In vitro grown human ovarian follicles from cancer patients support oocyte growth

Xu, Min; Barrett, Susan L.; West-Farrell, Erin R.; Kondapalli, Laxmi A.; Kiesewetter, Sarah E.; Shea, Lonnie D.; Woodruff, Teresa K.

doi:10.1093/humrep/dep228

Cited by 285 publications

(244 citation statements)

References 65 publications

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“…Until now, however, the production of live offspring from primordial and pre-antral follicles from cryopreserved/thawed ovarian tissue and cultured to mature in vitro has only been reported in immature mice (Liu et al 2001, O'Brien et al 2003, Hasegawa et al 2006. No live births have previously been reported using adult mouse ovaries, and although development of follicles to the antral stage has been observed in human ovarian tissue cultured in vitro (Isachenko et al 2003, Xu et al 2009, there have been no reports assessing the competency of the oocytes obtained from these in vitro grown follicles.…”

Section: Discussionmentioning

confidence: 99%

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Wang¹,

Catt²,

Pangestu³

et al. 2011

Reproduction

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Cryopreservation of ovarian tissue is an important option for preserving the fertility of cancer patients undergoing chemotherapy and radiotherapy. In this study, we examined the viability and function of oocytes derived in vitro from pre-antral follicles as an alternative method for restoring fertility. Pre-antral follicles (specified as secondary follicle with a diameter around 100-130 mm) were mechanically isolated from vitrified-warmed and fresh adult mouse ovarian tissues and cultured for 12 days followed by an ovulation induction protocol at the end of this period to initiate oocyte maturation. Oocytes were then released from these follicles, fertilized in vitro, and cultured to the blastocyst stage and vitrified. After storage in liquid nitrogen for 2 weeks, groups of vitrified blastocysts were warmed and transferred into pseudo-pregnant recipient females. Although most of the isolated mouse pre-antral follicles from fresh (79.4%) and vitrified (75.0%) ovarian tissues survived the 12-day in vitro culture period, significantly fewer mature oocytes developed from vitrifiedwarmed pre-antral follicles than from the fresh controls (62.2 vs 86.4%, P!0.05). No difference was observed in embryo cleavage rates between these two groups, but the proportion of embryos that developed into blastocysts in the vitrification group was only half that of the controls (24.2 vs 47.2%, P!0.05). Nevertheless, live births of healthy normal pups were achieved after transfer of vitrified blastocysts derived from both experimental groups. This study shows that successful production of healthy offspring using an in vitro follicle culture system is feasible, and suggests that this procedure could be used in cancer patients who wish to preserve their fertility using ovarian tissue cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Wang¹,

Catt²,

Pangestu³

et al. 2011

Reproduction

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“…Isolated primordial follicles were encapsulated in either 0.5 % or 2 % alginate (Sigma-Aldrich) in phosphatebuffered saline (PBS) as previously described [36]. Briefly, groups of follicles were transferred to alginate, and beads were formed by pipetting drops of alginate into the cross-linking solution (50 mM CaCl 2 and 140 mM NaCl).…”

Section: Human Ovarian Tissue Acquisition and Transportmentioning

confidence: 99%

“…To overcome the limitations of ovarian tissue transplantation, an alternative fertility preservation approach, in vitro follicle growth (IVFG), is actively being developed [35,36]. In this method, ovarian tissue or follicles isolated from ovarian tissue can be grown in vitro to produce mature gametes that could ultimately be used in standard ART procedures.…”

Section: Introductionmentioning

confidence: 99%

Alginate encapsulation supports the growth and differentiation of human primordial follicles within ovarian cortical tissue

Laronda

Duncan

Hornick

et al. 2014

J Assist Reprod Genet

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112

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“…Our basal 3D culture system employs the biomaterial alginate, which has been used successfully in the culture of murine, monkey, and human immature follicles [7][8][9][10][11]. Compared with 2D culture systems, follicles cultured in the alginate hydrogel have similar growth trends in follicle diameter and steroid production, yet produce a significantly greater percentage of mature oocytes.…”

mentioning

confidence: 99%

A new hypothesis regarding ovarian follicle development: ovarian rigidity as a regulator of selection and health

Woodruff

Shea

2010

J Assist Reprod Genet

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142

125

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The mammalian ovary consists of a large number of dormant immature follicles, each containing a single oocyte and located on the periphery of the ovary. With each reproductive cycle, a group of immature follicles is sequentially activated to resume growth, and pituitary gonadotropins and ovarian steroid and peptide hormones cooperate to ensure further growth and development. A single dominant follicle eventually emerges, ovulates, and then involutes to allow the selection of the next group of follicles. While hormones are known to control the later stages of folliculogenesis, little is known about the pathways that activate individual immature primordial follicles in the dormant follicle pool. We advance a new hypothesis: that follicle activation is dependent on the physical environment of the ovary in addition to well-established hormonal cues. This novel perspective on ovarian function may provide new avenues to study follicle dynamics and identify therapeutic targets for ovarian dysfunction.Keywords Ovary . Follicle . Endocrine . Alginate . BiomaterialsThe role of hormones, architecture and a predicted role for the physical environment in the control of follicle development Hormone-and growth factor-driven follicle growth The initial activation and development of primordial follicles to the primary and early secondary stages is generally considered to occur independent of pituitary gonadotropin [folliclestimulating hormone (FSH) and luteinizing hormone (LH)] control [1,2]. These follicles lack gonadotropin receptors and are distal to ovarian blood supply, and are thus not influenced by the fluctuation of hormone levels that occurs with each Capsule Follicle development is controlled by hormones, the 3D relationship between oocyte and somatic cells and the physical environment of the ovary.

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In vitro grown human ovarian follicles from cancer patients support oocyte growth

Abstract: Our data support the notion that human follicle development can be achieved in vitro in a bio-engineered culture system. More studies are required to investigate whether the fully sized oocytes obtained from in vitro grown follicle are competent to resume meiosis and be fertilized.

Cited by 285 publications

References 65 publications

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Alginate encapsulation supports the growth and differentiation of human primordial follicles within ovarian cortical tissue

A new hypothesis regarding ovarian follicle development: ovarian rigidity as a regulator of selection and health

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