In vitro induction of tetraploids in ornamental Ranunculus

Dhooghe, Emmy; Denis, Sylvie; Eeckhaut, Tom; Reheul, Dirk; Labeke, Marie-Christine Van

doi:10.1007/s10681-008-9876-1

Cited by 44 publications

(32 citation statements)

References 37 publications

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“…Mixoploid induction has been reported in Ranunculus asiaticus [14], Echinacea purpurea [18], and Pyrus pyrifolia N. cv. Hosui [26].…”

Section: Discussionmentioning

confidence: 96%

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In Vitro Tetraploid Induction from Leaf and Petiole Explants of Hybrid Sweetgum (Liquidambar styraciflua × Liquidambar formosana)

Yan

ZeWei

et al. 2017

Forests

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Abstract:Liquidambar is an important forestry species used to generate many commercial wood products, such as plywood. Inducing artificial polyploidy is an effective method to encourage genetic enhancements in forestry breeding. This report presents the first in vitro protocol for the induction of genus Liquidambar tetraploids based on the established in vitro regeneration system of hybrid sweetgum (Liquidambar styraciflua × Liquidambar formosana). The leaves and petioles from three genotypes were pre-cultured in woody plant medium (WPM) supplemented with 0.1 mg/L thidiazuron (TDZ), 0.8 mg/L benzyladenine (BA), and 0.1 mg/L α-naphthalene acetic acid (NAA) for a variable number of days (4, 6 or 8 days), and exposed to varying concentrations of colchicine (120, 160, 200 mg/L) for 3, 4 or 5 days; the four factors were investigated using an orthogonal experimental design. Adventitious shoots were rooted in 1/2 WPM medium supplemented with 2.0 mg/L indole butyric acid (IBA) and 0.1 mg/L NAA. The ploidy level was assessed using flow cytometry and chromosome counting. Four tetraploids and nine mixoploids were obtained from the leaves. Pre-treatment of the leaves for 8 days and exposure to 200 mg/L colchicine for 3 days led to the most efficient tetraploid induction. Producing 11 tetraploids and five mixoploids from petioles, the best tetraploid induction treatment for petioles was almost the same as that with the leaves, except that pre-culturing was required for only 6 days. In total, 15 tetraploids were obtained with these treatments. This study described a technique for the induction of tetraploid sweetgum from the leaves or petioles of parental material. Based on the success of polyploid breeding in other tree species, the production of hybrid sweetgum allotetraploids constitutes a promising strategy for the promotion of future forestry breeding.

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“…Mixoploid induction has been reported in Ranunculus asiaticus [14], Echinacea purpurea [18], and Pyrus pyrifolia N. cv. Hosui [26].…”

Section: Discussionmentioning

confidence: 96%

“…Successful induction of tetraploids has been achieved using various chemical reagents, such as colchicine [12], oryzalin [13], and trifluralin [14]. Colchicine is the most widely applied chemical for in vitro tetraploid induction.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Tetraploid Induction from Leaf and Petiole Explants of Hybrid Sweetgum (Liquidambar styraciflua × Liquidambar formosana)

Yan

ZeWei

et al. 2017

Forests

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“…acuminata 'Kluai Leb Mu Nang' and 'Kluai Sa' are characterized by diploid genomes hence polyploidy can be achieved by treatment with antimitotic agents such as colchicine, oryzalin and trifluralin. According to Dhooghe et al (2009) oryzalin might be used at lower concentrations compared to colchicine and trifluralin, and therefore, cytotoxic side effects can be minimized. Oryzalin has been used for induction of tetraploids in many crops i.e., Alocasia (Phuong Thao et al 2003), Citrus (Aleza et al 2009), Rosa (Kermani et al 2003), Solanum (Chauvin et al 2003).…”

Section: Introductionmentioning

confidence: 99%

In vitro induction of tetraploid plants from callus cultures of diploid bananas (Musa acuminata, AA group) ‘Kluai Leb Mu Nang’ and ‘Kluai Sa’

Kanchanapoom

Koarapatchaikul

2011

Euphytica

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Tetraploid plants were induced successfully from diploid bananas Musa acuminata (AA genome) 'Kluai Leb Mu Nang' and 'Kluai Sa' (2n = 2x = 22) with in vitro oryzalin treatment. Calluses from in vitro-grown shoot tips of both cultivars were treated with oryzalin at concentrations of 1.5 or 3 mg l -1 for 24, 48 and 72 h, respectively. The oryzalin treatments produced tetraploids at a frequency of 15.6% in 'Kluai Leb Mu Nang' and 16.7% in 'Kluai Sa' as detected by flow cytometry. Chromosome counting showed that the tetraploid plant chromosome number was (2n = 4x = 44). The selected tetraploid plants were transplanted in the field and variations in the morphological characteristic of leaf shape and fruit bunch compared to normal diploid plants were found under the same growing condition even after 3 years of cultivation.

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“…For instance, higher ploidy level was associated with increased flower size in rhododendron and intensified flower colors in carnation and cyclamen [5,6] and this manipulation in ploidy is also significant for genetic improvement of many plants [7] often generates variants that may possess useful characteristics and by doubling the gene products it also provides a wider germplasm base for breeding studies. Many researchers have induced polyploidy in several plant species through the antimitotic chemical, colchicine (C 22 H 25 NO 6 ) [8][9][10] which is obtained from the seeds and bulbs of the plant Colchicum autumnale L. More importantly without damaging any other life processes of the chromosomes, colchicine effects a temporary inactivation of the spindle mechanism, both the exterior part working from the centrosomes and the interior centromeric part [11]. Among various antimicrotubular drugs colchicine has proven to be the most useful to double the chromosome numbers of numerous crop species, including decorative flowers, medical and agricultural plants [12].…”

mentioning

confidence: 99%