In vitro exposure of tracheobronchial epithelial cells and of tracheal explants to ozone

Impact of Surfactant Protein-A Variants on Survival in Aged Mice in Response to Klebsiella pneumoniae Infection and Ozone: Serendipity in Action

“…In our system [ 23 , 82 ], with an exposure vessel size of 3.66 L, the flow rate we used resulted in four complete air changes/minute, which is considerably higher than that used earlier in other similar systems. Also of note is that the FA is humidified and warmed to a temperature of 37.5 °C, prior to delivery into the exposure vessel, and the exposure vessel is maintained at a constant positive pressure of 1.5 cm H 2 O relative to the surroundings, to protect against contamination in the event of a leak and as a method for detection of leaks [ 82 , 113 , 114 ].…”

Impact of Surfactant Protein-A Variants on Survival in Aged Mice in Response to Klebsiella pneumoniae Infection and Ozone: Serendipity in Action

“…In our system [ 23 , 82 ], with an exposure vessel size of 3.66 L, the flow rate we used resulted in four complete air changes/minute, which is considerably higher than that used earlier in other similar systems. Also of note is that the FA is humidified and warmed to a temperature of 37.5 °C, prior to delivery into the exposure vessel, and the exposure vessel is maintained at a constant positive pressure of 1.5 cm H 2 O relative to the surroundings, to protect against contamination in the event of a leak and as a method for detection of leaks [ 82 , 113 , 114 ].…”

Interactions of nanoparticles with pulmonary structures and cellular responses

“…However, the particle dose added does not necessarily reflect the particle dose in contact with the cells, since the main mode of NSP motion is Brownian diffusion, which means that the particles do not sediment on the cells (70). In addition, the decrease in cell viability in a bronchial epithelial cell line upon exposure to NO 2 was inversely related to the amount of fluid present on the cells (137). Therefore, exposure of the cell cultures at the air-liquid interface is preferable to submerse exposure.…”

Oxidized Phospholipids Derived from Ozone-Treated Lung Surfactant Extract Reduce Macrophage and Epithelial Cell Viability

“…One of these systems is always used for an air control while the other three can be used for exposure of materials or cells to a variety of ozone concentrations. The material to be exposed to each concentration of ozone is placed within a specially designed (31) glass exposure chamber of 3.66 L volume. Mass flow controllers (Sierra Instruments, Monterey, CA) are used to mix filtered dry air with compressed carbon dioxide to achieve a 5% CO2 concentration.…”

Impact of Surfactant Protein-A Variants on Survival in Aged Mice in Response to Klebsiella pneumoniae Infection and Ozone: Serendipity in Action

“…In our system [ 23 , 82 ], with an exposure vessel size of 3.66 L, the flow rate we used resulted in four complete air changes/minute, which is considerably higher than that used earlier in other similar systems. Also of note is that the FA is humidified and warmed to a temperature of 37.5 °C, prior to delivery into the exposure vessel, and the exposure vessel is maintained at a constant positive pressure of 1.5 cm H 2 O relative to the surroundings, to protect against contamination in the event of a leak and as a method for detection of leaks [ 82 , 113 , 114 ].…”

Interactions of nanoparticles with pulmonary structures and cellular responses

“…However, the particle dose added does not necessarily reflect the particle dose in contact with the cells, since the main mode of NSP motion is Brownian diffusion, which means that the particles do not sediment on the cells (70). In addition, the decrease in cell viability in a bronchial epithelial cell line upon exposure to NO 2 was inversely related to the amount of fluid present on the cells (137). Therefore, exposure of the cell cultures at the air-liquid interface is preferable to submerse exposure.…”

Oxidized Phospholipids Derived from Ozone-Treated Lung Surfactant Extract Reduce Macrophage and Epithelial Cell Viability

“…One of these systems is always used for an air control while the other three can be used for exposure of materials or cells to a variety of ozone concentrations. The material to be exposed to each concentration of ozone is placed within a specially designed (31) glass exposure chamber of 3.66 L volume. Mass flow controllers (Sierra Instruments, Monterey, CA) are used to mix filtered dry air with compressed carbon dioxide to achieve a 5% CO2 concentration.…”

Impact of Surfactant Protein-A Variants on Survival in Aged Mice in Response to Klebsiella pneumoniae Infection and Ozone: Serendipity in Action

“…In our system [ 23 , 82 ], with an exposure vessel size of 3.66 L, the flow rate we used resulted in four complete air changes/minute, which is considerably higher than that used earlier in other similar systems. Also of note is that the FA is humidified and warmed to a temperature of 37.5 °C, prior to delivery into the exposure vessel, and the exposure vessel is maintained at a constant positive pressure of 1.5 cm H 2 O relative to the surroundings, to protect against contamination in the event of a leak and as a method for detection of leaks [ 82 , 113 , 114 ].…”

Interactions of nanoparticles with pulmonary structures and cellular responses

“…However, the particle dose added does not necessarily reflect the particle dose in contact with the cells, since the main mode of NSP motion is Brownian diffusion, which means that the particles do not sediment on the cells (70). In addition, the decrease in cell viability in a bronchial epithelial cell line upon exposure to NO 2 was inversely related to the amount of fluid present on the cells (137). Therefore, exposure of the cell cultures at the air-liquid interface is preferable to submerse exposure.…”

Oxidized Phospholipids Derived from Ozone-Treated Lung Surfactant Extract Reduce Macrophage and Epithelial Cell Viability

“…One of these systems is always used for an air control while the other three can be used for exposure of materials or cells to a variety of ozone concentrations. The material to be exposed to each concentration of ozone is placed within a specially designed (31) glass exposure chamber of 3.66 L volume. Mass flow controllers (Sierra Instruments, Monterey, CA) are used to mix filtered dry air with compressed carbon dioxide to achieve a 5% CO2 concentration.…”