflammasome is an important component in innate antimicrobial responses and a major player in the inflammatory disease. Parasites of the genus Leishmania are master manipulators of innate immune mechanisms, and different species have been shown to inhibit inflammasome formation. However, the exploitation of pathogenic Leishmania species as blockers of NLRP3-based inflammatory diseases poses safety concerns.MethodsTo circumvent safety issues associated with pathogenic parasites, we focused on Leishmania tarentolae, a species of Leishmania that is not infectious to humans. Because NLRP3 typically develops in macrophages, in response to the detection and engulfment microorganisms, we performed our experiments on a monocyte-macrophage cell line (THP-1), either wild type or knockout for ASC, a key component of NLRP3 formation, with determination of cytokines and other markers of inflammation.ResultsL. tarentolae was shown to possess the capability of dampening the formation of NLRP3 inflammasome and the consequent expression of pro-inflammatory molecules, with minor differences compared to effects of pathogenic Leishmania species.ConclusionThe non-pathogenic L. tarentolae appears a promising pro-biotic microbe with anti-inflammatory properties or a source of immune modulating cellular fractions or molecules, capable of interfering with the formation of the NLRP3 inflammasome.

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Section: Discussionsupporting

confidence: 91%

The non-pathogenic protozoon Leishmania tarentolae interferes with the activation of NLRP3 inflammasome in human cells: new perspectives in the control of inflammation

La Rosa,

Varotto-Boccazzi,

Saresella

et al. 2024

Front. Immunol.

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Intracellular persistence of Leishmania tarentolae in primary canine macrophage cells

et al. 2023

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Isolation of Clostridium perfringens and C. difficile in crab-eating fox ( Cerdocyon thous - Linnaeus 1776) from Northeastern Brazil

Almeida

Silva

Lobato

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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The aim of the present study was to isolate Clostridium perfringens and C. difficile in crab-eating fox (Cerdocyon thous) from Northeastern Brazil. Stool samples of 18 captive crab-eating foxes from four states of Northeastern Brazil (Alagoas, Bahia, Paraíba e Pernambuco) were collected and subjected to C. perfringens and C. difficile isolation. Suggestive colonies of C. perfringens were then analyzed for genes encoding the major C. perfringens toxins (alpha, beta, epsilon and iota), beta-2 toxin (cpb2), enterotoxin (cpe), and NetB- (netB) and NetF- (netF) encoding genes. C. difficile strains were analyzed by multiplex-PCR for a housekeeping gene (tpi), toxins A (tcdA) and B (tcdB) and a binary toxin gene (cdtB). Unthawed aliquots of stool samples positive for toxigenic C. difficile were subjected to a commercial ELISA to evaluate the presence of A/B toxins. Clostridium perfringens (type A) was isolated from five (27%) samples, and only one sample was positive for beta-2 enconding gene (cpb2). Two (11%) stool samples were positive for C. difficile, but negative for A/B toxins. These two wild canids were also positive for C. perfringens type A. This is the first report of C. difficile in crab-eating fox.

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In vitro infection by Leishmania infantum in the peripheral blood mononuclear cell-derived macrophages from crab-eating foxes ( Cerdocyon thous )

Cited by 3 publications

References 26 publications

The non-pathogenic protozoon Leishmania tarentolae interferes with the activation of NLRP3 inflammasome in human cells: new perspectives in the control of inflammation

The non-pathogenic protozoon Leishmania tarentolae interferes with the activation of NLRP3 inflammasome in human cells: new perspectives in the control of inflammation

Intracellular persistence of Leishmania tarentolae in primary canine macrophage cells

Isolation of Clostridium perfringens and C. difficile in crab-eating fox ( Cerdocyon thous - Linnaeus 1776) from Northeastern Brazil

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