In vitro expression of full-length and truncated bovine respiratory syncytial virus G proteins and their antibody responses in BALB/c mice

Brady, Ryan P; Topliff, Christina L.; Kelling, Clayton L.

doi:10.1016/j.vaccine.2004.03.020

Cited by 3 publications

(4 citation statements)

References 37 publications

(24 reference statements)

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“…Few investigators have cloned and expressed full length/partial G protein gene of hRSV [20][21][22][23][24][25][26] . These studies focused either the antigenic characterization of G protein or cloning of G protein for development of diagnostic reagents.…”

Section: Introductionmentioning

confidence: 99%

Biophysical characterization of G protein ectodomain of group B human respiratory syncytial virus fromE. coli

Khan

Srungaram

Islam

et al. 2015

Preparative Biochemistry and Biotechnology

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Human respiratory syncytial virus (hRSV) is an important pathogen of acute respiratory tract infection. The G protein of hRSV is a transmembrane glycoprotein that is a neutralizing antigen and is thus a vaccine candidate. In this study, synthetic codon optimized ectodomain G protein [G(ΔTM)] of BA genotype of group B hRSV was cloned, expressed, and characterized using biophysical techniques. The molar absorption coefficient and mean residue ellipticity at 222 nm ([θ]222) of G (ΔTM) was found to be 7950 M(-1) cm(-1) and -19701.7 deg cm(2) dmol(-1) respectively. It was concluded that G(ΔTM) mainly consist of α-helix (74.9%) with some amount of β-sheet (4%). The protein was stable up to 85°C without any transition curve. However, heat-induced denaturation of G(ΔTM) resulted in total loss of β-sheet whereas not much change was observed in the α-helix part of the secondary structure. It was concluded that G(ΔTM) is an α-helical protein and it is highly stable at high temperature, but could be easily denatured using high concentrations of GdmCl/urea or acidic condition. This is the first investigation of cloning, expression, and characterization of G(ΔTM) of BA viruses from India. Structural characterization of G protein will assist in drug designing and vaccine development for hRSV.

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Section: Introductionmentioning

confidence: 99%

Biophysical characterization of G protein ectodomain of group B human respiratory syncytial virus fromE. coli

Khan

Srungaram

Islam

et al. 2015

Preparative Biochemistry and Biotechnology

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“…Furthermore, DNA immunization might avoid the inhibitory effect of maternal antibodies (for reviews, see references 21, 22, and 44). Efficacy of DNA immunization against RSV F and G proteins has well been described in rodents (6,8,32,33,58). However, immunization of large animals like cattle or monkeys offered only partial protection and require more innovative approaches, like codon optimization and protein boost following DNA vaccination (48,53,64).…”

Section: Discussionmentioning

confidence: 99%

“…So far, DNA vaccination against HRSV has been mainly investigated in mice or cotton rats (6,8,32,33,58). These studies demonstrated that plasmids encoding the HRSV fusion (F) or attachment (G) proteins primed both humoral and cell-mediated immunity and protected against HRSV infection without significantly enhancing pulmonary pathology following challenge.…”

mentioning

confidence: 99%

DNA Immunization with Plasmids Encoding Fusion and Nucleocapsid Proteins of Bovine Respiratory Syncytial Virus Induces a Strong Cell-Mediated Immunity and Protects Calves against Challenge

Boxus

Tignon

Roels

et al. 2007

J Virol

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Respiratory syncytial viruses (RSV) are one of the most important respiratory pathogens of humans and cattle, and there is currently no safe and effective vaccine prophylaxis. In this study, we designed two codon-optimized plasmids encoding the bovine RSV fusion (F) and nucleocapsid (N) proteins and assessed their immunogenicity in young calves. Two administrations of both plasmids elicited low antibody levels but primed a strong cell-mediated immunity characterized by lymphoproliferative response and gamma interferon production in vitro and in vivo. Interestingly, this strong cellular response drastically reduced viral replication, clinical signs, and pulmonary lesions after a highly virulent challenge. Moreover, calves that were further vaccinated with a killed-virus vaccine developed high levels of neutralizing antibody and were fully protected following challenge. These results indicate that DNA vaccination could be a promising alternative to the classical vaccines against RSV in cattle and could therefore open perspectives for vaccinating young infants.

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“…DNA vaccination is an effective way of generating humoral and cell-mediated response [34]. Intramuscular vaccination of mice with plasmid DNA expressing the F or G proteins of RSV has shown to induce a strong TH1 response and to reduce the viral load in the lungs after RSV challenge, without inducing enhanced pulmonary inflammatory response or eosinophilia [35,36]. However, it is generally recognized that DNA vaccines are often less effective in large animals than in mice [37].…”

Section: Introductionmentioning

confidence: 99%

Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime–protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology

Letellier

Boxus

Rosar

et al. 2008

Vaccine

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Respiratory syncytial virus (RSV) is a major cause of respiratory disease in both cattle and young children. Despite the development of vaccines against bovine (B)RSV, incomplete protection and exacerbation of subsequent RSV disease have occurred. In order to circumvent these problems, calves were vaccinated with the nucleocapsid protein, known to be a major target of CD8(+) T cells in cattle. This was performed according to a DNA prime-protein boost strategy. The results showed that DNA vaccination primed a specific T-cell-mediated response, as indicated by both a lymphoproliferative response and IFN-gamma production. These responses were enhanced after protein boost. After challenge, mock-vaccinated calves displayed gross pneumonic lesions and viral replication in the lungs. In contrast, calves vaccinated by successive administrations of plasmid DNA and protein exhibited protection against the development of pneumonic lesions and the viral replication in the BAL fluids and the lungs. The protection correlated to the cell-mediated immunity and not to the antibody response.

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In vitro expression of full-length and truncated bovine respiratory syncytial virus G proteins and their antibody responses in BALB/c mice

Cited by 3 publications

References 37 publications

Biophysical characterization of G protein ectodomain of group B human respiratory syncytial virus fromE. coli

Biophysical characterization of G protein ectodomain of group B human respiratory syncytial virus fromE. coli

DNA Immunization with Plasmids Encoding Fusion and Nucleocapsid Proteins of Bovine Respiratory Syncytial Virus Induces a Strong Cell-Mediated Immunity and Protects Calves against Challenge

Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime–protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology

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